Effect of notoginsenoside R1 on the synthesis of tissue-type plasminogen activator and plasminogen activator inhibitor-1 in cultured human umbilical vein endothelial cells.

Abstract

Among other Chinese herb drugs, Panax notoginseng is used to treat cardiovascular diseases. To elucidate any possible effects of this drug on the hemostatic system in vitro, we analyzed the influence of one of its major active constituents on fibrinolytic parameters of cultured human umbilical vein endothelial cells (HUVECs). When confluent cultures of HUVECs (passages 2 to 3) were conditioned with purified notoginsenoside Rl (NR1), a dose-(0.01 to 100 fig NRl/mL) and time-dependent increase in tissue-type plasminogen activator (TPA) synthesis was observed, which was significant from 0.1 jig NRl/mL and from 6 hours of incubation with 100 /xg NRl/mL on. TPA antigen increased from 3.9 ±0.2 ng per 1$ cells per 24 hours to 8.0±0.5 ng per 10 5 cells per 24 hours on addition of 100 /ig NRl/mL. In contrast, no change in urokinase-type plasminogen activator and plasminogen activator inhibitor-1 (PAI-1) antigen synthesis was seen. There was also no effect of NR1 on PAI-1 deposition in the extracellular matrix. As judged from fibrin autography and reverse fibrin

Suppressive effect of AIF, a water extract from three herbs, on collagen-induced arthritis in mice

“…In this regard, it is interesting that P. notoginseng which we found to inhibit the secretion of TNF-a has been used to treat various vascular diseases and cardiac pain. Zhang et al reported that notoginsenoside R1 from P. notoginseng has a fibrinolytic activity by increasing tissue-type plasminogen activator and decreasing plasminogen activator inhibitor-1 [24]. More recently, the decrease of total cholesterol, tri-glycerides and fibrinogen in blood by the treatment of P. notoginseng pulverized root has been reported [25].…”

The effects of notoginsenoside R1 on the intestinal absorption of geniposide by the everted rat gut sac model

“…1). It has exhibited various health promotion activities, including being neuroprotective (Gu et al, 2009), hepatic protective (Chen et al, 2008), cardiovascular protective (Zhang et al, 1994), and exhibiting immune stimulation effects.…”

Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction

“…It is also contained in several famous Chinese traditional formula, such as "Yunnan Baiyao" which is used for treatment of injury induced trauma and bleeding, and "Pian Zi Huang" used to treat hepatitis. Current pharmacological studies revealed that P. notoginseng and its saponins possess anticarcinogenic [4,5] and hepatoprotective [6] properties, as well as protective effects on cardiovascular and cerebrovascular diseases [7][8][9].…”