Effect of Obese and Lean Zucker Rat Sera on Human and Rat Prostate Cancer Cells: Implications in Obesity-Related Prostate Tumor Biology

Lamarre, Neil S.; Ruggieri, Michael R.; Braverman, Alan S.; Gerstein, Matthew; Mydlo, Jack H.

doi:10.1016/j.urology.2006.09.063

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…The excess of fatty acids occurs in the blood of obese individuals, with 30‐40% of them corresponding to palmitate in a concentration close to 100 μM . Additionally, studies have shown that treatment of tumor cells with the serum of obese subjects enhances cell proliferation . In this sense, our data corroborate the literature showing that high concentrations of the saturated fatty acid palmitate can overstimulate cell proliferation in both PNT1A and PC3 cells.…”

Section: Discussionsupporting

confidence: 87%

Stimulating effect of palmitate and insulin on cell migration and proliferation in PNT1A and PC3 prostate cells: Counteracting role of metformin

et al. 2018

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Section: Discussionsupporting

confidence: 87%

Stimulating effect of palmitate and insulin on cell migration and proliferation in PNT1A and PC3 prostate cells: Counteracting role of metformin

et al. 2018

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“…31 As obesity is associated with larger tumors we sought to determine if sera from obese mice increases proliferation in prostate cancer cells. Similar to the study by Lamarre et al, 32 obesity-related factors in sera increased proliferation in prostate cancer cells compared with control. LNCaP and PacMetUT1 exposed to obese sera shows an increase in proliferation but not Obese FBS Figure 3 Sera from obese mice increases invasive capacity.…”

Section: Discussionsupporting

confidence: 85%

Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells

Price

Cavazos

Angel

et al. 2012

Prostate Cancer Prostatic Dis

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BACKGROUND: Obesity is associated with larger tumors, shorter time to PSA failure, and higher Gleason scores. However, the mechanism(s) by which obesity promotes aggressive prostate cancer remains unknown. We hypothesize that circulating factors related to obesity promote prostate cancer progression by modulating components of the metastatic cascade.METHODS: Male C57BL/6 mice (6 weeks) were fed an ad libitum diet-induced obesity (60% fat) or control diet (10% fat) for 12 weeks. Serum was collected, metabolic and inflammatory proteins were measured by an antibody array. Sera were used to measure, in vitro, characteristics of a metastatic phenotype. RESULTS:Comparable to obese men, obese sera contained higher levels or leptin, vascular endothelial growth factor, PAI-1, interleukin-6 (IL-6) and lower levels of testosterone. In prostate cells, serum was used to assess: proliferation, invasion, migration, epithelial-mesenchymal-transition (EMT) and matrix metalloproteinase (MMP) activity. LNCaP and PacMetUT1 cells exposed to obese sera increased proliferation, whereas PrEC and DU145 were unaffected. LNCaP, PacMetUT1 and DU145 cancer cells exposed to obese sera resulted in increased invasion, migration and MMP-9 activity. Prostate cancer cells exposed to obese sera showed increased vimentin, dispersion of e-cadherin and b-catenin from the plasma membrane. CONCLUSION:We report, prostate cancer cells exposed to sera from obese mice increases proliferation, invasion, migration, MMP activity and induces changes in proteins critical for EMT.

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“…Price and colleagues (2012) showed that serum from obese mice containing high concentrations of IL-6, vascular endothelial growth factor, and leptin was able to increase prostate cancer cell proliferation, invasion, migration, and matrix metalloproteinase activity. Similar results were also reported by Lamarre et al (2007), who found that serum from obese rats significantly increased the mitogenic response of prostate cancer cells in vitro.…”

Section: Discussionsupporting

confidence: 90%

Serum from morbidly obese patients affects melanoma cell behavior in vitro

Mocellin

Bratti

Silva

et al. 2022

Braz. J. Pharm. Sci.

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Here we examined whether serum from obese patients could create a growth-enhancing microenvironment that alters gene expression in BRAF-and NRAS-mutated melanoma cell lines. SK-Mel-28 (BRAF-mutated) and Sk-Mel-147 (NRAS-mutated) cells were treated with pooled serum from 10 severely obese patients (BMI > 40 kg/m 2 ), pooled serum from 6 healthy lean individuals (BMI = 18.5-24.9 kg/m 2 ), or recombinant TNF-α. We found that obese patient serum enhanced migration capacity and increased NRAS expression levels in both BRAF-and NRAS-mutated melanoma cells. Although TNF-α is the major obesity-related cytokine and TNF-α levels were found to be increased in the serum of obese individuals, this cytokine made only a modest contribution to the migration capacity of melanoma cells. These results indicate that other components present in the serum of severely obese patients may be responsible for enhancing the migration capacity of melanoma cells. As TNF-α alone did not seem to significantly affect tumor cell behavior, anti-tumor strategies aimed at blocking TNF-α should be considered with caution in future studies, particularly when in vitro models are used as screening platforms for antitumor activity.

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Effect of Obese and Lean Zucker Rat Sera on Human and Rat Prostate Cancer Cells: Implications in Obesity-Related Prostate Tumor Biology

Cited by 8 publications

References 24 publications

Stimulating effect of palmitate and insulin on cell migration and proliferation in PNT1A and PC3 prostate cells: Counteracting role of metformin

Stimulating effect of palmitate and insulin on cell migration and proliferation in PNT1A and PC3 prostate cells: Counteracting role of metformin

Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells

Serum from morbidly obese patients affects melanoma cell behavior in vitro

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