Effect of non-linear strain stiffening in eDAH and unjamming

Xie, Xiaofan; Sauer, Frank; Grosser, Steffen; Lippoldt, Jürgen; Warmt, Enrico; Das, Amit; Bi, Dapeng; Fuhs, Thomas; Käs, Josef A.

doi:10.1039/d3sm00630a

Cited by 4 publications

(2 citation statements)

References 72 publications

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“…54 Recent experimental work measuring stiffness of epithelial packings with atomic force microscopy (AFM) has shown that reducing sphericity indeed results in stiffer tissue. 60…”

Section: Resultsmentioning

confidence: 99%

“…For instance, comparison of size and sphericity shows that sphericity can become important, with more spherical cells reducing the stress compared to elongated cells. 60 In metabolic disease, the reduced proliferation of cells and their enlargement can lead to more disorganized geometry, overall reducing sphericity, 13 which would lead to an increase in stiffness, counteracting to a small extent the effect of the larger cell size. Moreover, mean size and mean sphericity are, by themselves, not fully predictive of the biomechanics of the tissue: spread of the distribution of size and sphericity is also crucial (see Fig.…”

Section: Discussionmentioning

confidence: 99%

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Geometry of adipocyte packing in subcutaneous tissue contributes to nonlinear tissue properties captured through a Gaussian process surrogate model

Barsimantov Mandel,

Solorio,

Tepole

2024

Soft Matter

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“…54 Recent experimental work measuring stiffness of epithelial packings with atomic force microscopy (AFM) has shown that reducing sphericity indeed results in stiffer tissue. 60…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Geometry of adipocyte packing in subcutaneous tissue contributes to nonlinear tissue properties captured through a Gaussian process surrogate model

Barsimantov Mandel,

Solorio,

Tepole

2024

Soft Matter

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Linking Metastatic Potential and Viscoelastic Properties of Breast Cancer Spheroids via Dynamic Compression and Relaxation in Microfluidics

Tavasso,

Bordoloi,

Tanré

et al. 2024

Adv Healthcare Materials

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The growth and invasion of solid tumors are associated with changes in their viscoelastic properties, influenced by both internal cellular factors and physical forces in the tumor microenvironment. Due to the lack of a comprehensive investigation of tumor tissue viscoelasticity, the relationship between such physical properties and cancer malignancy remains poorly understood. Here, the viscoelastic properties of breast cancer spheroids, 3D (in vitro) tumor models, are studied in relation to their metastatic potentials by imposing controlled, dynamic compression within a microfluidic constriction, and subsequently monitoring the relaxation of the imposed deformation. By adopting a modified Maxwell model to extract viscoelastic properties from the compression data, the benign (MCF‐10A) spheroids are found to have higher bulk elastic modulus and viscosity compared to malignant spheroids (MCF‐7 and MDA‐MB‐231). The relaxation is characterized by two timescales, captured by a double exponential fitting function, which reveals a similar fast rebound for MCF‐7 and MCF‐10A. Both the malignant spheroids exhibit similar long‐term relaxation and display residual deformation. However, they differ significantly in morphology, particularly in intercellular movements. These differences between malignant spheroids are demonstrated to be linked to their cytoskeletal organization, by microscopic imaging of F‐actin within the spheroids, together with cell‐cell adhesion strength.

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Different contractility modes control cell escape from multicellular spheroids and tumor explants

Blauth,

Grosser,

Sauer

et al. 2024

APL Bioengineering

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Cells can adapt their active contractile properties to switch between dynamical migratory states and static homeostasis. Collective tissue surface tension, generated among others by the cortical contractility of single cells, can keep cell clusters compact, while a more bipolar, anisotropic contractility is predominantly used by mesenchymal cells to pull themselves into the extracellular matrix (ECM). Here, we investigate how these two contractility modes relate to cancer cell escape into the ECM. We compare multicellular spheroids from a panel of breast cancer cell lines with primary tumor explants from breast and cervical cancer patients by measuring matrix contraction and cellular spreading into ECM mimicking collagen matrices. Our results in spheroids suggest that tumor aggressiveness is associated with elevated contractile traction and reduced active tissue surface tension, allowing cancer cell escape. We show that it is not a binary switch but rather the interplay between these two contractility modes that is essential during this process. We provide further evidence in patient-derived tumor explants that these two contractility modes impact cancer cells' ability to leave cell clusters within a primary tumor. Our results indicate that cellular contractility is an essential factor during the formation of metastases and thus may be suitable as a prognostic criterion for the assessment of tumor aggressiveness.

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Effect of non-linear strain stiffening in eDAH and unjamming

Abstract: In cell clusters, the prominent factors at play encompass contractility-based enhanced tissue surface tension, and cell unjamming transition. The former effect pertains to boundary effect, while the latter constitutes a...

Cited by 4 publications

References 72 publications

Geometry of adipocyte packing in subcutaneous tissue contributes to nonlinear tissue properties captured through a Gaussian process surrogate model

Geometry of adipocyte packing in subcutaneous tissue contributes to nonlinear tissue properties captured through a Gaussian process surrogate model

Linking Metastatic Potential and Viscoelastic Properties of Breast Cancer Spheroids via Dynamic Compression and Relaxation in Microfluidics

Different contractility modes control cell escape from multicellular spheroids and tumor explants

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