Effect of ochratoxin A on aflatoxin production by <i>Aspergillus parasiticus</i>

Serafini, Mauro; Foddai, Sebastiano; Pieretti, Stefano; Tomassini, Lamberto; Nicoletti, Marcello

doi:10.1139/b91-003

Cited by 1 publication

(1 citation statement)

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“…Many health detrimental effects (carcinogenic, mutagenic and teratogenic) are associated with presence of aflatoxin B1 in agricultural products (Begum and Samajpati 2000). Aflatoxin content of agricultural produce is properly regulated in many developed countries; A aflatoxin level below 20 ng is permitted in maize for the free movement (Serafini et al 1991;Stoloff et al 1991). Aspergillus flavus is one of the major fungus of aflatoxin B1 production which contaminates agricultural commodities in field, transport and storage.…”

Section: Discussionmentioning

confidence: 99%

Characterization of aflatoxigenic Aspergillus flavus associated with aflatoxin B1 (AFB1) production in maize kernel in India

Kumari

Gogoi

Srinivasa

et al. 2020

Indian Phytopathology

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Mycotoxin-producing fungi directly impact on the yield and quality of agricultural produce. Aspergillus flavus is predominant fungi to produce aflatoxin B1 (AFB1) mycotoxin in maize. Early detection and quantification of aflatoxin B1 producing A. flavus is indispensable to mitigate maize loss. With this aim, three different detection methods (PCR, qPCR and HPLC) deployed to screen highly toxigenic and atoxigenic isolates of A. flavus. PCR analysis of four A. flavus isolates revealed possession of six toxigenic genes (aflO, aflP, aflQ, aflM, aflS and aflD), among aflO, aflP and aflQ genes are most common. In qPCR, seven isolates tested representing 5 different maize growing agroclimatic zones of India, expressed nor-1 gene. In two of the isolates, higher mean expression levels were noticed. In HPLC, aflatoxin B1 (AFB1) production capacity was tested in all isolates. About 65% of the A. flavus isolates produced aflatoxin B1 (between 43.87 and 0.88 µg/g). This study exhibited that the highly toxic isolate observed from the sample collected from Hyderabad, where maize crop is growing year round, followed by nagaon's sample from Assam, therefore these locations will need extra care during post harvest condition to minimize the risk of aflatoxin in storage.

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Section: Discussionmentioning

confidence: 99%