Effect of nicotine in matrix mineralization by human bone marrow and Saos‐2 cells cultured on the surface of plasma‐sprayed titanium implants

Abstract

Smoking has an established negative impact in the clinical outcome of dental implants. This work analyses the response of human osteoblastic cells to nicotine, at the surface of plasma-sprayed commercial titanium implants. Human bone marrow (HBM) and Saos-2 cells, seeded on the surface of titanium implants and cultured in experimental conditions favoring osteoblastic differentiation, were exposed continuously to nicotine (0.0001 to 0.5 mg mL(-1)) and characterized for cell proliferation and function. Exposure of HBM cells resulted in increased cell proliferation, higher alkaline phosphatase (ALP) activity, and earlier onset of matrix mineralization at levels up to 0.2 mg mL(-1), an initial inhibitory effect in cell growth and functional activity followed by a recovery in the presence of 0.3 mg mL(-1) and a dose-dependent deleterious effect at higher levels. By contrast, exposure to nicotine did not affect cell proliferation of Saos-2 cells at levels up to 0.2 mg mL(-1), and caused only a small positive effect in ALP activity in the presence of 0.05 and 0.1 mg mL(-1); however, matrix mineralization by Saos-2 cells also occurred earlier in the cultures exposed to levels of nicotine up to 0.1 mg mL(-1). Higher concentrations caused dose-dependent inhibitory effects. Considering the high diffusion potential of nicotine, results suggest a local role of nicotine in modulating bone formation events at the implant surface.

Surface modification of three-dimensional Ca-P/PHBV nanocomposite scaffolds by physical entrapment of gelatin and its in vitro biological evaluation

“…However, it decreased from day 7 when cells differentiated to early osteoblasts and had a final maximum for the phenotype of the mature osteoblast. Similar trends in ALP activity have also been observed by others using different cell types or different biomaterials [32][33]. However, no significant difference was observed for ALP expression by the cells on Ca-P/PHBV scaffolds with or without surface modification.…”

Implications of tobacco smoking on the oral health of older adults

“…Increased levels of nicotine and free radicals influence the bone metabolism. They stimulate the resorptive process by reducing the recruitment of osteoblasts, their anchorage, adhesion, spreading, proliferation and differentiation . This subsequently decreases the production of collagen for extracellular matrix formation.…”

Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting

“…The EDS spectra showing a lack of calcium or phosphorus (Figure 5) in the matrix supports this observation. This was an expected result since optimal conditions for mineralization of SAOS-2 cell cultures (addition of beta glycerol phosphate ( β -GP)) (39–41) was not used. Experiments were specifically designed to evaluate cell proliferation and collagen synthesis since strength of calcified tissue depends in part on the molecular composition, structure and native organization of the collagenous matrix synthesized and laid down by the osteoblasts (18).…”