Effect of Olive Leaves and Propolis Extracts on Growth Performance, Immunological Parameters and Economic Efficiency using Nile Tilapia (Oreochromis niloticus)

Shahin, Sara E.; Eleraky, W.; El-Gamal, Mahmoud; Hassanein, E. I.; Ibrahim, Doaa

doi:10.21608/zvjz.2019.17129.1085

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…In addition, the fish resistance to bacterial infection was evidenced in several investigations [22,[25][26][27][28]. Furthermore, PR ameliorated the harmful effects of other thermal and cytotoxic stress factors on fish physiological performance, immune response, and redox status [23,29,30].…”

Section: Introductionmentioning

confidence: 97%

“…Other advantageous qualities of PR include antioxidant capabilities [17], immune stimulation [18], anti-inflammatory activities [19], and antibacterial effects (Kroko et al, 2012). Previous research concluded the beneficial effects of PR administration on cultured fish's innate immunity, plasma biochemicals, and growth performance [20][21][22][23][24]. In addition, the fish resistance to bacterial infection was evidenced in several investigations [22,[25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Dietary propolis complementation relieves the physiological and growth deterioration induced by Flavobacterium columnare infection in juveniles of common carp (Cyprinus carpio)

Hassanien,

Alrashada,

Abbas

et al. 2023

PLoS ONE

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The current study was proposed to explore the role of dietary propolis (PR) supplementation in alleviating the negative effects of columnaris disease (CD) challenge on the growth performance, plasma biochemicals, antioxidant activity, stress indicators, and immunological reactions of common carp (Cyprinus carpio) fish. Five hundred forty common carp juveniles were evenly placed in thirty-six 100-L tanks and stocked for acclimatization to the lab conditions with a control diet within a started period of 14 days. Fish (average initial weight of 7.11±0.06 g) were randomly distributed into one of six treatment groups (6 replicate tanks × 15 fish per tank in each treatment group). Fish in the first group was assigned as a negative control without CD challenge or PR supplementation. Fish in the other five groups were challenged with CD by immersion of fish for 60 min into a 10-L water bath supplemented with 6×106 CFU/mL (median lethal dose, LD50) of pathogenic F. columnare bacteria. After infection, the fish were restored to their tanks and fed on a basal diet supplemented with PR at 0, 3, 6, 9, or 12 g/kg diet. The experimental period continued for 6 consecutive weeks in which the feed was introduced twice a day (8:00 and 15:00 h) at a rate of 2% of the fish biomass. Ten percent of water was siphoned and renewed after each meal every day, in addition to 50% of water refreshment after cleaning the tank every three days. The tanks were continuously aerated and provided with standard rearing conditions for carp fish (24.0±1.12°C, 7.7±0.22 pH, 6.3±0.16 mg/L O2, and 14L/10D photoperiod). The growth performance traits such as feed intake (FI), weight gain (WG), final weight (FW), specific growth rate (SGR), feed efficiency (FE), and cumulative mortality rates (CM) were recorded during the experimental period. At the end of the trial, blood samples were obtained from the fish to evaluate some plasma biochemicals, including aspartate aminotransaminase (AST), alanine aminotransferase (ALT), creatinine (CRE), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), antioxidant biomarkers, including total antioxidant capacity (TAOC), total superoxide dismutase (TSOD), reduced glutathione (rGSH), and catalase (CAT), stress indicators, including heterophil to lymphocyte (H/L) ratio, cortisol (COR), malondialdehyde (MDA), and myeloperoxidase (MPO), and immunological reactions, including peripheral blood leukocyte proliferation (PBLP), phagocytosis activity (PHG), lysozyme activity (LYS), alternative complement hemolytic action (ACH50), and total immunoglobulin concentration (TIG). In addition, samples of infected fish gills were taken to quantify the number of F. columnare in the PR-supplemented groups using the quantitative real-time polymerase chain reaction (qPCR) technique. The results showed that incorporating PR into the dietary ingredients of common carp has a protective effect against the challenge with F. columnare infection. There were linear and quadratic positive trends (P < 0.05) in most parameters of growth performance, plasma biochemicals, antioxidant activity, stress indicators, and immunological reactions with the increased PR-supplemented levels in the diet of infected fish. The best results were obtained when using PR at 9 g/kg in the diet, while higher levels (12 g/kg PR) showed an adverse trend in the evaluated parameters. The FI, WG, FW, SGR, and FE were improved by approximately 37, 104, 34, 73, and 49% in the fish treated with 9 g/kg PR compared to none-PR-infected fish. In addition, adding PR at the 9 g/kg diet level was the best dose that reduced the H/L ratio, COR, MDA, and MPO by about 14, 52, 48, and 29%, respectively, in the infected fish. Furthermore, the mortality rate was reduced by 94%, and the number of pathogenic bacteria cells adherent to the fish gills was lowered by 96% in the infected fish treated with 9 g/kg PR compared to none-PR infected fish. Our results concluded that dietary supplementation with 9 g/kg PR could be a promising nutritional approach for improving the growth performance, physiological profile, and health status of common carp fish, particularly when challenged with F. columnare or similar bacterial infections.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Dietary propolis complementation relieves the physiological and growth deterioration induced by Flavobacterium columnare infection in juveniles of common carp (Cyprinus carpio)

Hassanien,

Alrashada,

Abbas

et al. 2023

PLoS ONE

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Investigating the effects of wild olive Olea europaea leaf extract on growth, body composition, digestive enzyme activity, hematological parameters, and immune function in Nile Tilapia

Naz,

Majeed,

Tasleem

et al. 2024

N American J Aquac

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ObjectiveThis study investigated the effects of supplementing Nile Tilapia Oreochromis niloticus diets with wild olive Olea europaea leaf extract (WLE) on growth performance, digestive enzymes, and antioxidant and immunological parameters.MethodsWild olive leaf extract was added at different concentrations (WLE 0.0 [control], 1.0, 1.5, and 2.0%) and fed to triplicate fish groups for 12 weeks. At the end of the trial period, growth performance, body composition, digestive enzyme activities, and hematological, biochemical, antioxidant, and immunological parameters were evaluated.ResultAccording to the results, fish that were fed with WLE 1.5% and WLE 2.0% exhibited significantly improved weight gain, specific growth rate, and feed intake. The feed conversion ratio (FCR) was significantly reduced in fish supplied with WLE 1.5% and WLE 2.0%. Additionally, weight gain and FCR showed significance at both the linear and quadratic levels, as indicated by polynomial contrasts. However, the experimental diets did not impact fish body composition, digestive enzyme activity, or survival rate. Fish that received WLE 1.5% and WLE 2.0% showed significantly higher levels of white blood cells, red blood cells, hemoglobin, and hematocrit compared with the WLE 1.0% and control groups. Moreover, significant enhancements were observed in serum parameters, including total protein, albumin, globulin, lysozyme, catalase, superoxide dismutase, and total immunoglobulins, for the WLE 1.5% and WLE 2.0% groups compared with the control. Fish that were fed WLE 1.5% exhibited the lowest serum cortisol level.ConclusionIn conclusion, experimental diets with WLE levels of 1.5% and 2.0% effectively enhanced both the growth and immunological parameters of Nile Tilapia.

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Imidacloprid induced growth, hematological, neuro-behavior, anti-oxidant, economic, genetic, and histopathological alterations in Clarias gariepinus: Alleviative role of dietary Hyphaene thebaica

Rahman

AbdelMageed²,

Assayed³

et al. 2023

Aquaculture

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Effect of Olive Leaves and Propolis Extracts on Growth Performance, Immunological Parameters and Economic Efficiency using Nile Tilapia (Oreochromis niloticus)

Cited by 5 publications

References 39 publications

Dietary propolis complementation relieves the physiological and growth deterioration induced by Flavobacterium columnare infection in juveniles of common carp (Cyprinus carpio)

Dietary propolis complementation relieves the physiological and growth deterioration induced by Flavobacterium columnare infection in juveniles of common carp (Cyprinus carpio)

Investigating the effects of wild olive Olea europaea leaf extract on growth, body composition, digestive enzyme activity, hematological parameters, and immune function in Nile Tilapia

Imidacloprid induced growth, hematological, neuro-behavior, anti-oxidant, economic, genetic, and histopathological alterations in Clarias gariepinus: Alleviative role of dietary Hyphaene thebaica

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