Effect of Organ Culture on Noradrenaline- Evoked Contraction, Calcium Signalling and TRPC Expression in Rat Mesenteric Artery

Tai, Khalid; Vandenberg, Greet; Hamaide, Marie-Christine; Wibo, Maurice; Morel, Nicole

doi:10.1159/000189796

Cited by 13 publications

(11 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, based on our recent data, TRPC6 protein expression was doubled in siTRPC1-transfected A7r5 VSMCs whose TRPC1 levels decreased 64% by gene silencing (Selli et al 2009). Interestingly, similar interaction was also observed in cultured rat mesenteric artery in which TRPC1 mRNA expression decreased while TRPC6 mRNA expression increased after organ culture (Tai et al 2009). However, more work is required to elucidate whether TRPC6 upregulation is a consequence of TRPC1 downregulation.…”

Section: Discussionsupporting

confidence: 62%

Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders

Erac

Selli

Kosova

et al. 2010

AGE

View full text Add to dashboard Cite

We previously showed that the expression of transient receptor potential canonical (TRPC)6 ion channel elevated when TRPC1 was knocked down in A7r5 cultured vascular smooth muscle cells. Therefore, the purpose of this study was to explore whether TRPC6 is also upregulated in aging rat aorta comparable to that of TRPC1 in longitudinal in vivo aging model. We further investigated a possible causal relationship between altered phenylephrine-induced contractions and the expression levels of TRPC6, a purported essential component of alpha-adrenergic receptor signaling in aging aorta. Immunoblot analysis showed that TRPC1 protein levels significantly decreased whereas TRPC6 increased drastically in aorta from 16- to 20-month-old rats compared to that from 2 to 4 months. Immunohistochemical data demonstrated spatial changes in TRPC6 expression within the smooth muscle layers along with increased detection in the adventitia of the aged rat aorta. The phenylephrine-induced contractions were potentiated in aging aorta. In conclusion, based on this aging model, TRPC6 overexpression could be related with TRPC1 downregulation and might be responsible for the increased adrenoceptor sensitivity which contributes to the development of age-related vasospastic disorders.

show abstract

Section: Discussionsupporting

confidence: 62%

Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders

Erac

Selli

Kosova

et al. 2010

AGE

View full text Add to dashboard Cite

show abstract

“…A previous report showed that this ROCK-dependent calcium entry is not activated by store depletion [9]. The best candidates for driving non-voltage- and non-store-dependent calcium influx are the non-selective cation channels of the TRP canonical family, which have been reported to be expressed in different types of arteries [11,12,26]. By producing sodium and calcium influx, the activation of these channels also causes membrane depolarization and thereby indirectly contributes to the development of the contraction.…”

Section: Discussionmentioning

confidence: 99%

Different Effect of Rho Kinase Inhibition on Calcium Signaling in Rat Isolated Large and Small Arteries

Martinsen¹,

Yerna²,

Rath

et al. 2012

J Vasc Res

Self Cite

View full text Add to dashboard Cite

In addition to its role in the regulation of artery contraction, Rho kinase (ROCK) was reported to be involved in the cytosolic calcium response to vasoconstrictor agonists in rat aorta and superior mesenteric artery (SMA). However, it remains to be determined whether ROCK also contributes to calcium signaling in resistance arteries, which play a major role in blood pressure regulation. The investigation of the effect of ROCK inhibition on the calcium and contractile responses of rat resistance mesenteric artery (RMA), in comparison with aorta and SMA, indicated that the calcium response to noradrenaline was inhibited by the ROCK inhibitor Y-27632 in aorta and SMA but not in RMA. The effect of Y-27632 on the calcium signal was unaffected by cytochalasin-D. ROCK activation in noradrenaline-stimulated arteries was confirmed by the inhibition of myosin light chain phosphorylation by Y-27632. Moreover, noradrenaline-induced calcium signaling was similarly inhibited by nimodipine in aorta, SMA and RMA, but nimodipine sensitivity of the contraction increased from the aorta to the RMA, suggesting that the contraction was controlled by different sources of calcium. In pressurized RMA, Y-27632 and H-1152 depressed pressure-induced calcium responses and abolished myogenic contraction. These results stress the important differences in calcium signaling between conductance and resistance arteries.

show abstract

“…In rat mesenteric arteries cultured for 1 day in the serum-free medium, the upregulation of alpha 1A adrenoreceptor [3] and ET B receptor [7,11,18,19] was reported. With regard to the activation of intercellular signal pathways, a non-receptor tyrosine kinase, src-dependent increase of NA-induced contraction was reported in rat mesenteric arteries cultured in the serumfree condition for 2-3 days [16]. Additionally, it was reported in the same report that the expression of mRNA and protein of TRPC 6 increased after 2-3 days organ culture in the serum-free medium [16].…”

Section: Discussionmentioning

confidence: 93%

“…With regard to the activation of intercellular signal pathways, a non-receptor tyrosine kinase, src-dependent increase of NA-induced contraction was reported in rat mesenteric arteries cultured in the serumfree condition for 2-3 days [16]. Additionally, it was reported in the same report that the expression of mRNA and protein of TRPC 6 increased after 2-3 days organ culture in the serum-free medium [16]. It was also reported in serum-free organ-cultured rat intrapulmonary arteries that the delayed rectifier voltage-gated K -dependent contraction increased [14].…”

Section: Discussionmentioning

confidence: 99%

Contractile Characteristics of Rat Mesenteric Artery after Organ Culture

Morita

Yamawaki

Okada

et al. 2010

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Organ culture of blood vessels is a useful technique to analyze long-term effects of drugs. Various growth factors are responsible for structural and functional changes during vascular remodeling. We investigated 1) basic contractile characteristics in organ-cultured rat mesenteric arteries (MA) in serum-free condition and 2) long-term effects of fetal bovine serum (FBS). Rat isolated MA with [E (+)] or without [E (-)] endothelium were divided into 1) freshly isolated (fresh), 2) cultured for 3 days without FBS (control) or 3) with 10% FBS (FBS). In E (+) control, maximal contraction by noradrenaline (NA), endothelin (ET)-1 and 5-hydroxytriptamine (5-HT) was similar to that in fresh. In E (-) control, maximal contraction by NA decreased whereas that by ET-1 and 5-HT didn't change from fresh. In E (+) FBS, maximal contraction by NA, ET-1, and 5-HT increased from control. In E (-) FBS, maximal contraction by NA and ET-1 decreased whereas that by 5-HT increased. In E (+) or E (-) control and FBS, sensitivity to NA and ET-1 increased from fresh. In E (+) and E (-) control, sensitivity to 5-HT decreased from fresh, and that in FBS further decreased from control. Three-day organ-cultured rat MA in serum-free condition preserved enough contraction to enable analysis for long-term effects of drug. In FBS, maximal contractions by NA and ET-1 increased in E (+) and decreased in E (-) from control, while those by 5-HT increased in both E (+) and E (-).KEY WORDS: blood vessel, cardiovascular pharmacology, contraction, growth factor, smooth muscle.J. Vet. Med. Sci. 72(12): 1621-1627, 2010 In order to sturdy the long-term effects of drugs, animal models have been used extensively. However, due to complicated in vivo kinetics and individual differences, analysis and interpretation of data is often difficult. In contrast, cell culture techniques make it possible to investigate direct effects of drugs and easily control experimental conditions. However, in the cell culture condition, tissue architectures and tissue specific functions are not completely preserved. Furthermore, it is difficult to examine the long-term effects of drugs using cultured vascular cells. Organ culture of blood vessels is thought to be a useful technique to analyze the long-term effects of drugs because it is possible to free from complicated factors existing in vivo and better preserve the differentiated cell functions [21,22].Due to easy handling and management, rats have been used widely in the fields of experimental medicine such as physiology and pharmacology. A rat genome has been clarified, and the most rat genes correspond to those of humans and mice [4]. Since inbred and mutant strains have been established in rats, experiments using rats are reproducible and reliable. However, there are only a few reports that investigated the basic contractile characteristics after longterm organ culture of rat mesenteric artery.Vascular remodeling occurs in cardiovascular disorders such as hypertension, atherosclerosis and diabetes. Various growth f...

show abstract

Effect of Organ Culture on Noradrenaline- Evoked Contraction, Calcium Signalling and TRPC Expression in Rat Mesenteric Artery

Cited by 13 publications

References 72 publications

Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders

Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders

Different Effect of Rho Kinase Inhibition on Calcium Signaling in Rat Isolated Large and Small Arteries

Contractile Characteristics of Rat Mesenteric Artery after Organ Culture

Contact Info

Product

Resources

About