Effect of obesity on HDL and LDL particle sizes in carriers of the null P207L or defective D9N mutation in the lipoprotein lipase gene: the Québec LipD Study

Abstract

BACKGROUND:We have recently demonstrated that French Canadians bearing a mutation in the lipoprotein lipase (LPL) gene present an impaired lipoprotein-lipid profile characterized by small low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles compared with healthy subjects. It has also been documented that obesity has a significant impact on HDL and LDL particle sizes. OBJECTIVE: To examine the extent to which obesity modulates HDL and LDL particle sizes among carriers of mutations in the LPL gene. SUBJECTS: Analyses were carried out in 206 heterozygous carriers of the D9N mutation (N ¼ 118) or the P207L mutation (N ¼ 88). MEASUREMENTS: Lipoprotein particle sizes were measured on whole plasma by nondenaturing polyacrylamide gradient gel electrophoresis. RESULTS: In general, body mass index (BMI) and waist circumference were significant correlates of LDL and HDL particle sizes among heterozygous carriers of the P207L or D9N mutation in the LPL gene, with relatively similar associations among men and women. Multivariate analyses indicated that variations in waist circumference but not BMI were an independent predictor of variations in both HDL particle size (5.2%, P ¼ 0.0005) and LDL particle size (5.9%, P ¼ 0.01) in the entire group of heterozygotes for LPL mutation in a model that included the nature of the LPL mutation (D9N vs P207L), gender, age, cholesterol and plasma TG levels. Interestingly, there was a significant interaction between plasma TG levels and waist circumference or BMI in modulating HDL particle size. Indeed, an increased waist circumference or BMI was associated with a significant reduction in HDL particle size among subjects with plasma TG levels r3.5 mmol/l, but not among those with marked hypertriglyceridemia (TG levels 43.5 mmol/l). CONCLUSION: These results suggest that abdominal obesity, more so that overall obesity, is an important determinant of variations in LDL and HDL particle size among heterozygous carriers of mutations in the LPL gene, perhaps further contributing to modulate the risk of CHD in these individuals.

Body Mass Index and Associated Factors of Obesity and Underweight in a General Out-patient Population in a Tertiary Hospital in Nigeria

“…It was also observed that abnormal serum HDL occurred more in underweight subjects, contrary to the report documented in another study that showed an association between abnormal HDL and obesity. [30] Serum HDL predicted obesity in this study. Perhaps, our study subjects might have other morbidities that could account for this contrast, as they were patients presenting in the out-patient clinic.…”

“…This is in agreement with reports that have shown an association between obesity and LDL hypercholesterolemia. [29,30] Serum LDL is the major determinant of serum total cholesterol, and therefore increases as serum total cholesterol increases. [30] The association between BMI and serum HDL was significant, (df=3, p<0.001), as found in this study.…”

“…[29,30] Serum LDL is the major determinant of serum total cholesterol, and therefore increases as serum total cholesterol increases. [30] The association between BMI and serum HDL was significant, (df=3, p<0.001), as found in this study. It was also observed that abnormal serum HDL occurred more in underweight subjects, contrary to the report documented in another study that showed an association between abnormal HDL and obesity.…”

The Effect of Orlistat, Green Coffee Bean Extract, and Its Combinations on Lipid Profi le and Adiponectin Levels

“…Lipoprotein lipase merupakan enzim yang berperan penting dalam hidrolisis lemak. Kelainan pada enzim ini akan mengakibatkan peningkatan TG, LDL, VLDL, dan penurunan HDL (Ruel et al, 2003).…”

Visceral adipose tissue accumulation, secretory phospholipase A2-IIA and atherogenecity of LDL

“…4,5 The phospholipase (PLA 2 ) family comprises a group of enzymes that hydrolyze the sn-2 ester bond in the glyceroacyl phospholipids present in lipoproteins and cell membranes. 6 Group IIA (-IIA) sPLA 2 is an acute-phase protein for which plasma levels were reported to correlate with that of C-reactive protein (CRP) and to be an independent risk factor and predictor of coronary heart disease.…”