Effect of nutrition and Enteromyxum leei infection on gilthead sea bream Sparus aurata intestinal carbohydrate distribution

Mucins as Diagnostic and Prognostic Biomarkers in a Fish-Parasite Model: Transcriptional and Functional Analysis

“…In trial 2, this down-regulation at the PI was confirmed in RCPT fish, regardless of the diet, but no effect of the diet was found in CTRL fish. This is in accordance with previous results using cytochemistry, in which the strongest reduction of GC positive for different types of mucins was observed at the PI of E. leei -infected fish [19].…”

“…MUC13 is expressed abundantly by colorectal [36], ovarian [37] and gastric [38] human cancers, and is considered an early marker for cancer screening [39]. The down-regulation of Muc13 in infected GSB, particularly at the PI, is in agreement with the significant reduction of GC positive for sialic acid in early infected fish and the fact that it was the most reduced type of GC in fish with a high intensity of infection [19], since Muc13 is the predominant sialomucin. Furthermore, this lack of regulation could contribute to the negative inflammatory effects of the enteromyxosis, since a protective role for Muc13 in the colonic murine epithelium has been shown [40].…”

“…Previous histochemical analyses did not reveal statistically significant differences between the three intestinal segments in the same CTRL animals for any of the studied mucins (neutral, acidic, sialomucins). However, the VO diet induced a significant decrease of GC with neutral and acidic mucins in the AI and MI, and also of those with carboxylic mucins and sialic acid in the MI in CTRL fish [19], but not in PI. Therefore, with the study of the expression levels, we went further in the mucin analysis and were able to detect a mucin (intestinal mucin) that is clearly down-regulated both by the diet and by the infection at PI.…”

“…In a previous study of the mucosal carbohydrate pattern of the intestine of GSB, the VO diet produced a significant decrease of goblet cells (mucins secreting cells) with neutral and acidic mucins in the anterior intestine and middle intestine, and also of those with carboxylic mucins and sialic acid in the middle intestine. In addition, E. leei infection had a strong depletion effect on the number of goblet cells, which was stronger in VO-fed fish [19]. Thus, our experimental hypothesis is to assess if this different health phenotype is explained, at least in part, by different nutritionally-mediated effects on the intestine-mucin gene expression pattern and regulation.…”

Reproduction, olfaction and dominance behaviour in Senegalese sole (Solea senegalensis)

“…Metals such as copper or cadmium increase the number of goblet cells and mucus secretion imposing severe behavioural and physiological alterations due to smelling interference (Julliard et al, 1993;Tierney et al, 2010;Williams and Gallagher, 2013). Also, starving conditions and diets based on vegetable oils decrease the number of intestinal goblet cells and expression of mucus-related genes that in turn increase susceptibility to disease outbreaks (Estensoro et al, 2012;Li et al, 2014;Perez-Sanchez et al, 2013). Moreover, genetic families susceptible to disease can also display a higher expression of genes related with mucus production in gill and skin mucosa (Peatman et al, 2013).…”