Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells

Abstract

A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fractions of Amorpha fruticosa LINNE. Both of the fractions and purified single compound showed a 19% relatively low cytotoxicity at a maximum concentration of 1.0 g/L in a cultivated normal human lung cell line (HEL299). The purified single compound showed less cytotoxicity than the crude extracts, possibly because residual toxicants were eliminated during purification processes. Cell growth of human T cells was increased by about 15% by adding 0.5 g/L of the fractions compared to the control. Specific production rates of interleukin-6 (IL-6) and tumor necrosis factor (TNF-alpha) from T cell were higher as 1.16 x 10(-4) and 1.86 x 10(-4 )pg/cell, respectively, in the purified compound, compared to 1.38 x 10(-4) and 2.22 x 10(-4 )pg/cell, respectively, by adding 0.5 g/L of the dichloromethane fraction. Natural killer cell-92MI (NK-92MI) growth supplemented with the supernatant of human T cell was up to 19% higher with the dichloromethane fraction compared with a new single compound at a concentration of 0.5 g/L. Overall, the dichloromethane fraction showed relatively higher immuno-modulatory activities compared with a new single compound, probably due to the synergic effect given by other substances existing in the fractions.

The Potential Use of Indigobush (Amorpha fruticosa L.) as Natural Resource of Biologically Active Compounds

“…There was no stimulatory effect on HeLa cells, except at the highest concentration of Indigobush extract (10 mg•mL -1 ). Similarly, the positive effect on the growth of human T-cells (approximately +15%) was observed with the addition of 0.5 g•L -1 of selected Indigobush fruit extracts compared to the control [8]. In the same study, the methanol fruit extract of Indigobush showed relatively low cytotoxicity of 19% at a maximum concentration of 1 g•L -1 in normal human lung cell line (HEL299).…”

“…The Indigobush beans gland contains about 3.5% of oil which is composed of various terpenes. The Indigobush seed is composed of fatty acids including linoleic, oleic, palmitic and stearic acid [6][7][8]. In 1943 glycoside amorphin, which is applied as a drug for nerve and heart disease, was isolated from Indigobush fruits [1].…”

Bovine male germline stem-like cells cultured in serum- and feeder-free medium

“…The seminiferous tubules from each testis were separated into small pieces using forceps. Seminiferous epithelial cells were dissociated by modified enzymatic digestion and plated by two-step successive differential plating methods (Izadyar et al 2003a, b;Lee et al 2006). The cells were collected and cultured for 12 h, respectively, at 37°C by two-step successive differential plating methods.…”

Bonellia albiflora: A Mayan Medicinal Plant That Induces Apoptosis in Cancer Cells

“…In recent years, not only has the study of medically bioactive compounds from plants become more frequent, but also that of the plant extracts themselves or the mixture of compounds that together could yield better biological activity than that exhibited by a single compound has, moreover, become frequent [19]. In this work, the components are described as the fingerprinting of HFBa performed by GC-MS for future standardizations.…”