Effect of Oral Supplementation of Vitamin E on Urinary Risk Factors in Patients with Hyperoxaluria.

Anbazhagan, Muthusamy; Hariprasad, Chegu; Samudram, P.; Latha, E.; Latha, M. V.; Selvam, Ramasamy

doi:10.3164/jcbn.27.37

Cited by 15 publications

(15 citation statements)

References 23 publications

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“…While reduction (normalization) of plasma TBARS and/or MDA has been observed in hyperoxaluric kidney stone patients [11] and in healthy subjects [39,43], as well as reduction of urinary TBARS in healthy subjects [23,44], similar changes have not been observed in other studies [37,38,45].…”

Section: Discussionmentioning

confidence: 79%

“…We selected this dosage as previous studies using similar levels have detected effects on plasma and urinary risk factors [11,14,22,23]. The duration of the trial was based on two previous scientific studies that investigated the effect of vitamin E (200 mg, 298 IU) on renal stone risk factors in urogenital tuberculosis patients [14,22].…”

Section: Methodsmentioning

confidence: 99%

“…Supplementation also restored the biochemical and kinetic properties of urinary Tamm-Horsfall glycoprotein, a well-known inhibitor of CaOx crystallization. Previous studies have also shown that CaOx stone formers have elevated plasma TBARS [11,15] and NAG [6] levels. In the latter study, it was shown that NAG in stone formers was positively and significantly correlated with their urinary oxalate and calcium.…”

Section: Introductionmentioning

confidence: 98%

“…Indeed, it has been suggested that supplementation with anti-oxidants such as vitamin E, may prevent oxalatemediated peroxidative injury and reduce free radical damage, and hence may prevent CaOx nucleation and retention in the renal tubules [3,11,12]. Thus, vitamin E might be essential to protect molecules and biological systems from oxidative damage [12].…”

Section: Introductionmentioning

confidence: 98%

“…In the latter study, it was shown that NAG in stone formers was positively and significantly correlated with their urinary oxalate and calcium. Moreover, it has been shown that administration of vitamin E reduced urinary excretion of oxalate and calcium in kidney stone patients [11]. All of these parameters are therefore useful indicators of stone risk.…”

Section: Introductionmentioning

confidence: 99%

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Effects of vitamin E ingestion on plasma and urinary risk factors for calcium oxalate urolithiasis in two population groups having different stone-risk profiles: evidence of different physiological handling mechanisms

et al. 2011

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It has been demonstrated that vitamin E supplementation reduces calciuria and oxaluria and that it may also prevent oxalate-mediated peroxidative injury, all of which reduce the risk of calcium oxalate urolithiasis. In view of the significant difference in stone occurrence in black (B) and white (W) South Africans, we undertook to investigate the effects of vitamin E supplementation in subjects from these two groups. Five healthy males from each group ingested one capsule (400 IU) of vitamin E daily for 60 days. Blood and 24 h urine samples were collected at baseline and on day 60; 24 h dietary questionnaires were simultaneously completed. Urine composition was determined by routine analyses. Urinary and plasma TBARS were determined using a commercially available assay kit while plasma vitamin E was determined by reverse phase HPLC. Plasma vitamin E increased significantly in W but not in B. Urinary and plasma TBARS did not increase in either group. Urinary citrate increased significantly in both groups but the percentage increase in W (169%) was greater than that in B (82%). No other urinary parameter changed significantly. The increase in plasma vitamin E in W but not in B suggests either that the mechanism by which it is packaged into chylomicrons, which are secreted into the systemic circulation, is suppressed in the latter group or that it is differentially absorbed in the two groups. Similarly, to explain the greater increase in citraturia in W compared to B, we speculate that inhibition of lipogenesis of arachidonic acid by vitamin E, ultimately leading to an increase in citraturia, occurs to a lesser extent in B than in W.

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Section: Discussionmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of vitamin E ingestion on plasma and urinary risk factors for calcium oxalate urolithiasis in two population groups having different stone-risk profiles: evidence of different physiological handling mechanisms

et al. 2011

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Renal tubular cell damage and oxidative stress in renal stone patients and the effect of potassium citrate treatment

Tungsanga¹,

Sriboonlue²,

Futrakul³

et al. 2004

Urol Res

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Our objective was to evaluate the oxidative stress and renal tubular cell damage in patients who have renal stones compared to normal subjects. The patients were re-evaluated after 1-months supplementation with potassium citrate. We recruited 30 patients (11 males and 19 females) diagnosed with kidney stones and scheduled for surgical stone removal the following month, and 30 healthy non-stone formers (14 males and 16 females). Two 24-h urine samples and one heparinized blood sample were collected from each subject. Plasma was separated from the erythrocytes and assayed for creatinine, potassium, sodium, calcium, magnesium, phosphate, malondialdehyde (MDA, a lipid peroxidation product) (P-MDA), protein thiol as an indicator of protein oxidation, and vitamin E. Erythrocytes were analysed for MDA (E-MDA), reduced glutathione (GSH) and cellular glutathione peroxidase (cGPx) activity. The urine was analyzed for pH, creatinine, potassium, sodium, calcium, magnesium, phosphate, oxalate, citrate, MDA (U-MDA), total protein (U-protein) and N-acetyl-beta-glucosaminidase (NAG) activity. For the stone patients, urine and blood samples were re-evaluated after supplementation with potassium citrate (60 mEq/day) for 1 month. Renal stone patients had higher plasma creatinine and lower plasma potassium, urinary pH, potassium, magnesium, phosphate and citrate than the controls. The patients had higher P-MDA, E-MDA, U-MDA, U-protein and NAG activity, but lower GSH, cGPx activity, protein thiol and vitamin E, when compared with controls. After potassium citrate supplementation, P-MDA and E-MDA decreased while plasma vitamin E, urinary NAG activity and citrate increased. Renal stone disease is associated with high oxidative stress and damage to renal tubular cells. These abnormalities are coincident with an increase in blood lipid peroxidation products and a decrease in antioxidant status. Although supplementation with potassium citrate improved urinary citrate levels and oxidative stress, it neither reduced urinary lipid peroxidation products nor remedied the damage to renal tubular cells, probably due to the existence of kidney stones.

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Examination of the anti-oxidative effect in renal tubular cells and apoptosis by oxidative stress

et al. 2005

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The incidence of urolithiasis has increased in the industrialized nations. However, both the pathogenesis and methods for its prevention remain to be clarified. We demonstrate that the antioxidative effect of green tea decreases the formation of calcium oxalate stones, OPN (osteopontin) expression, and apoptosis, and increases SOD (superoxide dismutase) activity in rat kidney tissues. The inhibitory effect of green tea on calcium oxalate urolithiasis is most likely due to its antioxidative effects. Therefore, we examined oxidative stress in vivo applied to Madin-Darby canine kidney (MDCK) cells, to which catechin, an antioxidant, was added. To evaluate the effects of oxidative stress on MDCK cells, we use a hypoxic condition because hypoxia is known to lead to oxidative stress. Confluent cultures of MDCK cells were exposed to (-)epigallocatechin 3 gallate (EGCG) (0, 0.1, 0.5, 5.0 mg/ml) for 2, 4, 8 or 16 h to determine changes in protein secretion and apoptosis. OPN protein expression was observed in MDCK cells of all 16 groups. The levels of expression of OPN protein were the same among all groups. In all groups, SOD protein expression was observed. In the groups exposed to EGCG 0.5, 5.0 mg/ml, SOD staining was more enhanced than in the EGCG 0 and 0.1 mg/ml groups. No deposits were detected in any of the 16 groups. RT-PCR was performed to detect sequences from OPN (979 bp) and SOD (447 bp). Quantitative analyses showed that SOD activity decreased gradually in all groups. Only in the EGCG 0 mg/ml 16 h group were TUNEL-positive cells observed. In the other groups, TUNEL-positive cells were not detected. EGCG used as an antioxidant protects renal tubular cell from cellular injury caused by oxidative stress through SOD protein expression.

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Effect of Oral Supplementation of Vitamin E on Urinary Risk Factors in Patients with Hyperoxaluria.

Cited by 15 publications

References 23 publications

Effects of vitamin E ingestion on plasma and urinary risk factors for calcium oxalate urolithiasis in two population groups having different stone-risk profiles: evidence of different physiological handling mechanisms

Effects of vitamin E ingestion on plasma and urinary risk factors for calcium oxalate urolithiasis in two population groups having different stone-risk profiles: evidence of different physiological handling mechanisms

Renal tubular cell damage and oxidative stress in renal stone patients and the effect of potassium citrate treatment

Examination of the anti-oxidative effect in renal tubular cells and apoptosis by oxidative stress

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