Effect of Nitric Oxide Generators on Ischemia-Reperfusion Injury and Histamine Release in Isolated Perfused Guinea Pig Heart

Masini, Emanuela; Gambassi, F.; Bianchi, S.; Mugnai, Laura; Lupini, M.; Pistelli, Alessandra; Mannaioni, P. F.

doi:10.1159/000235376

Cited by 16 publications

(17 citation statements)

References 3 publications

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“…It has been implicated in a number of physiological processes, including vasodilatation, smooth muscle relaxation and inhibition of platelet aggregation-adhesion. It also inhibits the neutrophil infiltration, release of histamine, degranulation of mast cells and accumulation of intracellular calcium [15][16][17]. The inhibition of mast cell degranulation, ischemia formation and platelet adhesion/ aggregation leads to a decrease in adhesion formation [4,[18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Effects of Diazeniumdiolates (NONOates) and Methylene Blue on the Reduction of Postoperative Adhesion in Rats

Çetin¹,

Duran²,

Demirköprülü³

et al. 2004

Gynecol Obstet Invest

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We investigated the effects of spermine NONOate (SPER/NO), diethylenetriamine NONOate (DETA/NO) and methylene blue for preventing postoperative adhesion in a rat uterine horn model. Before operations, rats were randomly assigned into 6 groups, each composed of 12 rats. These were the sham, control, normal saline, SPER/NO, DETA/NO and methylene blue groups. Each rat was anesthetized with ketamine hydrochloride (40 mg/kg i.v.). The abdominal wall was shaved; the surgical site was scrubbed with povidone iodine and rinsed with sterile saline 3 times before surgery. Under sterile conditions, a 3-cm vertical midline incision was made in all groups. In the sham group, we closed the abdominal wall without any procedure. In the other groups, a 2-cm segment of each uterine horn was injured in 10 spots on the antimesenteric surface using unipolar cautery. Before the final abdominal closure, no adjuvant therapy was administered intraperitoneally to the rats in the control group; 2 ml of normal saline solution, 1% methylene blue solution, SPER/NO (0.5 mg/ml) and DETA/NO (0.1 mg/ml) were instilled into the uterine horns of the rats in the respective groups. The incision was closed with a running 4-0 monofilament delayed absorbable suture in a single layer of muscle and fascia in a running pattern, excluding the peritoneum, and in the covering layer of skin in an interrupted pattern, in all groups. Two weeks after the surgery, all animals were killed, second laparotomies were performed, and the extent and severity of adhesions were determined by a blinded examiner. The adhesion scores of the sham group were significantly lower than those of the other groups (p < 0.05). In the methylene blue, SPER/NO and DETA/NO groups, adhesion scores were significantly lower than in the normal saline and control groups (p < 0.05). However, there were no significant differences related to the extent and severity scores of adhesions between the methylene blue, SPER/NO and DETA/NO groups (p > 0.05). This study showed that SPER/NO, DETA/NO and methylene blue administered at the end of surgery reduced the adhesion formation in a rat uterine horn model.

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Section: Discussionmentioning

confidence: 99%

Effects of Diazeniumdiolates (NONOates) and Methylene Blue on the Reduction of Postoperative Adhesion in Rats

Çetin¹,

Duran²,

Demirköprülü³

et al. 2004

Gynecol Obstet Invest

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“…NO modulates platelet adhesion and aggregation, leukocyte adhesion, endothelin generation and plasminogen activator enzymatic function [4,5,7,22]. It was suggested that neutrophil adherence is prevented by NO since it was shown that the use of NOS inhibitors (N·-t-BOC-ˆ-nitro-L-arginine) increased neutrophil adherence in cat mesentery [5,23].…”

Section: Discussionmentioning

confidence: 99%

“…The use of NO donors may be a reasonable therapeutic approach to reducing mast cell-dependent inflammation. It has been reported that NO have a beneficial role in the prevention of postischemic tissue injury in various organs including intestine, heart, lung and kidney [7][8][9][10]26]. The inhibition of mast cell degranulation, ischemia formation and platelet adhesion/aggregation leads to a decrease in adhesion formation [16,17,27,28].…”

Section: Discussionmentioning

confidence: 99%

“…The constitutive NOS has been isolated from vascular endothelial cells and neurons, and the inducible NOS from various cells including macrophages, smooth muscle cells, fibroblasts, mesenchymal cells and hepatocytes. Analogues of L-arginine such as Nˆ-nitro arginine methyl ester (L-NAME), N G -monomethyl L-arginine, and Nˆ-nitro-L-arginine inhibit the synthesis of NO [4][5][6][7]. NO has vasodilatory effects and inhibits platelet aggregation and neutrophil infiltration.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Nitric Oxide on Postoperative Adhesion Formation

Özden¹,

Bostancı²,

Sarioğlu

et al. 1999

Eur Surg Res

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Peritoneal adhesions continue to be a significant cause of postoperative complications. The purpose of the present study was to investigate the effect of nitric oxide in preventing postoperative adhesion formation in rats. Three randomized groups of Sprague-Dawley rats were subjected to a standardized lesion by cecal abrasion and parietal peritoneal defect. 0.9% NaCl (control, group 1), L-arginine (300 mg/kg, group 2) and Nω-nitro arginine methyl ester (L-NAME; 25 mg/kg, group 3) were administered intraperitoneally before abdominal closure and during 3 consecutive days after surgery. Two weeks after surgery, a relaparotomy was performed and the extent of adhesion formation was determined. In groups 1 and 3 heavy adhesions were detected. In the L-arginine group, adhesion formation was significantly less than in the other groups (p < 0.05). This study showed that L-arginine reduced adhesion formation.

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“…Several reports suggest that mast cell stabilizers such as disodium cromoglycate and lodoxamide tromethamine diminish acute I/R damage in both rat and rabbit hearts (19,27,37) possibly via inhibition of TNF-␣ release (11), whereas other investigators found that antigen-evoked "mast cell depletion" or lodoxamide tromethamine has no effect on I/R or hypoxia/reoxygenation injury in the rat heart (8, 36). The possible role of mast cell-derived histamine in acute ischemia as well as ischemic preconditioning has been discussed; however, several investigators have concluded that mast cell-derived histamine is not involved (3,22,37).In addition to their possible role in preconditioning and acute ischemic injury, mast cells have been proposed to play a role in postinfarction myocardial remodeling. Mast cell accumulation was observed in areas of collagen deposition in a canine model of I/R (9), in the subepicardium of infarcted and non-infarcted regions in rats (7), and in human hearts displaying ischemic as well as idiopathic cardiomyopathy (29).…”

mentioning

confidence: 99%

Cardiac function in hearts isolated from a rat model deficient in mast cells

Kennedy

Hauer‐Jensen²,

Joseph³

2005

American Journal of Physiology-Heart and Circulatory Physiology

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2004.-Several studies have examined the role of mast cells in the myocardial response to injury such as that caused by hypertension and ischemia-reperfusion. However, little is known about the influence of mast cells on normal myocardial structure and function. The present experiments examined cardiac function in Langendorff-perfused hearts isolated from 6-and 9-mo-old male mast cell-deficient (Ws/Ws) and mast cell-competent rats. A fluid-filled balloon catheter was used to measure left ventricular diastolic and systolic function at increasing preload volumes. At 6 mo of age, mast cell-deficient rats showed a slight cardiac hypertrophy (as monitored by heart weight and heart weight-to-body weight ratio) but no significant change in maximum observed systolic or diastolic function. In contrast, at 9 mo of age, the mast cell-deficient group showed no signs of hypertrophy but displayed a diastolic dysfunction characterized by decreased compliance without a significant decline in maximum observed basal ϪdP/dt max. There were no significant differences in maximum observed values for measures of systolic function (developed pressure and ϩdP/dtmax). In summary, the results of this study in adult rats suggest that mast cells influence cardiac function in the absence of injury and that observed differences between mast cell-competent and -deficient animals vary with age. Thus it is important to consider these "physiological" actions and resulting changes in function when studying effects of insult in mast cell-deficient models.compliance; diastolic dysfunction; Langendorff-perfused hearts MAST CELLS are part of the innate immune system that provides the first line of defense against tissue damage. In addition to their critical role in immunoglobulin E-dependent, histaminemediated hypersensitivity, mast cells release and modulate cytokines, growth factors, chemokines, proteases, and other mediators, which in turn regulate a vast array of important biological processes. Many mast cell mediators (e.g., histamine, heparin, tryptase, chymase, TNF-␣, and interleukin-4) are preformed and stored in large amounts in secretory granules, available for immediate release (21). Studies have demonstrated that mast cells reside in the myocardium even under physiological conditions, with their distribution along the coronary capillaries being more dense in the arteriolar section (30).Several studies have examined the potential role of mast cells in the myocardial response to injury. However, in many cases, the results of these studies have been contradictory. For example, numerous investigators have monitored the contribution of mast cells to preconditioning as well as ischemic injury. Parikh and Singh (27, 28) suggested that ischemia-and norepinephrine-induced preconditioning are mediated in part by degranulation of resident mast cells in the rat heart, whereas Wang et al. (37) reported that mast cell degranulation is not involved in ischemic cardiac preconditioning in rabbits. Similarly, the role of mast cells in the acute tissue damage...

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Effect of Nitric Oxide Generators on Ischemia-Reperfusion Injury and Histamine Release in Isolated Perfused Guinea Pig Heart

Cited by 16 publications

References 3 publications

Effects of Diazeniumdiolates (NONOates) and Methylene Blue on the Reduction of Postoperative Adhesion in Rats

Effects of Diazeniumdiolates (NONOates) and Methylene Blue on the Reduction of Postoperative Adhesion in Rats

Effect of Nitric Oxide on Postoperative Adhesion Formation

Cardiac function in hearts isolated from a rat model deficient in mast cells

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