Effect of oligonucleotide AGAGGAGGU on protein synthesis in vitro

Trudel, Marie; Dondon, J.; Grunberg‐Manago, Marianne; Finelli, Jacqueline; Buckingham, Richard H.

doi:10.1016/s0300-9084(81)80197-6

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…A procedure of in vitro hybrid-arrested translation involving denatured DNA annealed with a population of mRNA was used to identify transcripts (12,28) and to investigate translation mechanisms (29). Oligonucleotides complementary to the Shine-Dalgarno sequence at the 3' end ofthe 16S rRNA were shown to inhibit the formation of the 70S initiation complex (30)(31)(32). The notion that anti-mRNAs could be used to control gene expression was derived from these results (33)(34)(35)(36)(37)(38)(39)(40)(41).…”

Section: Discussionmentioning

confidence: 99%

Specific inhibition of mRNA translation by complementary oligonucleotides covalently linked to intercalating agents.

Toulmé¹,

Krisch²,

Loreau³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

102

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Synthetic oligodeoxynucleotides that are covalently linked at their 3' end to an acridine derivative and are complementary to the repeated sequence UUAAAU-UAAAUUAAA adjacent to the ribosome binding site of the gene 32-encoded mRNA from phage T4 have been used to regulate the synthesis of gene 32-encoded protein in vitro. These modified, synthetic oligonucleotides specifically block the translation of gene 32-encoded mRNA with a higher efficiency than the homologous unsubstituted oligonucleotides. The inhibition produced by these short "anti-messengers" is due to the formation of specific mRNA-oligodeoxynucleotide hybrids that are stabilized by the intercalation of the acridine ring in the RNADNA duplex.

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