Effect of nitrogen fertilisation on the amino acid digestibility of different triticale genotypes in caecectomised laying hens

Siegert, W.; Boguhn, J.; Maurer, Hans Peter; Weiß, Jochen; Zuber, Tobias; Möhring, Jens; Rodehutscord, M.

doi:10.1002/jsfa.7701

Cited by 17 publications

(9 citation statements)

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“…The concentrations of AA were determined according to a previously described method ( Rodehutscord et al., 2004 ) with modifications ( Siegert et al., 2017 ). In this assay, methionine and cysteine were determined as methionine sulphone and cysteic acid.…”

Section: Methodsmentioning

confidence: 99%

Phytase supplementation effects on amino acid digestibility depend on the protein source in the diet but are not related to InsP6 degradation in broiler chickens

et al. 2020

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The objective was to determine phytase effects on prececal amino acid ( AA ) digestibility and phytate (InsP 6 ) breakdown when different oilseed meals were used in broiler chicken diets. The study included 14 diets: a corn-soybean meal ( SBM ) basal diet and 6 diets that contained SBM, rapeseed meal ( RSM ), and sunflower meal ( SFM ) with 2 inclusion levels at the expense of corn starch (150 and 300 g/kg SBM or SFM, or 100 and 200 g/kg RSM). Each diet was mixed with or without a phytase supplement of 1,500 FTU/kg. Diets were provided to broilers for 5 D. Digesta from the posterior half of the ileum were collected on day 21. The average essential AA digestibility, calculated by a regression approach, without and with phytase was 84 and 85% (SBM), 74 and 77% (SFM), and 66 and 73% (RSM), respectively. In the diets, phytase effects on AA digestibility were lower owing to other protein sources also present in the diet, but significant. Prececal InsP 6 disappearance was significantly affected by interactions between oilseed meal, inclusion level, and phytase supplementation. Overall, prececal InsP 6 disappearance was higher in SBM diets (52%) than in SFM diets (38%) and intermediate in RSM diets (43%). Across diets, phytase supplementation effects on prececal InsP 6 degradation linearly increased with the InsP 6 concentration of the diet up to 12 g/kg DM. The only exception from linearity was the diet with the high inclusion of SFM, which contained 15.9 g InsP 6 /kg DM. In the ileal content, the concentration of myo -inositol was significantly increased by phytase supplementation, and this effect was highest in the diets that contained SBM as the only oilseed meal. Concentrations of lower inositol phosphates were increased by phytase supplementation, and this effect was most remarkable for Ins(1,2,3,4)P 4 and inositol tetrakisphosphates. The study showed that phytase effects on AA digestibility varied among the 3 tested oilseed meals, but these differences were not detectable in the diets containing these meals. Although phytase effects on ileal content of InsP 6 and its degradation products were substantial, they were not related to the effects on AA digestibility.

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Section: Methodsmentioning

confidence: 99%

Phytase supplementation effects on amino acid digestibility depend on the protein source in the diet but are not related to InsP6 degradation in broiler chickens

et al. 2020

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“…Amino acid analysis was conducted according to the method described by Siegert et al. (2017). Samples were hydrolyzed in acidic conditions at 113°C for 24 h after oxidation in an ice bath.…”

Section: Methodsmentioning

confidence: 99%

Prececal amino acid digestibility and phytate degradation in broiler chickens when using different oilseed meals, phytase and protease supplements in the feed

et al. 2019

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The purpose of this study was to investigate the effects of phytase and protease supplementation on prececal (pc) amino acid (AA) digestibility, phytate (InsP6) degradation, and MEn concentration in diets using 3 oilseed meals as main protein sources in broiler chicken feed. The broiler chicken diets, which lacked mineral phosphorus, contained either soybean meal (SBM), SBM and rapeseed meal (SBM/RSM), or SBM and sunflower meal (SBM/SFM) as main protein sources. Diets were not supplemented with enzymes or supplemented with 1,500 or 3,000 FTU phytase/kg, or with 1,600 mg protease/kg. For diets containing SBM as the main protein source, the effects of phytase supplementation with and without monocalcium phosphate were also investigated. Data were obtained during 2 subsequent runs from days 14 to 22 and from days 23 to 31. Each diet was tested using 8 replicates with 4 replicates per run. For pc AA digestibility, no significant interactions were observed between main protein sources, enzyme supplementation, or addition of monocalcium phosphate except for Cys. Supplementation of 1,500 FTU phytase/kg increased pc digestibility of all AA. No differences in pc AA digestibility were observed between 1,500 and 3,000 FTU phytase/kg supplementation treatments. Prececal disappearance of InsP6 and pc P digestibility were greater in the high phytase supplementation treatment. Protease supplementation increased pc digestibility of all AA except for Cys when SBM/RSM was the main protein source. Supplementation of protease and 3,000 FTU phytase/kg increased MEn concentrations. The effect of phytase on pc AA digestibility was fully expressed at a lower supplementation level than needed for a maximized pc InsP6 disappearance and MEn concentration.

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“…Gross energy was determined by bomb calorimetry 26 . AA were analysed as described previously 18 with minor modifications 27 . Gly in excreta is not presented because Gly formation from uric acid occurs during sample hydrolysis 28 .…”

Section: Methodsmentioning

confidence: 99%

“…26 AA were analysed as described previously 18 with minor modifications. 27 Gly in excreta is not presented because Gly formation from uric acid occurs during sample hydrolysis. 28 Asp and Asn are presented together as Asx, and Glu and Gln are presented together as Glx because Asn is converted to Asp and Gln is converted to Glu during acid hydrolysis.…”

Section: Sample Preparation and Chemical Analysesmentioning

confidence: 99%

Amino acid digestibility and metabolisable energy of spring and winter faba beans grown on two sites and effects of dehulling in caecectomised laying hens

et al. 2021

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BACKGROUND: The variation in amino acid (AA) digestibility and metabolisable energy (ME N ) in four spring and four winter faba bean genotypes differing in vicine/convicine (V/C) concentrations grown on two sites was investigated in caecectomised LSL-Classic laying hens. Effects of dehulling one faba bean genotype were also examined. Diets containing one out of 17 faba bean variants each and a basal diet were fed to ten caecectomised laying hens in a row-column design to achieve five replicates per diet.RESULTS: Ranges and levels of digestibility of the hulled variants differed widely among AA with the lowest and highest range determined for Arg (90-93%) and Cys (−12-65%), respectively. ME N ranged between 10.3 and 12.3 MJ kg −1 dry matter. Lower ME N and digestibility of Cys, Glx, Phe, Pro, Tyr, and Val (P < 0.050) was determined for the winter genotypes grown in Nimtitz compared to the other variants. Digestibility of Ser was lower for the spring than for the winter genotypes (P < 0.050). Negative correlations with AA digestibility were determined for phytate, but not for tannin and V/C concentrations (P < 0.050). Negative correlations between tannin fractions and ME N were weak (P = 0.082-0.099). Dehulling increased ME N by 1.8 MJ kg −1 dry matter and raised the digestibility of Pro, His, and Glx (P < 0.050). CONCLUSIONS:The results indicated that the digestible AA and ME N supply of laying hens was increased by using low phytate faba beans while breeding for low V/C genotypes did not affect AA digestibility or ME N . Dehulling increased ME N and the digestibility of some AA.

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Effect of nitrogen fertilisation on the amino acid digestibility of different triticale genotypes in caecectomised laying hens

Abstract: The results indicated that the accuracy of the digestible AA supply for hen feeding might benefit from considering fertilisation and genotype-specific digestibility data in feed formulation. © 2016 Society of Chemical Industry.

Cited by 17 publications

References 20 publications

Phytase supplementation effects on amino acid digestibility depend on the protein source in the diet but are not related to InsP6 degradation in broiler chickens

Phytase supplementation effects on amino acid digestibility depend on the protein source in the diet but are not related to InsP6 degradation in broiler chickens

Prececal amino acid digestibility and phytate degradation in broiler chickens when using different oilseed meals, phytase and protease supplements in the feed

Amino acid digestibility and metabolisable energy of spring and winter faba beans grown on two sites and effects of dehulling in caecectomised laying hens

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