Effect of novel Lactobacillus paracaesi microcapsule on growth performance, gut health and microbiome community of broiler chickens

Gyawali, Ishwari; Zeng, Yuanyuan; Zhou, Jingjing; Li, Jincheng; Wu, Ting; Shu, Gang; Jiang, Qingyan; Zhu, Canjun

doi:10.1016/j.psj.2022.101912

Cited by 18 publications

(17 citation statements)

References 67 publications

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“…Probiotic bacteria, on the other hand, help preserve the integrity of the epithelium [ 47 , 48 , 49 ], which stimulates the absorption of nutrients and ultimately leads to a superior growth performance [ 50 , 51 ]. L. fermentum , as many other Lactobacillus species, has proved useful for ameliorating intestinal health of broiler chickens [ 24 , 52 , 53 , 54 ]. Indeed, this probiotic species improved the height of villi in duodenal and jejunal sections, but it did not influence crypt depth.…”

Section: Discussionmentioning

confidence: 99%

Effects of Lactobacillus fermentum Administration on Intestinal Morphometry and Antibody Serum Levels in Salmonella-Infantis-Challenged Chickens

et al. 2023

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There are no studies reporting the effects of Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) on intestinal architecture and immunoglobulin serum levels in chickens. Here, we measured these parameters and hypothesized whether probiotic administration could modulate the observed outcomes. Two-hundred 1-day-old COBB 500 male chicks were allocated into four groups: (I) the control, (II) the group treated with L. fermentum, (III) the group exposed to S. Infantis, and (IV) the group inoculated with both bacteria. At 11 days post infection, blood was gathered from animals which were then euthanized, and samples from the small intestine were collected. Intestinal conditions, as well as IgA and IgM serum levels, were assessed. S. Infantis reduced villus-height-to-crypt-depth (VH:CD) ratios in duodenal, jejunal, and ileal sections compared to control conditions, although no differences were found regarding the number of goblet cells, muc-2 expression, and immunoglobulin concentration. L. fermentum improved intestinal measurements compared to the control; this effect was also evidenced in birds infected with S. Infantis. IgM serum levels augmented in response to the probiotic in infected animals. Certainly, the application of L. fermentum elicited positive outcomes in S. Infantis-challenged chickens and thus must be considered for developing novel treatments designed to reduce unwanted infections.

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Section: Discussionmentioning

confidence: 99%

Effects of Lactobacillus fermentum Administration on Intestinal Morphometry and Antibody Serum Levels in Salmonella-Infantis-Challenged Chickens

et al. 2023

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“…This is because the microbial community of the gastrointestinal tract plays a role in extracting energy from nutrients as well as harboring potential pathogenic organisms such as Campylobacter , Salmonella enterica , Escherichia coli , and Clostridium perfringens that can colonize and cause illness to the avian host and humans ( Clavijo and Flórez, 2018 ). The current body of literature includes many microbiome analyses regarding the effect of probiotics ( Gao et al, 2017 ; Rodrigues et al, 2020 ; Gyawali et al, 2022 ) or pathogen exposure ( Park et al, 2017 ; Latorre et al, 2018 ; Joat et al, 2021 ) on the broiler microbiome. However, it should be noted that many of these single-facet studies utilize conventional, fast-growing broilers such as the Ross or Cobb breeds.…”

Section: Discussionmentioning

confidence: 99%

Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers

et al. 2022

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Despite the negative impacts of Salmonella intestinal colonization on human health, Salmonella is a natural colonizer of the gastrointestinal tract and is not overtly pathogenic to the avian host. It is of interest to understand the impacts and colonization rates of Salmonella across selected genetic lines such as slow-growing (SG) and conventional (CONV) broilers. The objective of this study was to characterize the relationship between Salmonella enterica serovar Typhimurium challenge and selected broiler genetic lines on the ileal and cecal microbiome. Male chicks of two broiler breeds (n = 156/breed) were cohoused in an open floor pen until day 7. On day 13, the chicks were then separated into 12 isolators per breed (4 rooms, 6 isolators/room, 11 chicks/isolator). On day 14, chicks in the 12 treatment isolators (6 isolators/breed, 108 total) were challenged with Salmonella Typhimurium (ST) (1 × 108 CFU/ml) via oral gavage while the remaining chicks (n = 108) were given an oral gavage of sterile tryptic soy broth control (C). Ileal and cecal contents were collected on day 7 from 24 chicks of each breed, and on days 13, 17, 21, and 24 from two chicks per isolator. Samples underwent DNA extraction and PCR amplification to obtain 16S rRNA amplicons that were sequenced with Illumina MiSeq. Salmonella Typhimurium colonization in the cecum was not different in the two broiler breeds. The main effect of breed had the greatest impact on the ileum microbiota of broilers 7 days of age where SG broilers had significantly lower diversity and richness compared to CONV broilers (p < 0.05). Salmonella Typhimurium challenge consistently caused a change in beta diversity. Regardless of day or intestinal location, challenged broilers had many amplicon sequence variants (ASVs) with decreased abundance of likely beneficial bacteria such as Mollicutes RF39, Shuttleworthia, Flavonifractor, and Oscillibacter compared to broilers that were unchallenged with Salmonella Typhimurium (p < 0.05). Additionally, there was a difference in the timing of when the microbiota alpha and beta diversity of each breed responded to Salmonella Typhimurium challenge. Thus, both broiler breed and Salmonella Typhimurium can impact the intestinal microbiota.

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“…Probiotics, defined as “live microorganisms which, when administered in adequate amounts, confer a health benefit on the host” ( FAO/WHO, 2001 ) continue to receive growing interest as an alternative to AGP in poultry production as a result of its immunomodulating properties ( Pender et al., 2016 ; Abd El-Ghany et al., 2022 ). Evidence abounds in the literature of the potential of probiotics to improve the growth performance of poultry ( Torres-Rodriguez et al., 2007 ; Sen et al., 2012 ; Bai et al., 2013 ), improve nutrient digestibility ( Mountzouris et al., 2010 ; Nawaz et al., 2016 ; Opoola et al., 2021 ), improve gut health ( Oladokun et al., 2021a ; Zeng et al., 2021 ; Gyawali et al., 2022 ), stimulate immunity ( Nawaz et al., 2016 ; Abd El-Ghany et al., 2022 ; Hedayati et al., 2022 ) and positively modulate gut microbiota profile ( Mountzouris et al., 2007a , 2010 ; Hedayati et al., 2022 ). Popular probiotic strains utilized in poultry include Lactobacillus, Bifidobacterium, Enterococcus , and Bacillus ( Bajagai et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

The effect of Bacillus subtilis and its delivery route on hatch and growth performance, blood biochemistry, immune status, gut morphology, and microbiota of broiler chickens

Oladokun¹,

Adewole²

2023

Poultry Science

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Effect of novel Lactobacillus paracaesi microcapsule on growth performance, gut health and microbiome community of broiler chickens

Cited by 18 publications

References 67 publications

Effects of Lactobacillus fermentum Administration on Intestinal Morphometry and Antibody Serum Levels in Salmonella-Infantis-Challenged Chickens

Effects of Lactobacillus fermentum Administration on Intestinal Morphometry and Antibody Serum Levels in Salmonella-Infantis-Challenged Chickens

Ileal and cecal microbiota response to Salmonella Typhimurium challenge in conventional and slow-growing broilers

The effect of Bacillus subtilis and its delivery route on hatch and growth performance, blood biochemistry, immune status, gut morphology, and microbiota of broiler chickens

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