Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland

Abstract

A new single nucleotide polymorphism was revealed using PCR-SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A-->G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR-RFLP genotyping. The application of real-time PCR revealed that the prolactin gene expression level in the pituitary was higher in cattle with the AA genotype than in those with the GG genotype. EMSA analysis, however, showed increased nuclear protein binding to the sequence variant with G, suggesting a possible inhibition event, in which the transcription factors Pit1, Oct1, and YY1 could be involved.

The association of five polymorphisms with milk production traits in Czech Fleckvieh cattle

“…The genotypic and allelic frequencies of the PRLE polymorphism were similar to results reported for the Holstein-Friesian breed in Poland (Brym et al, 2007) and China (Lü et al, 2010). In the pituitary gland, the prolactin gene expression level is higher in animals with the AA genotype than in those with the GG genotype; this is presumably due to variation in the affinity of the enhancer site to transcription factors (Brym et al, 2007). In our study, the genotype GG was associated with higher MY, which also influenced the FY and PY and breeding values for these traits.…”

“…The first polymorphism examined was g.8398G>A previously demonstrated by Mitra et al (1995); however, we used the method designed by Chrenek et al (1998) in our evaluation. The second polymorphism (c.-1043A>G), located within the enhancer region, was detected according to Brym et al (2007). A total of 505 cows were genotyped for polymorphisms in the CYP11B1, SPP1 and PRL genes, while 500 cows were genotyped for the polymorphism in the PPARGC1A gene.…”

“…We hypothesise that this polymorphism is not causative but is in linkage with a functional polymorphism. Moreover, the PRLE polymorphism is known to influence transcription of the PRL gene (Brym et al, 2007).…”

Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo

“…Allelic variation in the structural or regulatory sequences of the prolactin gene would be of interest because of the possible direct and indirect effect on milk production. SNPs occurring within the prolactin gene may influence the chemical composition of milk or at least be an effective DNA marker of a sub-region of dairy cattle genome [8].…”

Genetic Polymorphisms of Some Bovine Lactogenic Hormones

“…In the 5' flanking region of the bovine prolactin gene, a distal regulatory element was found, which enhances the basal level of expression of the gene fivefold and functions independently of position and orientation. The postulated enhancer region extends from -1175 to -996 and displays considerable sequence similarity to equivalent regions in human and rat prolactin gene promoters (Brym et al, 2007).…”