Effect of Nitrogen Addition on the Intra-Annual Leaf and Stem Traits and Their Relationships in Two Dominant Species in a Subtropical Forest

Abstract

Exploring the relationship between leaf and xylem growth and nitrogen (N) deposition on tree growth helps us better understand how N deposition affects tree growth, but relevant studies are still limited. We tested if leaf and xylem growth and their relationship were affected by N deposition across multiple stages of leaf development. We studied two dominant tree species (Castanopsis chinensis and Schima superba) in a subtropical forest in South China, monitoring their leaf and xylem growth traits under canopy N addition of 0 (CK) and 50 kg N ha−1 year−1 (CAN) and compared these traits and their relationships across different treatments, periods, and species. We found that CAN significantly increased C. chinensis’s leaf carbon (C) content, while the same treatment reduced leaf C content in S. superba. Specific leaf area (SLA), specific leaf weight (SLW), leaf N content, and C:N ratio showed more variation between species and within species across developmental stages than in response to different N addition treatments. Nitrogen addition also significantly increased the xylem width in both species, but the weekly xylem increment before July was notably higher in S. superba than in C. chinensis. Moreover, the leaf C content during early leaf development (ELD) following N addition was significantly and negatively correlated with the start date of xylogenesis in C. chinensis but had no significant effect on the onset of xylogenesis in S. superba. Additionally, regardless of species, SLA in ELD was negatively correlated with xylem width at the end of the early growing season (EW) under CK. However, this relationship became insignificant under CAN, suggesting that N addition alleviated the trade-off between SLA in ELD and EW. These results demonstrated that the relationship between leaf and xylem growth is influenced by both the leaf developmental stage and N availability. Traditional analyses of the leaf–xylem relationship, based solely on mature leaf traits, might oversimplify the effects of N deposition on tree growth.