Effect of octadeca carbon fatty acids on microbial fermentation, methanogenesis and microbial flora in vitro

Zhang, C.M.; Guo, Yuhan; Yuan, Zhao; Wu, Y.M.; Wang, J.K.; Liu, J.X.; Zhu, Weiyun

doi:10.1016/j.anifeedsci.2008.01.005

Cited by 124 publications

(131 citation statements)

References 25 publications

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“…Mao et al (2010) found that the supplementation of 3% soybean oil depressed the population of F. succinogenes and R. flavefaciens. In addition, Zhang et al (2008) reported that supplementation of C18:3n-3, C18:2n-6, C18:1n-9 and C18:0 at 35 and 70 g/kg DM of substrate decreased the population of R. flavefaciens and F. succinogenes in vitro. In contrast, supplementation of different blends (1:1, 2.5:1.5 and 1:2.5) of sunflower oil and fish oil at 4% reduced the population of F. succinogenes but did not affect the populations of R. albus and R. flavefaciens in steers (Liu et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Rumen microbial community and nitrogen metabolism in goats fed blend of palm oil and canola oil

Adeyemi

Ahmed

Jotham

et al. 2016

Italian Journal of Animal Science

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This study assessed the influence of dietary blend of 20% palm oil and 80% canola oil on the population of rumen microbiota and nitrogen economy in goats. Twenty-four Boer bucks (4-5 months old; initial BW, 20.54 ± 0.474 kg) were randomly allotted to diets containing on a dry matter basis, 0, 4 or 8% oil blend, fed daily for 100 days and slaughtered. The rumen microbiota was examined by quantitative real-time polymerase chain reaction (PCR) using the 16S rRNA gene. The population of total protozoa and methanogens was lower (p < .05) while the population of Fibrobacter succinogenes was greater (p < .05) in goats fed oil blend compared with the control goats. The population of total bacteria was greater (p < .05) in goats fed 8% oil blend compared with those fed other diets. The population of Ruminococcus albus and Ruminococcus flavefaciens was greater (p < .05) in goats fed 4% oil blend compared with those fed other diets. Diet had no effect (p> 0.05) on microbial N yield, microbial protein synthesis, efficiency of microbial protein synthesis and the intake, retention and excretion of N. Dietary supplementation of blend of palm oil and canola oil altered rumen microbial profile but did not affect nitrogen metabolism in goats.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Rumen microbial community and nitrogen metabolism in goats fed blend of palm oil and canola oil

Adeyemi

Ahmed

Jotham

et al. 2016

Italian Journal of Animal Science

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“…Dietary polyunsaturated fatty acids (PUFA) have toxic effects on ruminal microorganisms, therefore, lipid supplementation often leads to changes in ruminal microbial populations and shifts in ruminal fermentation parameters (Zhang et al, 2008). Rumen microbes attempt to detoxify PUFA by biohydrogenation (Maia et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Vargas‐Bello‐Pérez

Cancino‐Padilla

Romero

et al. 2016

Animal

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Vegetable oils are used to increase energy density of dairy cow diets, although they can provoke changes in rumen bacteria populations and have repercussions on the biohydrogenation process. The aim of this study was to evaluate the effect of two sources of dietary lipids: soybean oil (SO, an unsaturated source) and hydrogenated palm oil (HPO, a saturated source) on bacterial populations and the fatty acid profile of ruminal digesta. Three non-lactating Holstein cows fitted with ruminal cannulae were used in a 3 × 3 Latin square design with three periods consisting of 21 days. Dietary treatments consisted of a basal diet (Control, no fat supplement) and the basal diet supplemented with SO (2.7% of dry matter (DM)) or HPO (2.7% of DM). Ruminal digesta pH, NH 3 -N and volatile fatty acids were not affected by dietary treatments. Compared with control and HPO, total bacteria measured as copies of 16S ribosomal DNA/ml by quantitative PCR was decreased ( P < 0.05) by SO. Fibrobacter succinogenes, Butyrivibrio proteoclasticus and Anaerovibrio lipolytica loads were not affected by dietary treatments. In contrast, compared with control, load of Prevotella bryantii was increased ( P < 0.05) with HPO diet. Compared with control and SO, HPO decreased ( P < 0.05) C18:2 cis n-6 in ruminal digesta. Contents of C15:0 iso, C18:11 trans-11 and C18:2 cis-9, trans-11 were increased ( P < 0.05) in ruminal digesta by SO compared with control and HPO. In conclusion, supplementation of SO or HPO do not affect ruminal fermentation parameters, whereas HPO can increase load of ruminal P. bryantii. Also, results observed in our targeted bacteria may have depended on the saturation degree of dietary oils.

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“…Fatty acids inhibit methane production through the provision of an alternate means of electron disposal, as well as by direct toxic effects on ruminal microoganisms and protozoa (McAllister et al 1996;Zhang et al 2008). With 9-25% of methane production in the rumen attributed to protozoaassociated methanogens (Newbold et al 1995), the elimination of protozoa will simultaneously reduce the associated methanogens (Jouany 1994).…”

Section: Fatty Acidsmentioning

confidence: 99%

“…The mode of action of these compounds to reduce ruminant methane emissions are quite variable, with some being directly inhibitory to methanogens (e.g. fatty acids (Zhang et al 2008), HMA (Prins et al 1972)) and/or protozoa (e.g. fatty acids (Matsumoto et al 1991)), while the same or different compounds cause a shift towards the production of propionate in the rumen (e.g.…”

Section: Introductionmentioning

confidence: 99%

Reducing in vitro rumen methanogenesis for two contrasting diets using a series of inclusion rates of different additives

et al. 2014

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Abstract. Eleven individual additives were incubated with either perennial ryegrass or with grass silage+barley grain (50 : 50) and the in vitro methane output was assessed using the gas production technique (GPT). Additives were: fatty acids (lauric, oleic, linoleic and linolenic acids), halogenated methane analogues (bromoethanesulfonate and bromochloromethane), pyromellitic diimide, statins (mevastatin and lovastatin), a probiotic (Saccharomyces cerevisiae) and an unsaturated dicarboxylic acid (fumaric acid). Each additive was included at a range of concentrations. Effects on methane output per gram of feed dry matter (DM) incubated (CH 4 /DMi) and disappeared (CH 4 /DMd), as well as other fermentation variables, were evaluated after 24 h of incubation. The addition of increased concentrations of individual fatty acids, bromoethanesulfonate and pyromellitic diimide caused a dose-dependent decline in methane output (CH 4 /DMi, CH 4 / DMd), when incubated with either perennial ryegrass or grass silage+barley grain. No methane output was detected for either feed with the addition of !5 mM bromochloromethane. The statins were ineffective inhibitors of methane output regardless of feed type. For perennial ryegrass, S. cerevisiae caused a dose-dependent decline in CH 4 /DMd and fumaric acid a dose-dependent decline in CH 4 /DMi and CH 4 /DMd. The effectiveness of lauric, oleic, linoleic and linolenic acids and bromoethanesulfonate to reduce methane output was more pronounced when incubated with grass silage+barley grain than with perennial ryegrass, and therefore the type of feed is an important component for any future in vitro and in vivo studies to be undertaken with these additives. Thus, incorporating different feed types in the initial in vitro screening protocols of all new additives is recommended.Additional keywords: in vitro total gas production technique, rumen fermentation modifiers, ruminant diets.

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Effect of octadeca carbon fatty acids on microbial fermentation, methanogenesis and microbial flora in vitro

Cited by 124 publications

References 25 publications

Rumen microbial community and nitrogen metabolism in goats fed blend of palm oil and canola oil

Rumen microbial community and nitrogen metabolism in goats fed blend of palm oil and canola oil

Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Reducing in vitro rumen methanogenesis for two contrasting diets using a series of inclusion rates of different additives

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