Effect of Omeprazole on Intestinal Mucosal Barrier After Hemorrhagic Shock in Rats

Abstract

Objective: The aim of this study was to explain the effect of omeprazole on intestinal mucosal barrier after hemorrhagic shock in rats. Materials and methods: 81 rats were allocated to control, hemorrhagic shock model group and omeprazole group according to random number table, and each group was divided into three subgroups according to the time point of 0, 6, 12 h after HS and resuscitation. The rat model of hemorrhagic shock was induced by using the improved Wiggers method. Model group and omeprazole group were given physiological saline or omeprazole 30 mg/kg retention enema after the post-resuscitation. At three time points, the level of intestinal fatty acid binding protein (IFABP) in the serum was test by ELISA method, and the levels of serum endotoxin (LPS) was detected by dynamic turbidimetric method. The morphological changes of small intestinal mucosa in rats was observed under light microscope, VASP and ZO-1 mRNA expression was measured by RT-qPCR, while p-VASP and ZO-1 protein expression was measured by immunohistochemical method. Results: Compared with model group, serum endotoxin (LPS), fatty acid binding protein (IFABP) content gradually increased (P < 0.05, respectively), and intestinal mucosa damage was aggravate, VASP and ZO-1 gene expression were significantly down-regulation (P < 0.05, respectively) and p-VASP and ZO-1 protein expression were significantly depressed (P < 0.05, respectively). Conclusion: Omeprazole can significantly aggravate the intestinal mucosal barrier function by regulating VAASP and ZO-1 expression after hemorrhagic shock and resuscitation.