Effect of obesity on inflammatory markers and renal functions

Cındık, Nimet; Baskın, Esra; Ağras, Pınar Işık; Kinik, Sibel Tulgar; Turan, M; Saatçi, U

doi:10.1080/08035250500277101

Cited by 40 publications

(24 citation statements)

References 24 publications

(20 reference statements)

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“…Though the composition and concentration of urine vary with physiologic state, there was less than 10±10% (mean±standard deviation) difference in total protein abundance among individual specimens, as ascertained by using gel image densitometry (Fig. 1), similar to earlier studies of urine of children [28–30]. …”

Section: Resultssupporting

confidence: 83%

Urine proteomics for profiling of human disease using high accuracy mass spectrometry

Kentsis

Monigatti

Dorff

et al. 2009

Proteomics Clinical Apps

120

109

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Knowledge of the biologically relevant components of human tissues has enabled the invention of numerous clinically useful diagnostic tests, as well as non-invasive ways of monitoring disease and its response to treatment. Recent use of advanced MS-based proteomics revealed that the composition of human urine is more complex than anticipated. Here, we extend the current characterization of the human urinary proteome by extensively fractionating urine using ultracentrifugation, gel electrophoresis, ion exchange and reverse-phase chromatography, effectively reducing mixture complexity while minimizing loss of material. By using high-accuracy mass measurements of the linear ion trap-Orbitrap mass spectrometer and LC-MS/MS of peptides generated from such extensively fractionated specimens, we identified 2362 proteins in routinely collected individual urine specimens, including more than 1000 proteins not described in previous studies. Many of these are biomedically significant molecules, including glomerularly filtered cytokines and shed cell surface molecules, as well as renally and urogenitally produced transporters and structural proteins. Annotation of the identified proteome reveals distinct patterns of enrichment, consistent with previously described specific physiologic mechanisms, including 336 proteins that appear to be expressed by a variety of distal organs and glomerularly filtered from serum. Comparison of the proteomes identified from 12 individual specimens revealed a subset of generally invariant proteins, as well as individually variable ones, suggesting that our approach may be used to study individual differences in age, physiologic state and clinical condition. Consistent with this, annotation of the identified proteome by using machine learning and text mining exposed possible associations with 27 common and more than 500 rare human diseases, establishing a widely useful resource for the study of human pathophysiology and biomarker discovery.

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Section: Resultssupporting

confidence: 83%

Urine proteomics for profiling of human disease using high accuracy mass spectrometry

Kentsis

Monigatti

Dorff

et al. 2009

Proteomics Clinical Apps

120

109

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“…An increased GFR was observed in overweight compared with lean subjects, being significantly positively related to BMI [44] and insulin resistance [18]. …”

Section: Obesity-related Renal Injury In Childhoodmentioning

confidence: 99%

Obesity-Related Renal Injury in Childhood

Sereno

Pelliccia²,

Chiarelli³

et al. 2010

Horm Res Paediatr

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A significant increase in the prevalence of end-stage renal disease (ESRD) has been reported over the last three decades, paralleling the increasing prevalence of obesity and insulin resistance, also in the pediatric population. Overweight, obesity and the metabolic syndrome, which frequently coexist, contribute substantially to cardiovascular disease and ESRD. A higher body mass index, the presence of type 2 diabetes, hypertension and, of particular importance, reduced insulin sensitivity (IS), have recently emerged as strong independent risk factors for chronic kidney disease and ESRD. Of particular concern, the long-term cardiovascular impact of obesity, although deferred to adult life, has its origins in childhood. Clustering of cardiovascular risk factors is seen in children and adolescents with the highest degree of reduced IS, suggesting that adult consequences of obesity on target organs, including the kidney, are more likely to develop in these young people. This review will discuss the association between obesity and the risk of kidney disease, focusing on the way in which obesity and its metabolic complications may lead to renal involvement and injury, with particular regard to childhood. It is beyond the scope of this article to examine kidney disease as a component of syndromes that result in obesity in childhood.

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“…While the relation between obesity and both raised C-reactive protein (CRP) [4,5] and decreased adiponectin (including high-molecular-weight adiponectin) levels [6,7,8,9,10] in children is well documented, studies examining IL-6 levels in overweight children show conflicting findings [8,11,12,13]. Obese prepubertal Turkish children have significantly elevated IL-6 levels compared to lean controls [11], and Herder et al [12] showed a significant association between IL-6 levels and BMI (adjusted for age, sex and lipids) in a large cohort of German adolescents.…”

Section: Introductionmentioning

confidence: 99%

IL-6, IL-8 and IL-10 Levels in Healthy Weight and Overweight Children

Tam¹,

Garnett²,

Cowell³

et al. 2010

Horm Res Paediatr

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Background/Aim: In adults, studies have shown that obesity is a chronic low-grade inflammatory state characterized by altered levels of cytokines. Studies in children have mainly focused on C-reactive protein and adiponectin, and there is limited data for other inflammatory markers in healthy weight and overweight children. The aim of this study was to measure IL-6, IL-8 and IL-10 levels in healthy normal weight and overweight children at 8 and 15 years. Methods: 118 normal weight and overweight children (59 boys) from the Nepean longitudinal study were recruited at age 8 years and followed up at 15 years. Serum IL-6, IL-8 and IL-10 levels were measured at both time-points. Results: At 8 years, we found no significant differences in cytokine levels between normal weight and overweight (owt)/obese (ob) groups. However, at 15 years, owt/ob girls (n = 23) had higher levels of IL-6 (p = 0.04), IL-8 (p = 0.04) and IL-10 (p = 0.03) compared to normal weight girls (n = 36), even after adjustment for puberty; no differences were seen in boys. Conclusion:The effects of obesity on IL-6, IL-8 and IL-10 levels vary with age and sex, with owt/ob girls at 15 years showing raised IL-6, IL-8 and IL-10 levels compared to healthy weight girls.

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Effect of obesity on inflammatory markers and renal functions

Abstract: Inflammatory mediators increased significantly in obese children, and the glomerular filtration rate increased as the body mass index increased. To prevent obesity-related complications in adulthood, it is important to take measures to prevent development of obesity during childhood.

Cited by 40 publications

References 24 publications

Urine proteomics for profiling of human disease using high accuracy mass spectrometry

Urine proteomics for profiling of human disease using high accuracy mass spectrometry

Obesity-Related Renal Injury in Childhood

IL-6, IL-8 and IL-10 Levels in Healthy Weight and Overweight Children

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