Effect of Nicotine on Hematology, Lipid Profile and Liver Enzymes in Adult Male Mice (Mus Musculus)

Abstract

This study was designed to observe the effects of nicotine on various hematological parameters, lipid and hepatic profile of adult male mice. A total of 80 mice were divided into two groups; control (n=40) and experimental (n=40). Subcutaneous injections of 1 mg/kg body weight of nicotine were given to the experimental and saline solution was given to the control group. The treatment lasted for 6 weeks after which blood was collected. Using hematological analyzer, hematological parameters were assessed whereas biochemical analysis was performed by chemistry analyzer. Statistical analysis was performed on SPSS. Comparison between the groups was done by T-test. The white blood cells, Platelet, Hematocrit, mean corpuscular volume, cholesterol, triglycerides and low density lipoprotein cholesterol were found significantly higher (p≤0.05) in an experimental group compared to control group while the significant decrease was observed in red blood cell count, hemoglobin, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, high density lipoprotein cholesterol and HDL-C/LDL-C ratio in the experimental group. Significant increase in the liver enzymes, alanine amino transferase aspartate aminotransferase and alkaline phosphatase were observed, whereas total bilirubin (mg/dl), albumin and total protein showed significant decrease in the experimental group. It is concluded that nicotine administration in mice resulted in deleterious effects on various hematological and biochemical parameters. All copyrights reserved to Nexus® academic publishers

Protective effects of quercetin and vitamin C against nicotine-induced toxicity in the blood of Wistar rats

“…This is in line with the study by Sharif et al (33), who have reported adverse nicotine effects on the lipid profile of adult male mice (Mus musculus). Once again, quercetin and vitamin C co-administration have shown their strong protective effects against nicotine toxicity by restoring lipid levels to near normal values (Table 2).…”

“…Since increased LPO disrupts the normal function or destroys erythrocyte membranes and causes a leakage of the cytoplasmic marker such as LDH (indicator of cell and tissue damage) into circulation (30), we expected to find elevated values of this parameter as well as enhanced activity of AST and ALT, based on earlier findings in blood and various organs (14,29,(31)(32)(33)(34). Indeed, treatment with nicotine significantly increased LDH levels and the activities of ALT, AST compared to control (Table 1).…”

Simultaneous Administration of Aqueous Extract of <i>Rosmarinus officinal</i> with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs

“…The present study demonstrated that nicotine treatment caused significant increases in the serum ALT, AST, ALP and Ɣ-GT activities and decreases in the serum total proteins, albumin and indicating impaired liver function. Similar results were also reported by Jang et al, [41] and Sharif et al, [42]. Fahim et al, [43] reported rise in both hepatic ALT and AST levels following i.p nicotine injection (1mg/Kg) for 3 weeks in mice.…”

Histopathological and Morphometric Evaluation in the Testis and Epididymis of Adult Rats Submitted To A Recovery Period after Treatment with Anabolic Steroid, Alcohol and/or Nicotine

“…According to the World Health Organization, smoking is the main cause of avoidable deaths and every year almost 6 million people die as consequence of tabagism. The nicotine acts as an endocrine disorganizer of male hormones [25,26], which promotes negative effects in the reproduction. The substance reduces the number of spermatozoa [27], increases the number of abnormal gametes and promotes changes in the testicular tissue [28][29][30].…”