Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Kalil, Mohd Sahaid; Alshiyab, Hisham; Yusoff, Wan Mohtar Wan

doi:10.3844/ajbbsp.2008.393.401

Cited by 51 publications

(29 citation statements)

References 25 publications

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“…These result showed that digester A-C had C/N ratio about 31.2, produced total quantity of biogas higher that of digester D and E which similar to the previously report by Mohd et al (2008).…”

Section: Characteristics Of Raw Materialssupporting

confidence: 90%

The Effect of Natural Water With Cow Dung and Agricultural Waste Ratio on Biogas Production From Anaerobic Co-Digestion

NaponKeanoi¹

2013

American Journal of Environmental Sciences

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Global warming caused by energy generation from fossil fuel has accelerated the deployment of renewable fuels such as biogas. In this study, batch fermentation (5L) was studied to observe the effect of natural water, cow dung, rice straw and water hyacinth ratio on the biogas and methane production at ambient temperature (31°C), 7.1-7.4 of pH for 52 days. The five types of co-digestion were 2:1:1:1 (digester A), 1:1:1:1 (digester B), 0.5:1:1:1 (digester C), 1:1:1:0 (digester D) and 1:1:0:1 (digester E), respectively. The result showed that the biogas production increased progressively with amount of natural water to raw material. The maximum biogas production and methane concentration was 1650 mL/days and 61.47% was obtained at substrate mixture 2:1:1:1 (digester A), which there are suitable of C:N ratio at 31.1:1. Natural water, cow dung, rice straw and water hyacinth was mixed for biogas production, have been found to improve highest biogas production compared to those of without rice straw and water hyacinth. Therefore, the mixing natural water, cow dung, rice straw and water hyacinth can improve both biogas production and content of methane in biogas.

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Section: Characteristics Of Raw Materialssupporting

confidence: 90%

The Effect of Natural Water With Cow Dung and Agricultural Waste Ratio on Biogas Production From Anaerobic Co-Digestion

NaponKeanoi¹

2013

American Journal of Environmental Sciences

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“…Nitrogen source was generally required for enzymes, proteins, and nucleic acid synthesis—all crucial components for generation of new cells (Rughoonundun, Mohee, & Holtzapple, ). Nitrogen source could be either organic (such as yeast extract, tryptone, and peptone) or inorganic (e.g., ammonium sulfate [AMS], ammonium nitrate, and ammonium chloride) forms (Kalil, Alshiyab, & Yusoff, ). Our previous study indicated that 1 kg of C‐grade fresh longan fruit contained 25.8 ± 0.3 g sucrose, 13.9 ± 0.5 g glucose, 11.9 ± 0.3 g fructose, 51.6 ± 0.3 g total sugar, and only 0.021 ± 0.004 g of nitrogen (Nunta et al, ).…”

Section: Introductionmentioning

confidence: 99%

Batch and continuous cultivation processes of Candida tropicalis TISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio

Nunta

Techapun

Jantanasakulwong

et al. 2019

J Food Process Engineering

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The carbon to nitrogen (C/N) molar ratio of 21.88 ± 0.20 in fresh juice from C‐grade longan (FLJ‐C) supplemented with ammonium sulfate (AMS) was optimal for cultivation of Candida tropicalis TISTR 5306 producing ethanol concentration of 24.0 ± 1.1 g/L and specific pyruvate decarboxylase (PDC) activity of 0.138 ± 0.001 U/mg protein. The highest ethanol concentration in a 10 L continuous process (34.3 ± 0.5 g/L, dilution rate [D] = 0.0492 hr−1) was significantly higher (p ≤ .05) than that in 100 L batch process (13.2 ± 0.2 g/L at 108 hr). The highest volumetric PDC activities of 0.081 ± 0.001 U/ml (D = 0.0070 hr−1) and 0.107 ± 0.001 U/ml (48 hr) were attained for continuous and batch processes, respectively. The affinity constants and growth‐related weighting factors on sucrose and monosaccharides (glucose and fructose with the same values) utilization were (123 ± 1 g/L, 0.14 ± 0.01) and (34.1 ± 1.5 g/L, 0.43 ± 0.01) with MS and R2 of 92.2 and .9552, respectively. Practical applications FLJ‐C is an example of agricultural produces with relatively high production quantity (50,000 t/year in Thailand) and high sugars content but low marketing demands due to the smallest size of pulp. The complex nitrogen source contains costly components (yeast extract—78.3 USD/kg, malt extract—148 USD/kg, and peptone—151 USD/kg) which can be replaced with an inexpensive AMS (1.05 USD/kg). Further consideration of C/N molar ratio in FLJ‐C can optimize growth, ethanol (alternative biofuel/industrial solvent) production, and specific PDC (biocatalyst for phenylacetylcarbinol—PAC, anti‐asthmatic and nasal decongestant precursors) activity of C. tropicalis TISTR 5306 whole cells which can be used as a case study for other related yeasts. Batch and continuous production at 100 L and 10 L also illustrate practical utilization of FLJ‐C with AMS supplement at larger scales for development of subsequent zero waste process.

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“…It has also been demonstrated that the C/N ratio has a great influence on growth and metabolite production for a variety of microorganisms [27,28].…”

Section: Introductionmentioning

confidence: 99%

Tuning of the Carbon-to-Nitrogen Ratio for the Production of l-Arginine by Escherichia coli

Ginesy

Rusanova-Naydenova

2017

Fermentation

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L-arginine, an amino acid with a growing range of applications within the pharmaceutical, cosmetic, food, and agricultural industries, can be produced by microbial fermentation. Although it is the most nitrogen-rich amino acid, reports on the nitrogen supply for its fermentation are scarce. In this study, the nitrogen supply for the production of L-arginine by a genetically modified Escherichia coli strain was optimised in bioreactors. Different nitrogen sources were screened and ammonia solution, ammonium sulphate, ammonium phosphate dibasic, and ammonium chloride were the most favourable nitrogen sources for L-arginine synthesis. The key role of the C/N ratio for L-arginine production was demonstrated for the first time. The optimal C/N molar ratio to maximise L-arginine production while minimising nitrogen waste was found to be 6, yielding approximately 2.25 g/L of L-arginine from 15 g/L glucose with a productivity of around 0.11 g/L/h. Glucose and ammonium ion were simultaneously utilized, showing that this ratio provided a well-balanced equilibrium between carbon and nitrogen metabolisms.

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Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Cited by 51 publications

References 25 publications

The Effect of Natural Water With Cow Dung and Agricultural Waste Ratio on Biogas Production From Anaerobic Co-Digestion

The Effect of Natural Water With Cow Dung and Agricultural Waste Ratio on Biogas Production From Anaerobic Co-Digestion

Batch and continuous cultivation processes of Candida tropicalis TISTR 5306 for ethanol and pyruvate decarboxylase production in fresh longan juice with optimal carbon to nitrogen molar ratio

Tuning of the Carbon-to-Nitrogen Ratio for the Production of l-Arginine by Escherichia coli

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