Effect of orally administered heat-treated Lactobacillus plantarum LM1004 on the innate immune system: A randomized, placebo-controlled, double-blind study

Bae, Won-Young; Min, Hyunggyu; Shin, So Lim; Kim, Tae-Rahk; Lee, Hakbae; Sohn, Minn; Park, Kyoung Sik

doi:10.1016/j.jff.2022.105293

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Previous clinical studies have demonstrated that orally administered probiotics could significantly elevate NK activity in peripheral blood. We noticed that these studies recruited individuals over 60 years old [39,40] or subjects with lower white blood cell counts than usual [41] and had longer intervention periods (≥8 weeks) [42], and their findings were consistent with our previous clinical outcomes [8,22]. No change in NK activity was detected after Lactobacillus casei Shirota intake in healthy male subjects (LcS, n = 34; Control, n = 34) over a 4-week intervention [43].…”

Section: Discussionsupporting

confidence: 82%

The Potential Immunomodulatory Effect of Bifidobacterium longum subsp. longum BB536 on Healthy Adults through Plasmacytoid Dendritic Cell Activation in the Peripheral Blood

Li,

Arai,

Kato

et al. 2023

Nutrients

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The interaction between the gut microbiota and the host can influence the host’s immune system. Bifidobacterium, a commensal genus of gut bacteria, seems to have positive effects on host health. Our previous clinical research showed that B. longum subsp. longum BB536 enhanced innate and adaptive immune responses in elderly individuals with a lower grade of immunity, but the immunomodulatory mechanism is still unclear. In this study, dendritic cell (DC) surface markers in peripheral blood mononuclear cells isolated from healthy individuals were evaluated through coculture with heat-killed BB536. DC markers, innate immune activity and cytokine levels in plasma were also evaluated by a randomized, double-blind, placebo-controlled, parallel-group study (UMIN000045564) with 4 weeks of continuous live BB536 intake. BB536 significantly increased the expression of CD86 and HLA-DR on plasmacytoid DCs (pDCs) in vitro. Compared to placebo (n = 48), a significant increase in the expression of CD86 on peripheral pDCs was detected at week 4 of live BB536 intake (n = 49; 1 × 1010 CFU/day). Furthermore, coculture with hk-BB536 significantly increased the IFNγ expression level and demonstrated trends of increased IFNα1 and IFNβ expression. These findings suggest that consumption of BB536 has potential immunomodulatory effects on healthy individuals through the activation of peripheral pDCs.

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Section: Discussionsupporting

confidence: 82%

The Potential Immunomodulatory Effect of Bifidobacterium longum subsp. longum BB536 on Healthy Adults through Plasmacytoid Dendritic Cell Activation in the Peripheral Blood

Li,

Arai,

Kato

et al. 2023

Nutrients

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“…Heatkilled L. casei IMAU60214 improved immune function by regulating macrophages in malnourished children [35]. When heat-treated L. plantarum LM1004 was consumed for 8 weeks, it stimulated innate immunity and significantly increased NK cells and IL-12 levels [36]. Hirose et al [37] suggested that the daily consumption of heat-killed L. plantarum L-137 increases Th1 immunity and confirmed that it was safe, even when consumed at high doses and for long periods [38].…”

Section: Discussionmentioning

confidence: 99%

Effect of Heat-Treated Lactiplantibacillus plantarum nF1 on the Immune System Including Natural Killer Cell Activity: A Randomized, Placebo-Controlled, Double-Blind Study

Hong,

Lee,

Kim

et al. 2024

Nutrients

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Heat-treated Lactiplantibacillus plantarum nF1 (HT-nF1) increases immune cell activation and the production of various immunomodulators (e.g., interleukin (IL)-12) as well as immunoglobulin (Ig) G, which plays an important role in humoral immunity, and IgA, which activates mucosal immunity. To determine the effect of HT-nF1 intake on improving immune function, a randomized, double-blind, placebo-controlled study was conducted on 100 subjects with normal white blood cell counts. The HT-nF1 group was administered capsules containing 5 × 1011 cells of HT-nF1 once a day for 8 weeks. After 8 weeks of HT-nF1 intake, significant changes in IL-12 were observed in the HT-nF1 group (p = 0.045). In particular, the change in natural killer (NK) cell activity significantly increased in subjects with low secretory (s) IgA (≤49.61 μg/mL) and low NK activity (E:T = 10:1) (≤3.59%). These results suggest that HT-nF1 has no safety issues and improves the innate immune function by regulating T helper (Th)1-related immune factors. Therefore, we confirmed that HT-nF1 not only has a positive effect on regulating the body’s immunity, but it is also a safe material for the human body, which confirms its potential as a functional health food ingredient.

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“…Limosilactobacillus reuteri LM1071 and Streptococcus thermophilus LM1012 have both been reported to have anti-inflammatory effects [ 24 , 25 , 26 ]. Additionally, the immune-boosting effect of a heat-killed material from Lactiplantibacillus plantarum LM1004 has been proven through in vitro and in vivo results, including a human clinical trial [ 27 , 28 , 29 ]. Therefore, the primary goal of the formula was to maintain homeostasis of human immunity with anti-inflammatory and immune-boosting strains.…”

Section: Introductionmentioning

confidence: 99%

Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells

Han¹,

You²,

Cha³

et al. 2023

Microorganisms

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Although leaky gut syndrome is not recognized as an official diagnosis for human diseases, it is now believed that dysfunction of the cell barrier causes increased permeability of intestinal epithelial cells leading to this condition. Probiotics have been widely used to improve gut health, and studies have investigated the relevance of protecting the intestinal barrier by taking probiotic strains in vitro and in vivo. However, most studies have restricted the use of single or several probiotic strains and do not consider commercially available probiotic products composed of multi-species. In this study, we provide experimental evidence that a multi-species probiotic mixture composed of eight different strains and a heat-treated probiotic strain is effective in preventing leaky gut conditions. We employed an in vitro co-culture model system utilizing two different differentiated cell lines to mimic human intestinal tissue. The integrity of epithelial barrier function was protected by the preserving the occludin protein level and activating the AMPK signaling pathway, associated with tight junctions (TJs), through treatment with the probiotic strain mixture in Caco-2 cells. Moreover, we confirmed that application of the multi-species probiotic mixture reduced the expression of proinflammatory cytokine genes by inhibiting NFκB signaling pathway when artificial inflammation was induced in an in vitro co-culture model system. Finally, we proved that the epithelial permeability measured by trans-epithelial electrical resistance (TEER) was significantly decreased in the probiotic mixture treated cells, indicating that the integrity of the epithelial barrier function was not compromised. The multi-species probiotic strain mixture exhibited the protective effect on the integrity of intestinal barrier function via enhancing TJ complexes and reducing inflammatory responses in the human intestinal cells.

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Effect of orally administered heat-treated Lactobacillus plantarum LM1004 on the innate immune system: A randomized, placebo-controlled, double-blind study

Cited by 6 publications

References 14 publications

The Potential Immunomodulatory Effect of Bifidobacterium longum subsp. longum BB536 on Healthy Adults through Plasmacytoid Dendritic Cell Activation in the Peripheral Blood

The Potential Immunomodulatory Effect of Bifidobacterium longum subsp. longum BB536 on Healthy Adults through Plasmacytoid Dendritic Cell Activation in the Peripheral Blood

Effect of Heat-Treated Lactiplantibacillus plantarum nF1 on the Immune System Including Natural Killer Cell Activity: A Randomized, Placebo-Controlled, Double-Blind Study

Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells

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