Effect of Nutrients on Cellulolytic and Pectolytic Enzyme Production of Alternaria Alternata a Leaf Spot Pathogen of Teak (Tectona Grandis L.F.)

Abstract

Alternaria alternata is a potential pathogen of Tectona grandis L.f., was isolated from diseased Tectona grandis L.f. leaves from Nashik and used for the present study. Pathogen was grown on the Czapek-Dox liquid medium substituting or adding different carbon, nitrogen to study cellulolytic and pectolytic enzyme production and total phenol production. The activity of enzyme was observed on the 8th day of incubation period. A great extent of growth variation was observed on different carbon, nitrogen. Among the carbon source) the maximum loss in percentage viscosity maximum in lactose and fructose. While minimum in glucose and dextrose as compared to other nitrogen source. From nitrogen source the cellulolytic enzyme activity was maximum in control and cobalt nitrate followed by similar activity in potassium nitrate and nickel nitrate. While minimum cellulolytic enzyme activity was seen in barium nitrate.. Variation was also observe in pectolytic enzyme activity. the cellulolytic enzyme activity was maximum in glucose while minimum in dextrose as compared to lactose, control and fructose.. From nitrogen source the cellulose activity was maximum in potasium nitrate and minimum in cobalt nitrate as compared to nickel nitrate, barium nitrate and control.