Effect of nucleases on the cellular internalization of fluorescent labeled DNA-functionalized single-walled carbon nanotubes

Moon, Hye Kyung; Chang, Chan Il; Lee, Dong-Ki; Choi, Hee Cheul

doi:10.1007/s12274-008-8038-z

Cited by 27 publications

(17 citation statements)

References 26 publications

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“…Liu et al showed previously that DNA could be immobilized on pyrene derivatives of functionalized CNTs[ 44 ]. Other than these pyrene derivatives, single stranded DNA can also be used to immobilize CNTs[ 45 ], however DNA can be cleaved by serum, suggesting non-covalent reactions may not be stable in some cases[ 46 ]. For covalent binding, reactive groups are usually formed by various oxidation methods[ 37 , 47 ] which allow for further modifications to enhance polymer[ 38 ], protein[ 48 ] and DNA[ 33 ] attachment.…”

Section: Resultsmentioning

confidence: 99%

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

et al. 2013

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Cancer has arisen to be of the most prominent health care issues across the world in recent years. Doctors have used physiological intervention as well as chemical and radioactive therapeutics to treat cancer thus far. As an alternative to current methods, gene delivery systems with high efficiency, specificity, and safety that can reduce side effects such as necrosis of tissue are under development. Although viral vectors are highly efficient, concerns have arisen from the fact that viral vectors are sourced from lethal diseases. With this in mind, rod shaped nano-materials such as carbon nanotubes (CNTs) have become an attractive option for drug delivery due to the enhanced permeability and retention effect in tumors as well as the ability to penetrate the cell membrane. Here, we successfully engineered poly (lactic-co-glycolic) (PLGA) functionalized CNTs to reduce toxicity concerns, provide attachment sites for pro-apoptotic protein caspase-3 (CP3), and tune the temporal release profile of CP3 within bone cancer cells. Our results showed that CP3 was able to attach to functionalized CNTs, forming CNT-PLGA-CP3 conjugates. We show this conjugate can efficiently transduce cells at dosages as low as 0.05 μg/ml and suppress cell proliferation up to a week with no further treatments. These results are essential to showing the capabilities of PLGA functionalized CNTs as a non-viral vector gene delivery technique to tune cell fate.

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Section: Resultsmentioning

confidence: 99%

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

et al. 2013

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“…39 However, there are reports suggesting that noncovalent binding of biological molecules may not remain stable in serum containing situations. 40 Thus, based on our goal to provide cell attachment sites on CNTs, we functionalized CNTs through covalent reactions.…”

Section: Resultsmentioning

confidence: 99%

Alignment of Carbon Nanotubes: An Approach to Modulate Cell Orientation and Asymmetry

et al. 2014

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Stem cells offer a promising tool in tissue engineering strategies, as their differentiated derivatives can be used to reconstruct most biological tissues. These approaches rely on controlling the biophysical cues that tune the ultimate fate of cells. In this context, significant effort has gone to parse out the role of conflicting matrix-elicited signals (e.g., topography and elasticity) in regulation of macroscopic characteristics of cells (e.g., shape and polarity). A critical hurdle, however, lies in our inability to recapitulate the nanoscale spatiotemporal pattern of these signals. The study presented in this manuscript took an initial step to overcome this challenge by developing a carbon nanotube (CNT)-based substrate for nanoresolution control of focal adhesion formation and cell alignment. The utility of this system was studied using human umbilical vascular endothelial cells (HUVECs) and human embryonic stem cells (hESCs) at a single cell level. Our results demonstrated the ability to control cell orientation by merely controlling the alignment of focal adhesions at a nanoscale size. Our long-term vision is to use these nanoengineered substrates to mimic cell orientation in earlier development and explore the role of polarity in asymmetric division and lineage specification of dividing cells.

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“…Multiple studies have utilized this pi-pi interaction to noncovalently bind the CNT surface to porphyrin (71) or pyrene (72)(73)(74), which later allowed for further functionalization of proteins on the CNT surface. Other noncovalent bonding included pi-pi stacking between the CNT surface and DNA (75), although there have been concerns regarding the serum stability of DNA-wrapped CNTs owing to potential enzyme activity that may degrade the DNA wrapping (76). Amphiphilic polysaccharides and their derivatives, such as starch (77) and dextran sulfate (78), have also been employed to enfold SWNTs, enhancing their biocompatibility and solubility in biological solutions and mitigating their potential toxicity.…”

Section: Functionalization Of Cnts and Its Hybridsmentioning

confidence: 99%

Advanced contrast nanoagents for photoacoustic molecular imaging, cytometry, blood test and photothermal theranostics

Zerda

Kim

Galanzha

et al. 2011

Contrast Media & Molecular

108

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Various nanoparticles have raised significant interest over the past decades for their unique physical and optical properties and biological utilities. Here we summarize the vast applications of advanced nanoparticles with a focus on carbon nanotube (CNT)-based or CNT-catalyzed contrast agents for photoacoustic (PA) imaging, cytometry and theranostics applications based on the photothermal (PT) effect. We briefly review the safety and potential toxicity of the PA/PT contrast nanoagents, while showing how the physical properties as well as multiple biological coatings change their toxicity profiles and contrasts. We provide general guidelines needed for the validation of a new molecular imaging agent in living subjects, and exemplify these guidelines with single-walled CNTs targeted to αvβ3, an integrin associated with tumor angiogenesis, and golden carbon nanotubes targeted to LYVE-1, endothelial lymphatic receptors. An extensive review of the potential applications of advanced contrast agents is provided, including imaging of static targets such as tumor angiogenesis receptors, in vivo cytometry of dynamic targets such as circulating tumor cells and nanoparticles in blood, lymph, bones and plants, methods to enhance the PA and PT effects with transient and stationary bubble conjugates, PT/PA Raman imaging and multispectral histology. Finally, theranostic applications are reviewed, including the nanophotothermolysis of individual tumor cells and bacteria with clustered nanoparticles, nanothrombolysis of blood clots, detection and purging metastasis in sentinel lymph nodes, spectral hole burning and multiplex therapy with ultrasharp rainbow nanoparticles.

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Effect of nucleases on the cellular internalization of fluorescent labeled DNA-functionalized single-walled carbon nanotubes

Cited by 27 publications

References 26 publications

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

PLGA-Carbon Nanotube Conjugates for Intercellular Delivery of Caspase-3 into Osteosarcoma Cells

Alignment of Carbon Nanotubes: An Approach to Modulate Cell Orientation and Asymmetry

Advanced contrast nanoagents for photoacoustic molecular imaging, cytometry, blood test and photothermal theranostics

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