Effect of Nitric Oxide on the Antifungal Activity of Oxidative Stress and Azoles Against Candida albicans

Li, Dedong; Yang, Cui; Liu, Ping; Wang, Yan; Sun, Yan

doi:10.1007/s12088-016-0580-x

Cited by 8 publications

(17 citation statements)

References 32 publications

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“…The role of NO in fungi has also been extensively studied. In Candida albicans, NO is involved in oxidative stress and azoles (31). In Trichoderma harzianum, NO can alleviate oxidative damage (32), and this effect was also demonstrated in P. eryngii var.…”

Section: Discussionmentioning

confidence: 89%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to P. ostreatus mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of aco could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase (aox) gene expression under heat stress, reduce the content of H 2 O 2 in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the aox gene in the heat stress response of mycelia. The results show that the colony diameter of the aox overexpression (OE-aox) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OEaox strains showed significantly enhanced tolerance to H 2 O 2 . In conclusion, this study demonstrates that NO can affect CA accumulation by regulating aco gene and ACO protein expression and that CA can induce aox gene expression and thereby be a response to heat stress. IMPORTANCE Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating aco. The results have enhanced our understanding of NO signaling pathways in P. ostreatus.

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Section: Discussionmentioning

confidence: 89%

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Hou

Zhao

Huang

et al. 2020

Appl Environ Microbiol

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“…The role of nNO in airway fungal diseases is still unclear. Nitric oxide has been reported to be associated with fungal clearance in hypodermis [ 19 ]. On the other hand, fungal pathogen was proved to inhibit the activity of iNOS, resulting in a dose-dependant NO suppression in an animal model [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Nasal nitric oxide in unilateral sinus disease

Tseng

Huang

et al. 2017

PLoS ONE

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Unilateral sinus disease (USD) can sometimes be difficult to accurately diagnose before surgery. The application of nasal nitric oxide (nNO) for USD diagnosis and its surgical outcome in USD has not been reported in the literature. We prospectively enrolled sixty-six USD patients who underwent endoscopic sinus surgery for fungal rhinosinusitis (n = 19), chronic rhinosinusitis (CRS) without nasal polyps (n = 13), CRS with nasal polyps (n = 12) and sinonasal mass lesions (n = 22). nNO levels were measured preoperatively and at three and six months postoperatively. Correlations between nNO levels and potential clinical parameters, type of disease, disease severity, and disease-related quality of life (QOL) were assessed. Unlike bilateral CRS, in USD, nNO levels did not correlate with disease severity or postoperative QOL improvements. Except for fungus group, there were no differences in nNO levels between lesion and non-lesion sides in all the other groups. nNO levels on both sides were significantly elevated six months postoperatively in all groups. Fungal rhinosinusitis patients had the lowest preoperative nNO levels, and a cutoff of 239.3 ppb had the best sensitivity (79.0%) and specificity (87.2%) for preoperative diagnosis. While preoperative nNO levels cannot serve as an alternative marker for disease severity of USD, they were lower in fungal rhinosinusitis patients than in other USD patients and may be useful for more accurate diagnosis prior to surgery.

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“…The situation in yeast is far less clear. Recently, in Candida albicans, a nitric oxide synthase pathway-like has been investigated [40], while in Saccharomyces cerevisiae, the flavoprotein Tah18 is involved in NO synthesis, and increases in this enzyme activity confer high-temperature stress tolerance to yeast [41]. TAH18 has an ortholog in C. albicans (orf19.2040), but its activity has yet to be confirmed.…”

Section: Discussionmentioning

confidence: 99%

Known Antimicrobials Versus Nortriptyline in Candida albicans: Repositioning an Old Drug for New Targets

Caldara

Marmiroli

2020

Microorganisms

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Candida albicans has the capacity to develop resistance to commonly used antimicrobials, and to solve this problem, drug repositioning and new drug combinations are being studied. Nortriptyline, a tricyclic antidepressant, was shown to have the capacity to inhibit biofilm and hyphae formation, along with the ability to efficiently kill cells in a mature biofilm. To use nortriptyline as a new antimicrobial, or in combination with known drugs to increase their actions, it is important to characterize in more detail the effects of this drug on the target species. In this study, the Candida albicans GRACE™ collection and a Haplo insufficiency profiling were employed to identify the potential targets of nortriptyline, and to classify, in a parallel screening with amphotericin B, caspofungin, and fluconazole, general multi-drug resistance genes. The results identified mutants that, during biofilm formation and upon treatment of a mature biofilm, are sensitive or tolerant to nortriptyline, or to general drug treatments. Gene ontology analysis recognized the categories of ribosome biogenesis and spliceosome as enriched upon treatment with the tricyclic antidepressant, while mutants in oxidative stress response and general stress response were commonly retrieved upon treatment with any other drug. The data presented suggest that nortriptyline can be considered a “new” antimicrobial drug with large potential for application to in vivo infection models.

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Effect of Nitric Oxide on the Antifungal Activity of Oxidative Stress and Azoles Against Candida albicans

Cited by 8 publications

References 32 publications

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Nasal nitric oxide in unilateral sinus disease

Known Antimicrobials Versus Nortriptyline in Candida albicans: Repositioning an Old Drug for New Targets

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