Effect of nitric oxide in the differentiation of human monocytes to dendritic cells

Fernández-Ruiz, Verónica; González, Álvaro; López-Moratalla, N

doi:10.1016/j.imlet.2004.03.002

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…102 Additionally, NO influences DC maturation and differentiation and, hence, may have an impact on bacterial sampling and initiation of adaptive immune responses. 108 NO has been shown to modulate intestinal permeability and BT in different ways and directions. Nonspecific inhibition of NO Fig.…”

Section: Role Of Nitric Oxidementioning

confidence: 99%

Bacterial translocation (BT) in cirrhosis†

Wiest¹,

2005

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Section: Role Of Nitric Oxidementioning

confidence: 99%

Bacterial translocation (BT) in cirrhosis†

Wiest¹,

2005

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“…Monocytes were first isolated by plastic adherence and then were seeded at a concentration of 750,000 cells/ml and differentiated to immature dendritic cells (DC) with IL-4 1000 U/ml (ScheringPlough Research Institute, Kenilworth, NJ) plus GM-CSF 1000 U/ml (Leucomax, Basel, Switzerland) during 7 days, and matured with IFN-␥ 500 U/ml plus lipopolysaccharide (LPS) 10 g/ml (Sigma Aldrich, St. Louis, MO) for 48 hours according to the methodology described [26].…”

Section: Cell Culturementioning

confidence: 99%

Maternal antigen presenting cells are a source of plasmatic HLA-G during pregnancy: Longitudinal study during pregnancy

et al. 2007

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“…In human DCs, it has been demonstrated that exogenous NO affects their function partly via NO-guanylyl cyclase (NO-GC), a target of NO signaling (14)(15)(16). The endogenous production of NO can be catalyzed by three different NOS isoforms (inducible [iNOS], endothelial [eNOS], and neuronal [nNOS] NO synthase) that all operate in the immune system (17).…”

mentioning

confidence: 99%

Neuronal Nitric Oxide Synthase Modulates Maturation of Human Dendritic Cells

Adler¹,

Simon

Graulich³

et al. 2010

The Journal of Immunology

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Dendritic cells (DCs) are the most potent APCs of the immune system. Understanding the intercellular and intracellular signaling processes that lead to DC maturation is critical for determining how these cells initiate T cell-mediated immune processes. NO synthesized by the inducible NO synthase (iNOS) is important for the function of murine DCs. In our study, we investigated the regulation of the arginine/NO-system in human monocyte-derived DCs. Maturation of DCs induced by inflammatory cytokines (IL-1β, TNF, IL-6, and PGE2) resulted in a pronounced expression of neuronal NOS (nNOS) but only minimal levels of iNOS and endothelial NOS were detected in human mature DCs. In addition, reporter cell assays revealed the production of NO by mature DCs. Specific inhibitors of NOS (N-nitro-l-arginine methyl ester) or of the NO target guanylyl cyclase (H-(1,2,4)-oxadiazolo [4,3-a] quinoxalin-1-one) prevented DC maturation (shown by decreased expression of MHC class II, costimulatory and CD83 molecules and reduced IL-12 production) and preserved an immature phenotype, indicating an autocrine effect of nNOS-derived NO on human DC maturation. Notably, inhibitor-treated DCs were incapable of inducing efficient T cell responses after primary culture and generated an anergic T cell phenotype. In conclusion, our results suggest that, in the human system, nNOS-, but not iNOS-derived NO, plays an important regulatory role for the maturation of DCs and, thus, the induction of pronounced T cell responses.

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Effect of nitric oxide in the differentiation of human monocytes to dendritic cells

Cited by 20 publications

References 39 publications

Bacterial translocation (BT) in cirrhosis†

Bacterial translocation (BT) in cirrhosis†

Maternal antigen presenting cells are a source of plasmatic HLA-G during pregnancy: Longitudinal study during pregnancy

Neuronal Nitric Oxide Synthase Modulates Maturation of Human Dendritic Cells

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