Effect of obesity and insulin sensitivity on adiponectin isoform distribution

Kaser, Susanne; Tatarczyk, Tobias; Stadlmayr, Andreas; Ciardi, Christian; Ress, Claudia; Tschoner, Alexander; Sandhofer, A.; Paulweber, Bernhard; Ebenbichler, Christoph; Patsch, Josef R.

doi:10.1111/j.1365-2362.2008.02028.x

Cited by 80 publications

(65 citation statements)

References 46 publications

(62 reference statements)

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“…Several evidences indicate that both total ACRP30 and HMW oligomer levels decrease in obesity (31,32,33) and increase after weight loss (34). Interestingly, the present study also reveals an involvement of MMW, LMW, and monomeric forms of ACRP30 in the improvement of obesity state.…”

Section: Discussionsupporting

confidence: 72%

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Rosa

Monaco

Capasso

et al. 2013

European Journal of Endocrinology

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Objective: Adiponectin is an adipocytokine that exerts beneficial effects on obesity and related disorders by two receptors (ADIPORs). Adiponectin is produced as a monomer that circulates in serum as different oligomers. The oligomerization state and the tissue expression of adiponectin and ADIPORs are linked to its biological activities. In this study, the levels of total adiponectin and its oligomers were evaluated in relation to obesity and surgical weight loss. The expression of adiponectin and ADIPORs was analyzed in visceral and subcutaneous adipose tissues of obese patients. Design and methods: In 25 obese patients and 44 age-and sex-matched controls, the serum levels of adiponectin and its oligomers were measured and compared by ELISA, western blotting, and gel filtration. The expression of adiponectin and ADIPORs in both adipose tissues was evaluated by realtime quantitative PCR and western blotting. Results: The amount of each adiponectin oligomer, including the monomer, increases after weight loss. The reduced circulating levels of adiponectin and its oligomers are not associated with the adipose tissue depot-specific expression of adiponectin and ADIPORs. Conclusions: Our results suggest that in patients, adiposity is associated with the serum concentrations of adiponectin and its oligomers but not with adipose tissue depot-specific expression of adiponectin and ADIPORs. In particular, the increase in adiponectin monomer levels could probably be related to the improvement of the whole-body energy metabolism then being involved in the improvement of adipose tissue function after weight loss. This work indicates the importance of assessing the whole adiponectin oligomeric profile as further potential indicators of adipose tissue functions in obesity.

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Section: Discussionsupporting

confidence: 72%

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Rosa

Monaco

Capasso

et al. 2013

European Journal of Endocrinology

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“…Overall, these data reflect the insulin resistant state generally associated with old age (Narimiya et al 1984;DeFronzo 1981). Interestingly, the [HMW/ total adiponectin] ratio was significantly lower in aged compared to adult mice, supporting a role for HMW adiponectin as the insulin-sensitizing form of this hormone (Kaser et al 2008). However, the ratio of [total adiponectin/leptin], another suggested indicator of insulin sensitivity ) tended to increase in aged mice compared to the adult group.…”

Section: Discussionmentioning

confidence: 51%

“…Regarding leptin and insulin, the observed correlations with fat mass were consistent with previous data (Considine et al 1996;Frederich et al 1995;Maffei et al 1995;Carantoni et al 1998). However, for circulating adiponectin, an inverse relationship with body fat mass is usually observed (Kaser et al 2008). Also, although mainly related to advanced disease states, adiponectin levels increase in cachexia and may predict mortality (Diez and Iglesias 2010).…”

Section: Discussionmentioning

confidence: 99%

Decreased insulin sensitivity and increased oxidative damage in wasting adipose tissue depots of wild-type mice

Sackmann-Sala

Berryman

Lubbers

et al. 2011

AGE

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Unintentional weight loss (wasting) in the elderly is a major health concern as it leads to increased mortality. Several studies have focused on muscle loss, but little is known about the mechanisms giving rise to loss of fat mass at old ages. To investigate potential mechanisms, white adipose tissue (WAT) characteristics and proteomic profiles were compared between adult (10-12-month-old) and aged (22-24-month-old) wild-type mice. Four individual WAT depots were analyzed to account for possible depot-specific differences. Proteomic profiles of WAT depots, along with body weights and compositions, plasma levels of insulin, leptin and adiponectin, insulin tolerance, adipocyte sizes, and products of oxidative damage in each WAT depot were determined. We found that lean mass remained constant while fat mass and insulin tolerance were decreased in old age, as were adipocyte sizes in the WAT depots. Proteomic results showed increased levels of enolase, pyruvate dehydrogenase E1β, NAD + −dependent isocitrate dehydrogenase α, and ATP synthase subunit β, and decreased levels of carbonic anhydrase 3 in WAT of aged mice. These data suggest increased aerobic glucose oxidation in wasting WAT, consistent with AGE (2012) decreased insulin signaling. Also, Cu/Zn superoxide dismutase and two chaperones were increased in aged WAT depots, indicating higher stress resistance. In agreement, lipid peroxidation (HNE-His adducts) increased in old age, although protein oxidation (carbonyl groups) showed no increase. In conclusion, features of wasting WAT were similar in the four depots, including decreased adipocyte sizes and alterations in protein expression profiles that indicated decreased insulin sensitivity and increased lipid peroxidation.

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“…17,18 Plasma levels of the HMW isoform and the ratio of HMW to total adiponectin are more closely associated with glucose tolerance and insulin sensitivity than total adiponectin. 19,20 Taking into account the selective decreased levels of circulating HMW adiponectin in individuals with obesity 21,22 and the different endocrine activity of SAT and VAT, 23 the fat depot-specific alterations of adiponectin isoforms production may be expected.…”

Section: Introductionmentioning

confidence: 99%

The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue

et al. 2011

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OBJECTIVE: Hypoadiponectinemia observed in obesity is associated with insulin resistance, diabetes and atherosclerosis. The aim of the present study was to investigate secretion of adiponectin and its multimeric isoforms by explants derived from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in obese and non-obese subjects. DESIGN: Paired samples of SAT and VAT and blood samples were obtained from 23 subjects (10 non-obese and 13 obese) undergoing elective abdominal surgery. Total adiponectin quantities and adiponectin isoforms were measured in conditioned media of explants derived from SAT and VAT using enzyme-linked immunosorbent assay and non-denaturing western blot, respectively. RESULTS: Total adiponectin plasma levels were lower in obese than in non-obese subjects (Po0.05). Secretion of total adiponectin in adipose tissue (AT) explants was lower in obese than in non-obese subjects in SAT (Po0.05) but not in VAT. In both, SAT and VAT, the most abundant isoform released into conditioned media was the high-molecular weight (HMW) form. Its relative proportion in relation to total adiponectin was higher in conditioned media of explants from both fat depots when compared with plasma (Po0.001). The proportion of secreted HMW vs total adiponectin was higher in VAT than in SAT explants in the group of non-obese individuals (49.3 ± 3.1% in VAT vs 40.6 ± 2.8% in SAT; Po0.01), whereas no difference between the two depots was found in obese subjects (46.2 ± 3.0 % in VAT vs 46.0 ± 2.4 % in SAT). CONCLUSION: Obesity is associated with the decrease of total adiponectin secretion in SAT. The profile of adiponectin isoforms secreted by SAT and VAT explants differs from that in plasma. Secretion of total adiponectin and HMW isoform of adiponectin are different in obese and non-obese subjects in relation to AT depot.

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Effect of obesity and insulin sensitivity on adiponectin isoform distribution

Cited by 80 publications

References 46 publications

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Adiponectin oligomers as potential indicators of adipose tissue improvement in obese subjects

Decreased insulin sensitivity and increased oxidative damage in wasting adipose tissue depots of wild-type mice

The impact of obesity on secretion of adiponectin multimeric isoforms differs in visceral and subcutaneous adipose tissue

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