Effect of Opiate Receptors Blockade on Microbicidal Potential and Production of IL-1β, TNFα, and IL-10 by Peritoneal Macrophages under Stress Conditions

Abstract

Rotation stress activated spontaneous and zymosan-induced ROS production. In animals receiving naloxone against the background of rotation stress, ROS production did not increase. Immobilization stress did not change the intensity of spontaneous and zymosan-induced ROS production, but inhibited stimulated ROS production against the background of naloxone treatment. Rotation produced a naloxone-independent inhibitory effect on spontaneous and stimulated IL-1β and TNFα production by macrophages and naloxone-dependent stimulating effect on spontaneous IL-10 production. Rotation stress did not modulate stimulated IL-10 production. In case of immobilization stress, decreased IL-1β and TNFα production was observed in mice exposed to stress under conditions of opiate receptors blockade; IL-10 production was not affected by immobilization stress. Both types of stress significantly increased plasma corticosterone levels, while naloxone had no effect on corticosterone production.

Beneficial effects of environmental enrichment on behavior, stress reactivity and synaptophysin/BDNF expression in hippocampus following early life stress

“…Stress was induced by placing the rat cage on a rotating disk (78 revolutions per minute) for a 15 min period. According to existing evidence, cage rotation for durations of 10 or 20 min can produce significant increase in circulating corticosterone concentrations (Gein and Sharavieva, 2016; Riley, 1981). In order to avoid circadian variability, blood sampling was carried out between 0900 and 1100 for all control and experimental groups.…”