2022
DOI: 10.7324/jabb.2023.110227
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Effect of nutrient media enhanced with plant growth regulators on genetic stability in sub-cultures of Digitalis purpurea callus

Abstract: This study investigates the effects of nutrient media on the genetic stability of cell lines of callus produced by Digitalis purpurea leaves explants. Four cell lines of D. purpurea callus were selected after inducing 80 sub-cultures of callus in four different nutrient media: Vollosovich (5C01) medium, Murashige and Skoog (MS), Linsmaier and Skoog (LS), and Gamborg (B5) were compared with control samples of seeds and in vitro grown plant (micropropagation). DNA was isolated from the callus by cetyltrimethylam… Show more

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“…According to studies, tissue culture conditions with an imbalanced hormone ratio can increase polyploid plants. In contrast, their absence or insufficient use might lead to the production of typical plants [29,30]. In addition to the hormone concentration, the type of hormone utilized in culture media can also result in somaclonal variation [31].…”
Section: Discussionmentioning
confidence: 99%
“…According to studies, tissue culture conditions with an imbalanced hormone ratio can increase polyploid plants. In contrast, their absence or insufficient use might lead to the production of typical plants [29,30]. In addition to the hormone concentration, the type of hormone utilized in culture media can also result in somaclonal variation [31].…”
Section: Discussionmentioning
confidence: 99%