Effect of Operating Conditions in Production of Diagnostic Salmonella Enteritidis O-Antigen-Specific Monoclonal Antibody in Different Bioreactor Systems

Ayyildiz-Tamis, Duygu; Nalbantsoy, Ayşe; Elibol, Murat; Deliloglu-Gurhan, S. Ismet

doi:10.1007/s12010-013-0532-4

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…The roller bottles, stirred tank and disposable bioreactors have been operated with both serum and serum-free media. It was found that the cells were not able to adapt to serum-free media in roller bottles and stirred tank bioreactors [ 12 ]. The highest monoclonal antibody titre was observed in a disposable static membrane bioreactor and the peak productivity was found in a stirred tank bioreactor operated in semicontinuous mode with overlay aeration [ 12 ].…”

Section: Track To the Emergence Of High-density Cell Culture Systemsmentioning

confidence: 99%

“…It was found that the cells were not able to adapt to serum-free media in roller bottles and stirred tank bioreactors [ 12 ]. The highest monoclonal antibody titre was observed in a disposable static membrane bioreactor and the peak productivity was found in a stirred tank bioreactor operated in semicontinuous mode with overlay aeration [ 12 ]. Serum free medium has also been successfully used in high cell density bioreactors such as hollow fiber systems [ 18 ], packed bed reactors [ 19 ] and wave bioreactors [ 20 ] for the cultivation of mammalian cells.…”

Section: Track To the Emergence Of High-density Cell Culture Systemsmentioning

confidence: 99%

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Trends in Monoclonal Antibody Production Using Various Bioreactor Syst

Jyothilekshmi

Jayaprakash

2021

J. Microbiol. Biotechnol.

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Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.

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Section: Track To the Emergence Of High-density Cell Culture Systemsmentioning

confidence: 99%

Section: Track To the Emergence Of High-density Cell Culture Systemsmentioning

confidence: 99%

Trends in Monoclonal Antibody Production Using Various Bioreactor Syst

Jyothilekshmi

Jayaprakash

2021

J. Microbiol. Biotechnol.

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“…However, because of ethical concerns and because antibodies have to meet strict quality control requirements (purity, similarity, identity) the number of animals used for antibody production for diagnostic and scientific purposes is continuously decreasing. In parallel, in recent years, numerous bioreactors have been developed for cultivation of hybridoma cells and several types of in vitro antibody production methods were established (Nagel et al 1999;Trebak et al 1999;Ayyildiz-Tamis et al 2014). …”

Section: Introductionmentioning

confidence: 99%

Specific detection and quantitation of bovine IgG in bioreactor derived mouse mAb preparations

Gall-Debreceni¹,

Lázár

Kádas³

et al. 2016

Journal of Immunological Methods

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Monoclonal antibody and recombinant protein production benefits greatly from bovine serum as an additive. The caveat is that bovine serum IgG, co-purifies with mAbs and IgG Fccontaining fusion proteins and it presents a contaminant in the end products. In order to analytically validate the products, species specific reagents are needed that react with bovine IgG exclusively. Our attempts to find such commercially available reagents failed. Here, we report the production of species specific mAbs which recognize bovine IgG even in the presence of excess amount of mouse IgG. We present five mAbs: Bsi4028, Bsi4032, Bsi4033, Bsi4034 and Bsi4035 suitable to determine the presence of bovine IgG contamination via ELISA or immunoblotting in bioreactor derived mouse mAb preparations. To quantitate bovine IgG content we developed sensitive sandwich ELISAs capable to detect bovine IgG contaminant in the ng/ml (~10 -11 M/l) range. Finally, we show that bovine IgG is efficiently removed from bioreactor produced mouse mAb preparation via affinity depletion columns prepared with Bsi4028, Bsi4032, Bsi4033, Bsi4034, Bsi4035 mAbs.

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Production of Monoclonal Antibodies in Serum-free Media

Manna

Febo

Armillotta

et al. 2015

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Four hybridoma cell lines (C4B, 10C2G5, 6C5F4C7, 2D10G11) were adapted to grow in serum-free conditions. Cell proliferation, viability, and antibody production in Nutridoma SP and Ex-Cell HSF 610 media were evaluated and results compared with those obtained using DMEM containing 10% fetal bovine serum (control medium). Clone C4B showed the best IgG productivity in control medium, but viability and number of cells per milliliter were similar for the three media. For clone 10C2G5, the highest values of cell viability were obtained with both control medium and Nutridoma SP; no significant differences in IgG yields and number of cells/mL were observed among the three media. Clone 6C5F4C7 provided no significant differences when grown in the two serum-free media and in control medium. Clone 2D10G11 showed the best IgG productivity in control medium and in Ex-Cell HSF 610, even if Ex-Cell HSF 610 provided the lowest number of cells/mL; no significant differences among the three media were obtained for viability. Purified antibodies produced from each hybridoma cell line grown in serum-free media showed a higher degree of purity than those produced by the same cell lines grown in control medium. Purified MAbs were also titrated by ELISA to test MAb-antigen affinity.

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Effect of Operating Conditions in Production of Diagnostic Salmonella Enteritidis O-Antigen-Specific Monoclonal Antibody in Different Bioreactor Systems

Cited by 4 publications

References 32 publications

Trends in Monoclonal Antibody Production Using Various Bioreactor Syst

Trends in Monoclonal Antibody Production Using Various Bioreactor Syst

Specific detection and quantitation of bovine IgG in bioreactor derived mouse mAb preparations

Production of Monoclonal Antibodies in Serum-free Media

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