Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’)

Abstract

Dendrobium is a genus of orchids that has been widely planted, both domestically and internationally. Orchid conventional breeding is generally done to produce new variants, but seeds from crosses are generally difficult to germinate. To solve this problem, tissue culture has long been used to germinate orchid seeds. This study aims to obtain a suitable medium composition for growing seeds from crosses of Dendrobium 'Bertachong' X Dendrobium 'Blackspider x Sutiknoi'. The seeds were grown on two different media types, MS (Murashige-Skoog) and VW (Vacin-Went), with the addition of 2% sucrose and with or without the addition of organic extracts (either mungbean sprouts extract or coconut water). Plantlets were sub-cultured every 4 weeks for 2 times on the same medium but with the addition of activated charcoal, and followed by a third subculture with the addition of 1 mg.L -1 NAA (Naphthalene Acetic Acid) in each medium. The best growth shown by fast regeneration and good seedling quality (multiple buds and roots formed) was obtained on MS medium with the addition of 2% sucrose and 15% mungbean sprouts extract.

Multiplication of Dendrobium Sp Orchid Somatic Embries Using In-Vitro Concentrations of MgSO4 and Myo-Inositol in Murashige and Skoog Media

“…Orchid in vitro culture usually uses media supplemented with activated charcoal or carbon (Nguyen et al, 2022;Wijaya et al, 2023) which can absorb toxic compounds in the media or absorb inhibitory compounds secreted by plantlets, stabilize the pH of the media, stimulate root growth by reducing the amount of light entering the media, and stimulate morphogenesis (Chin et al, 2019). In addition, activated charcoal can reduce the browning of the media due to high heat during the sterilization process (Sparjanbabu et al, 2019).…”