Effect of olive, flaxseed, and grape seed nano-emulsion essential oils on semen buffalo freezability

Khalil, Wael A.; Hassan, Mahmoud A.E.; Attia, Kandil A.A.; El-Metwaly, Hassan A.; El-Harairy, Mostafa A.; Sakr, Abdelaziz M.; Abdelnour, Sameh A.

doi:10.1016/j.theriogenology.2023.08.021

Cited by 10 publications

(1 citation statement)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it has been documented that oleic acid and palmitic acid improved sperm motility, viability, acrosomes, mitochondrial membrane potential and ATP production in chilled boar sperm [ 26 ]. According to previous studies in boar [ 33 ] and another study in buffalo [ 39 ], fatty acid antioxidants such as omegas 3, 6, 9 and DHA improved post-thawed semen quality in terms of plasma membrane integrity and acrosome integrity. It has also been reported by many studies that supplementation of omega 3 and/or 6 in feed improve not only semen qualities in boar but also reproductive performance in sows [ 38 , 40 – 44 ].…”

Section: Discussionmentioning

confidence: 97%

Camellia oil with its rich in fatty acids enhances post-thawed boar sperm quality

Khophloiklang,

Chanapiwat,

Kaeoket

2024

Acta Vet Scand

View full text Add to dashboard Cite

Background Boar sperm are highly susceptible to specific conditions during cryopreservation, leading to a significant decrease in their fertilizing potential due to damage to their membranes. Camellia oil, known for its fatty acids with antioxidant and biological properties, has not been previously explored for the cryopreservation of boar semen. This study aimed to examine the effects of camellia oil on post-thawed boar sperm quality. Boar semen ejaculates (n = 9) were collected and divided into six equal aliquots based on camellia oil concentrations (0, 0.5, 1, 1.5, 2 and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm morphology by scanning electron microscope, sperm motility using a computer-assisted sperm analyzer, sperm viability, acrosome integrity, mitochondrial function, MDA level and total antioxidant capacity. Results The results demonstrated that the supplementation of 1.5% (v/v) camellia oil showed superior post-thaw sperm qualities such as improved sperm morphology, motility, acrosome integrity and mitochondrial function by 14.3%, 14.3% and 11.7%, respectively, when compared to the control group. Camellia oil at a concentration of 1.5% (v/v) showed the lowest level of MDA (18.3 ± 2.1 µmol/L) compared to the other groups. Conclusions In conclusion, adding 1.5% (v/v) camellia oil in the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in a higher post-thawed sperm quality.

show abstract