Effect of organic carbon sources and environmental factors on cell growth and lipid content of Pavlova lutheri

Bashir, Khawaja Muhammad Imran; Mansoor, Sana; Kim, Nari; Grohmann, Florian Rolando; Shah, Abid Ali; Cho, Man-Gi

doi:10.1007/s13213-018-1423-2

Cited by 19 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our mixotrophic cultures with glycerol did not receive CO 2 , any other source of nutrients (only the inorganic culture medium), or continuous mechanical agitation. In mixotrophic cultures, microalgal growth is affected by CO 2 /O 2 availability from agitation [51]. Despite this, our Yx/s and Yp/s (β-carotene) results are superior to yields obtained by some reports on mixotrophic cultures in this species.…”

Section: Discussioncontrasting

confidence: 96%

Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

2021

View full text Add to dashboard Cite

Current mixotrophic culture systems for Dunaliella salina have technical limitations to achieve high growth and productivity. The purpose of this study was to optimize the mixotrophic conditions imposed by glycerol, light, and salinity that lead to the highest biomass and β-carotene yields in D. salina. The combination of 12.5 mM glycerol, 3.0 M salinity, and 50 μmol photons m−2 s−1 light intensity enabled significant assimilation of glycerol by D. salina and consequently enhanced growth (2.1 × 106 cell mL−1) and β-carotene accumulation (4.43 pg cell−1). The saline and light shock induced the assimilation of glycerol by this microalga. At last stage of growth, the increase in light intensity (300 μmol photons m−2 s−1) caused the β-carotene to reach values higher than 30 pg cell−1 and tripled the β-carotene values obtained from photoautotrophic cultures using the same light intensity. Increasing the salt concentration from 1.5 to 3.0 M NaCl (non-isosmotic salinity) produced higher growth and microalgal β-carotene than the isosmotic salinity 3.0 M NaCl. The mixotrophic strategy developed in this work is evidenced in the metabolic capability of D. salina to use both photosynthesis and organic carbon, viz., glycerol that leads to higher biomass and β-carotene productivity than that of an either phototrophic or heterotrophic process alone. The findings provide insights into the key role of exogenous glycerol with a strategic combination of salinity and light, which evidenced unknown roles of this polyol other than that in osmoregulation, mainly on the growth, pigment accumulation, and carotenogenesis of D. salina.

show abstract

Section: Discussioncontrasting

confidence: 96%

Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

2021

View full text Add to dashboard Cite

show abstract

“…The utilization of organic carbon is species-dependent, and the effects are summarized in Table 2. In an investigation on the effect of organic sources on marine microalgae of Pavlova lutheri, sucrose was identified as prime organic carbon source for growth, followed by glucose, glycerol and acetate (53). Notwithstanding, some microalgae are devoid of metabolize sucrose.…”

Section: Carbonmentioning

confidence: 99%

“…Similar to inorganic carbon, the concentration of organic carbon sources in culture medium must be carefully modulated. Appropriate amount of organic carbon sources can induce microalgal cell growth and metabolites accumulation whereas excessive amount can decline the growth and metabolites accumulation (53). Danesh et al (49) cultivated Isochrysis galbana under different concentration of glycerol.…”

Section: Carbonmentioning

confidence: 99%

Macronutrient effect on biomass of Microalgae in biofuel production: A review

lowast¹,

Tan²,

Wong³

2020

IJST

View full text Add to dashboard Cite

Objectives: This review is focused on the effect of macronutrients (nitrogen, carbon and phosphorus) on biomass production of microalgae especially concerned with biofuel. Methodology: The keyword search included "microalgae cultivation", "nitrogen sources", "phosphorus sources", "organic carbon", "biodiesel", "biofuel", "carbon dioxide", "inorganic carbon", "macronutrient deprivation", "macronutrient limitation", "lipid" and "organic waste" to search the published journals in ScienceDirect, Scopus, Springer, and Google Scholar. The search was performed from December 2019 until Mac 2020 to collect all the journals and books that are published between 2006 and 2020. The effect of each macronutrient (nitrogen, carbon and phosphorus) on microalgal growth of the control and the samples were compared using biomass productivity, concentration and biochemical content in each published article. Findings: Review shows that nitrogen has more pernicious effect than other macronutrients on most microalgal growth and lipid production. The concentrations and types of macronutrients have remarkable effects on the growth of microalgae; hence these criteria must be chosen scrupulously to achieve the desired biomass and metabolite production. In order to improve the biomass and biochemical productivity in concomitant with the cost reduction, replacement of cheap organic waste, genetic engineering of microalgae and two-stage hybrid system have been suggested to simultaneously maximize the biomass and biochemical production. The future research should focus on other biochemical contents such as carbohydrates, proteins and pigment to achieve the biorefinery context which can increase the profit. Besides, economic factor such as factorial design should be included in the future research to obtain the best combined factors with the maximum profit and minimal cost.

show abstract

“…The use of microalgae in the pharmaceutical, medical and food industries is increasing due to their no reported side effects on human health. Thus, it is necessary to investigate microalgae growth parameters and potential growth inhibitors [1]. Heavy metals, antibiotics and herbicides are toxic to microalgae even at low concentrations; however, to a certain extent, microalgae could adapt to a contaminated environment [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae

Bashir¹,

Lee²,

Mansoor

et al. 2021

Energies

Self Cite

View full text Add to dashboard Cite

Heavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium—Cr(VI) on photosynthesis and lipid accumulation in Mucidosphaerium pulchellum and Micractinium pusillum exposed to different concentrations (0–500 μg L−1) was investigated for 11 days. A significant (p < 0.05) increase in lipid content was observed with increasing Cr(VI) concentration. However, growth was suppressed at higher concentrations exceeding 100 μg L−1. Addition of Cr(VI) in the cell culture medium showed a negative effect on quantum yield (Fv/Fm), and a photosynthetic inhibition of >65% was noted in both species at 500 μg L−1. However, the lipid gravimetric analysis presented inner cell lipid content up to 36% and 30% of dry weight biomass for M. pulchellum and M. pusillum, respectively. The fatty acids profiles of both microalgae species showed higher levels of hexadecenoic acid as well as ω3, ω6, and ω7 fatty acids. The effect of Cr(VI) on photosynthesis and lipid accumulation in both microalgae species was concentration and exposure time dependent. This shows that an appropriate concentration of Cr(VI) in culture medium could be beneficial for higher lipid accumulation in freshwater eukaryotic microalgae species.

show abstract

Effect of organic carbon sources and environmental factors on cell growth and lipid content of Pavlova lutheri

Cited by 19 publications

References 45 publications

Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

Macronutrient effect on biomass of Microalgae in biofuel production: A review

The Effect of Chromium on Photosynthesis and Lipid Accumulation in Two Chlorophyte Microalgae

Contact Info

Product

Resources

About