Effect of nitrate and ammonium on the growth and protein concentration of Microcystis viridis Lemmermann (Cyanobacteria)

Rückert, Gabriela von; Giani, Alessandra

doi:10.1590/s0100-84042004000200011

Cited by 44 publications

(27 citation statements)

References 23 publications

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“…The cells of Microcystis spp. were able to utilize nitrate as a nitrogen source to form amino acids, chlorophyll and other organic compounds [5]. The decrease of nitrate in the medium macrophyte comprising the combination of F. globulosa and V. zizanoides without the addition of Microcystis in the fifteenth day reached about 99.94%, whereas the macrophyte combination in the medium with the addition of Microcystis was approximately 99.84%.…”

Section: Resultsmentioning

confidence: 97%

Control of Microcystis spp . Bloom By Combination of Indigenous Denitrifying Bacteria From Sutami Reservoir With Fimbristylis globulosa and Vetiveria zizanoides

Prahardika

Retnaningdyah²,

Suharjono³

2013

JTLS

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The purpose of this research is to know the ability of polyculture macrophyte ( Fimbristylis globulosa and Vetiveria zizanoides) and the combination of both with consortium of indigenous denitrifying bacteria from Sutami reservoir that was added by Microcystis spp. or not to reduce the concentration of nitrate, dissolved phosphate and the carrying capacity of Microcystis spp. The experiment was done in a medium filled up with Sutami reservoir water enriched with 16 ppm of nitrate and 0.4 ppm of phosphate. The denitrifying bacteria used in this research were DR-14, DU-27-1, DU-30-1, DU-30-2, TA-8 and DU-27-4 isolated from Sutami reservoir. The treatments were incubated within 15 days. Microcystis spp. abundance was calculated every day, but the measurement of the concentration of nitrate and dissolved phosphate was done every six days. The results showed that both treatment and the combination of both macrophytes with a consortium of denitrifying indigenous bacteria were added or not either Microcystis able to reduce nitrate at 99% and 93-99% orthophosphoric. The combination of macrophytes with denitrifying indigenous bacterial consortium from Sutami reservoir was able to inhibit the carrying capacity of Microcystis spp. highest up to 47.87%. They could also significantly reduce the abundance of Microcystis from 10 7 cells/mL in earlier days of the treatment into 0.35x10 4 cells/mL after fifteen days of incubation.

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Section: Resultsmentioning

confidence: 97%

Control of Microcystis spp . Bloom By Combination of Indigenous Denitrifying Bacteria From Sutami Reservoir With Fimbristylis globulosa and Vetiveria zizanoides

Prahardika

Retnaningdyah²,

Suharjono³

2013

JTLS

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“…These authors predicted dominance by eukaryotes when NO 3 -is the major N source, and cyanobacteria dominance would be strongly connected to NH 4 + availability, since this phylum seems to be able to grow faster in this nitrogen source (e.g. Herrero et al 2001;Rückert & Giani 2004). As observed in Marola Lake, other studies have demonstrated that NH 4 + may be the major DIN source in some eutrophic ecosystems (e.g.…”

Section: Discussionmentioning

confidence: 93%

The necessity of management in a lake of the Atlantic Forest biodiversity hotspot: nitrogen levels connected to a persistent bloom of Cylindrospermopsis raciborskii

2016

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Conservational studies of the threatened Atlantic Forest biome are frequently restricted to terrestrial ecosystems. We know little about the water bodies, specially considering that this biome covers the third largest system of lakes in Brazil. Some of these lakes are located inside the protected "Rio Doce State Park", but many others are found outside this reserve. Th ese external lakes are seldom studied, but understanding their response to human activities is essential for the conservation and the protection of the lakes inside the Park. We evaluated the eff ects of degradation in a lake outside the Park, which shows a constant bloom of the toxic invasive cyanobacteria Cylindrospermopsis raciborskii. Phytoplankton, climate and physico-chemical variables were assessed from 2011 to 2013 to evaluate which were the major determinants of the lake dynamics. Despite the seasonal changes, the lake was always eutrophic, and cyanobacteria, transparency and nutrients were the major indicators of water characteristics. Th e lake seems to be nitrogen-limited and cyanobacteria were negatively correlated with nitrogen levels, since the constantly dominant C. raciborskii is a superior competitor for N. We suggest that the monitoring of nitrogen levels is fundamental to establish management strategies to avoid harmful algae blooms in this Atlantic Forest lake.

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“…It is indicated that under nutrient limitation stress condition there is a negative correlation between growth rate and lipid content (Roleda et al, 2013). Inoculation of SH4 medium with seed cells grown in N8 put cells in an environment with low nitrogen but in form of ammonium that was easier to uptake (Von Ruckert and Giani, 2004). This situation increased biomass production because of the change in metabolism and improved specific growth rate from 0.0058/h to 0.0083/h.…”

Section: Resultsmentioning

confidence: 99%

Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium

Hamedi

Mahdavi

Gheshlaghi

2016

BRJ

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Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium. Biofuel Research Journal 10 (2016) HIGHLIGHTSUsing different media for inoculums preparation and microalgae production improved lipid and biomass productivities.When SH4 was selected for inoculation medium and N8 was selected for production medium, 130% increase in biomass productivity and 40% increase in lipid productivity was observed. Specific growth rate improved by differing inoculums preparation medium from production medium and changed from 0.0040/h to 0.0122/h. Improvement of biomass and lipid productivities is now one of the main concerns in commercialization of microalgae cultivation as a feedstock for algal biofuel production. Conventional photoautotrophic processes using well-studied and rich in oil strain of Chlorella vulgaris are not able to meet such demands. A new strategy of inoculating algae production medium with cells grown in a different medium from the production medium was proposed herein. More specifically, when SH4 was used as production medium and N8 was used as inoculation medium, biomass and lipid productivities increased by 2.33 folds and 1.44 folds, respectively, compared with when the production and inoculation media were the same, such as SH4. The findings of the present investigation showed that this cultivation scheme resulted in 52% increase in cell number and 54% increase in dry weight leading to improved productivities. Although by even considering this improvement, photoautotrophic cultivation of algae can hardly compete with the heterotrophic cultivation, the high cost of hydrocarbon supply required in large-scale heterotrophic processes marks the technique proposed in the present study as a promising approach for commercialization of algal biofuel production. ARTICLE INFO ABSTRACT © 2016 BRTeam. All rights reserved.Journal homepage: www.biofueljournal.com Hamedi et al. / Biofuel Research Journal 10 (2016) 410-416 Please cite this article as: Hamedi S., Mahdavi M.A., Gheshlaghi R. Improved lipid and biomass productivities in Chlorella vulgaris by differing inoculation medium from production medium.

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Effect of nitrate and ammonium on the growth and protein concentration of Microcystis viridis Lemmermann (Cyanobacteria)

Cited by 44 publications

References 23 publications

Control of Microcystis spp . Bloom By Combination of Indigenous Denitrifying Bacteria From Sutami Reservoir With Fimbristylis globulosa and Vetiveria zizanoides

Control of Microcystis spp . Bloom By Combination of Indigenous Denitrifying Bacteria From Sutami Reservoir With Fimbristylis globulosa and Vetiveria zizanoides

The necessity of management in a lake of the Atlantic Forest biodiversity hotspot: nitrogen levels connected to a persistent bloom of Cylindrospermopsis raciborskii

Improved lipid and biomass productivities in Chlorella vulgaris by differing the inoculation medium from the production medium

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