Humic substances (HS) are vital to
soil fertility and carbon sequestration.
Using multiple cross-polarization/magic-angle spinning (multiCP/MAS)
NMR combined with dipolar dephasing, we quantitatively characterized
humic fractions, i.e., fulvic acid (FA), humic acid (HA), and humin
(HM), isolated from two representative soils (upland and paddy soils)
in China under six long-term (>20 years) fertilizer treatments.
Results
indicate that each humic fraction showed chemical distinction between
the upland and paddy soils, especially with much greater aromaticity
of upland HMs than of paddy HMs. Fertilizer treatment exerted greater
influence on chemical natures of upland HS than of paddy HS, although
the effect was less than that of soil type. Organic manure application
especially decreased the percentages of aromatic C in the upland HAs
and HMs compared with the control. We concluded that humic fractions
responded in chemical nature to environmental conditions, i.e., soil
type/cropping system/soil aeration and fertilizer treatments.