Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)

Jerez, Celia G.; Malapascua, José Romel; Sergejevová, Magda; Figueroa, Félix L.; Masojídek,

doi:10.1007/s10126-015-9664-6

Cited by 45 publications

(21 citation statements)

References 73 publications

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“…Other strategies to increase the starch content have been considered. Not only macronutrients, such as phosphorus and sulphur starvation might be used [82,83], but also micronutrients starvation as iron, manganese or zinc [84], or inhibition of nuclear DNA replication and cytoplasmic proteo-synthesis [81,85]. These findings are in line with our data: culture conditions that affect growth increase the starch accumulation in microalgal biomass.…”

Section: Discussionsupporting

confidence: 90%

How to combine CO2 abatement and starch production in Chlorella vulgaris

et al. 2018

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Microalgae production has gained attention in recent years as promising systems for CO 2 abatement as well as a source of proteins, pigments, vitamins, lipids, and carbohydrates. Particularly, starch can be used for bioethanol production in a well-established fermentative process. The aim of this work was to maximize and model biomass productivity and CO 2 assimilation in continuous cultures of Chlorella vulgaris. The following culture parameters were studied: dilution rate, pH, temperature, light intensity, and nitrogen supply. The proposed model (r 2 = 0.95) predicted a maximum biomass productivity of 0.7 g L −1 d −1 and CO 2 assimilation of 1.3 g L −1 d −1. The experimental data agreed with these predictions, resulting in a maximum biomass productivity of 0.67 g L −1 d −1 (resulting in a CO 2 assimilation of 1.23 g L −1 d −1). In addition, the starch content was determined, and the results were used as input into a second model, which aimed at predicting starch accumulation during CO 2 abatement processes (r 2 = 0.84). This second model predicted a daily and continuous production of biomass with a maximum starch content of 0.25 g g −1 d −1 (25% dcw), but under different culture conditions than those found for maximizing biomass productivity and CO 2 assimilation. The maximum starch content experimentally determined was 0.2 g g −1 d −1 (20% dcw). Thus, to implement a biological system for CO 2 abatement coupled to starch accumulation, it is necessary to find a compromise between these two processes. Hence, although yield in both processes would be reduced, a simultaneous process for CO 2 mitigation and starch production would be feasible.

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Section: Discussionsupporting

confidence: 90%

How to combine CO2 abatement and starch production in Chlorella vulgaris

et al. 2018

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“…1c ). The increased level in cell growth was similar to that observed for C. kessleri (data not shown) and other algal species 22 under N-starved conditions. The simultaneous facilitation of TG accumulation would be explained by limited inclusion of N-, S-, and Fe-sources, at least in the medium 8 10 .…”

Section: Discussionsupporting

confidence: 86%

Hyperosmosis and its combination with nutrient-limitation are novel environmental stressors for induction of triacylglycerol accumulation in cells of Chlorella kessleri

Hirai

Hayashi

Hasegawa

et al. 2016

Sci Rep

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Triacylglycerols of oleaginous algae are promising for production of food oils and biodiesel fuel. Air-drying of cells induces triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, therefore, it seems that dehydration, i.e., intracellular hyperosmosis, and/or nutrient-limitation are key stressors. We explored this possibility in liquid-culturing C. kessleri cells. Strong hyperosmosis with 0.9 M sorbitol or 0.45 M NaCl for two days caused cells to increase the triacylglycerol content in total lipids from 1.5 to 48.5 and 75.3 mol%, respectively, on a fatty acid basis, whereas nutrient-limitation caused its accumulation to 41.4 mol%. Even weak hyperosmosis with 0.3 M sorbitol or 0.15 M NaCl, when nutrient-limitation was simultaneously imposed, induced triacylglycerol accumulation to 61.9 and 65.7 mol%, respectively. Furthermore, culturing in three-fold diluted seawater, the chemical composition of which resembled that of the medium for the combinatory stress, enabled the cells to accumulate triacylglycerol up to 24.7 weight% of dry cells in only three days. Consequently, it was found that hyperosmosis is a novel stressor for triacylglycerol accumulation, and that weak hyperosmosis, together with nutrient-limitation, exerts a strong stimulating effect on triacylglycerol accumulation. A similar combinatory stress would contribute to the triacylglycerol accumulation in air-dried C. kessleri cells.

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“…In previous experiment (data not shown), the growth rate was very low if the initial cell density was lower than 0.1 g L −1 at the second stage. For the cultivation of Chlorella fusca under 1000 μmol m −2 s −1 , the initial cell density was 1.0 g L −1 [25]. Higher initial cell density could tolerate the environmental stress but it was not a good choice for improving carbohydrate productivity of Chlorella sp.…”

Section: Resultsmentioning

confidence: 99%

Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution

Cheng

Yuan

et al. 2017

Biotechnol Biofuels

127

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BackgroundMicroalgae are highly efficient cellular factories that capture CO2 and are also alternative feedstock for biofuel production. Carbohydrates, proteins, and lipids are major biochemical components in microalgae. Carbohydrates or starch in microalgae are possible substrates in yeast fermentation for biofuel production. The carbon partitioning in microalgae could be regulated through environmental stresses, such as high concentration of CO2, high light intensity, and nitrogen starvation conditions. It is essential to obtain carbohydrate-rich microalgae via an optimal bioprocess strategy.ResultsThe carbohydrate accumulation in a CO2 tolerance strain, Chlorella sp. AE10, was investigated with a two-stage process. The CO2 concentration, light intensity, and initial nitrogen concentration were changed drastically in both stages. During the first stage, it was cultivated over 3 days under 1% CO2, a photon flux of 100 μmol m−2 s−1, and 1.5 g L−1 NaNO3. It was cultivated under 10% CO2, 1000 μmol m−2 s−1, and 0.375 g L−1 NaNO3 during the second stage. In addition, two operation modes were compared. At the beginning of the second stage of mode 2, cells were diluted to 0.1 g L−1 and there was no cell dilution in mode 1. The total carbohydrate productivity of mode 2 was increased about 42% compared with that of mode 1. The highest total carbohydrate content and the highest starch content of mode 2 were 77.6% (DW) and 60.3% (DW) at day 5, respectively. The starch productivity was 0.311 g L−1 day−1 and the total carbohydrate productivity was 0.421 g L−1 day−1 in 6 days.ConclusionsIn this study, a novel two-stage process was proposed for improving carbohydrate and starch accumulation in Chlorella sp. AE10. Despite cell dilution at the beginning of the second stage, environmental stress conditions of high concentration of CO2, high light intensity, and limited nitrogen concentration at the second stage were critical for carbohydrate and starch accumulation. Although the cells were diluted, the growths were not inhibited and the carbohydrate productivity was improved. These results were helpful to establish an integrated approach from CO2 capture to biofuel production by microalgae.

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Effect of Nutrient Starvation under High Irradiance on Lipid and Starch Accumulation in Chlorella fusca (Chlorophyta)

Cited by 45 publications

References 73 publications

How to combine CO2 abatement and starch production in Chlorella vulgaris

How to combine CO2 abatement and starch production in Chlorella vulgaris

Hyperosmosis and its combination with nutrient-limitation are novel environmental stressors for induction of triacylglycerol accumulation in cells of Chlorella kessleri

Improving carbohydrate and starch accumulation in Chlorella sp. AE10 by a novel two-stage process with cell dilution

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