Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells

Sajjanar, Basavaraj; Saxena, Shikha; Bisht, Deepika; Singh, Arvind Kumar; Reddy, G.B. Manjunatha; Singh, Vikram; Kumar, Satish

doi:10.1016/j.npep.2015.11.090

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…We also tested the binding of RABV-G-DFuLp to the RABV receptor using the 32-mer peptide derived from nAchR via surface plasmon resonance (SPR). Several reports have shown that RABV infection could be inhibited, via specific G/peptide interactions, by the torpedo peptide (T32) with the highest potency, by the bovine peptide (C32) with less efficiency, and by the human peptide (H32) with the least efficacy (Sajjanar et al, 2017(Sajjanar et al, , 2016. Falling in good accordance with these studies, RABV-G-DFuLp interacted most effectively with T32 (with an affinity of 1.7 mM in K D ) and less potently with C32 (3.1 mM in K D ) and only showed a marginal binding to H32 (>70 mM in K D ) ( Figure 1E).…”

Section: Preparation Of a Well-behaved Rabv-g Ecto-domain Proteinmentioning

confidence: 99%

Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody

Yang

Lin

et al. 2020

Cell Host & Microbe

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Section: Preparation Of a Well-behaved Rabv-g Ecto-domain Proteinmentioning

confidence: 99%

Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody

Yang

Lin

et al. 2020

Cell Host & Microbe

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“…The patient had neutralizing antibodies at the time of admitting to hospital and her therapeutic protocol for coma included midazolam and supplemental phenobarbital, and supportive treatment like ketamine, ribavirin and amantadine (Jackson 2016). Sajjanar et al (2016) reported that nicotinic acetylcholine receptor alpha 1 (nAChRa1) subunit peptides had specific affinity towards the RABV and these receptors may act as receptor decoy molecules results in inhibition of attachment of RABV to the neuronal cell receptors, which ultimately decrease viral replication. Torpedo peptide sequence (T-32) showed more antiviral action when compared to bovine, human and rat peptide sequences (Sajjanar et al 2016).…”

Section: Theraputic Approachesmentioning

confidence: 99%

“…Sajjanar et al (2016) reported that nicotinic acetylcholine receptor alpha 1 (nAChRa1) subunit peptides had specific affinity towards the RABV and these receptors may act as receptor decoy molecules results in inhibition of attachment of RABV to the neuronal cell receptors, which ultimately decrease viral replication. Torpedo peptide sequence (T-32) showed more antiviral action when compared to bovine, human and rat peptide sequences (Sajjanar et al 2016).…”

Section: Theraputic Approachesmentioning

confidence: 99%

Rabies – epidemiology, pathogenesis, public health concerns and advances in diagnosis and control: a comprehensive review

Singh

Cherian

et al. 2017

Veterinary Quarterly

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212

182

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Rabies is a zoonotic, fatal and progressive neurological infection caused by rabies virus of the genus Lyssavirus and family Rhabdoviridae. It affects all warm-blooded animals and the disease is prevalent throughout the world and endemic in many countries except in Islands like Australia and Antarctica. Over 60,000 peoples die every year due to rabies, while approximately 15 million people receive rabies post-exposure prophylaxis (PEP) annually. Bite of rabid animals and saliva of infected host are mainly responsible for transmission and wildlife like raccoons, skunks, bats and foxes are main reservoirs for rabies. The incubation period is highly variable from 2 weeks to 6 years (avg. 2-3 months). Though severe neurologic signs and fatal outcome, neuropathological lesions are relatively mild. Rabies virus exploits various mechanisms to evade the host immune responses. Being a major zoonosis, precise and rapid diagnosis is important for early treatment and effective prevention and control measures. Traditional rapid Seller's staining and histopathological methods are still in use for diagnosis of rabies. Direct immunofluoroscent test (dFAT) is gold standard test and most commonly recommended for diagnosis of rabies in fresh brain tissues of dogs by both OIE and WHO. Mouse inoculation test (MIT) and polymerase chain reaction (PCR) are superior and used for routine diagnosis. Vaccination with live attenuated or inactivated viruses, DNA and recombinant vaccines can be done in endemic areas. This review describes in detail about epidemiology, transmission, pathogenesis, advances in diagnosis, vaccination and therapeutic approaches along with appropriate prevention and control strategies.

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“…Of note, deficiency of T cell CAChT in mice promotes T cell exhaustion during viral infections. Therefore, the expression of CAChT and ACh in the immune cells is necessary to combat viral infections (Sajjanar et al 2016 ).…”

Section: Cholinergic System and Viral Infectionsmentioning

confidence: 99%

Cholinergic dysfunction in COVID-19: frantic search and hoping for the best

Nadwa

Al-Kuraishy

Al-Gareeb

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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A novel coronavirus known as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is a potential cause of acute respiratory infection called coronavirus disease 2019 (COVID-19). The binding of SARS-CoV-2 with angiotensin-converting enzyme 2 (ACE2) induces a series of inflammatory cellular events with cytopathic effects leading to cell injury and hyperinflammation. Severe SARS-CoV-2 infection may lead to dysautonomia and sympathetic storm due to dysfunction of the autonomic nervous system (ANS). Therefore, this review aimed to elucidate the critical role of the cholinergic system (CS) in SARS-CoV-2 infection. The CS forms a multi-faceted network performing diverse functions in the body due to its distribution in the neuronal and non-neuronal cells. Acetylcholine (ACh) acts on two main types of receptors which are nicotinic receptors (NRs) and muscarinic receptors (MRs). NRs induce T cell anergy with impairment of antigen-mediated signal transduction. Nicotine through activation of T cell NRs inhibits the expression and release of the pro-inflammatory cytokines. NRs play important anti-inflammatory effects while MRs promote inflammation by inducing the release of pro-inflammatory cytokines. SARS-CoV-2 infection can affect the morphological and functional stability of CS through the disruption of cholinergic receptors. SARS-CoV-2 spike protein is similar to neurotoxins, which can bind to nicotinic acetylcholine receptors (nAChR) in the ANS and brain. Therefore, cholinergic receptors mainly nAChR and related cholinergic agonists may affect the pathogenesis of SARS-CoV-2 infection. Cholinergic dysfunction in COVID-19 is due to dysregulation of nAChR by SARS-CoV-2 promoting the central sympathetic drive with the development of the sympathetic storm. As well, nAChR activators through interaction with diverse signaling pathways can reduce the risk of inflammatory disorders in COVID-19. In addition, nAChR activators may mitigate endothelial dysfunction (ED), oxidative stress (OS), and associated coagulopathy in COVID-19. Similarly, nAChR activators may improve OS, inflammatory changes, and cytokine storm in COVID-19. Therefore, nAChR activators like varenicline in virtue of its anti-inflammatory and anti-oxidant effects with direct anti-SARS-CoV-2 effect could be effective in the management of COVID-19.

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Effect of nicotinic acetylcholine receptor alpha 1 (nAChRα1) peptides on rabies virus infection in neuronal cells

Cited by 6 publications

References 36 publications

Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody

Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody

Rabies – epidemiology, pathogenesis, public health concerns and advances in diagnosis and control: a comprehensive review

Cholinergic dysfunction in COVID-19: frantic search and hoping for the best

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