Effect of organic matter on the biodegradation of some organochlorine insecticides in submerged soils

Castro, Teresita F.; Yoshida, Tomio

doi:10.1080/00380768.1974.10432607

Cited by 42 publications

(17 citation statements)

References 7 publications

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“…Cell growth was enhanced in both acclimated and unacclimated cultures, and low concentrations of glucose (0.1 mg l )1 ) stimulated dechlorination. Enhanced degradation of a-, b-, d-and c-HCH was observed in soil with low organic carbon content, when amended with 5000 mg kg )1 glucose under anaerobic conditions (Castro & Yoshida 1974). In another soil with higher organic carbon, the same application level of glucose inhibited HCH removal.…”

Section: Inorganic and Organic Amendmentsmentioning

confidence: 97%

“…Environmental factors affecting biodegradation of HCHs in pure cultures, soil microcosms and field plots HCH degradation under different redox conditions HCH biodegradation was initially thought to be an anaerobic process (Castro & Yoshida 1974;MacRae et al 1967MacRae et al , 1969MacRae et al , 1984Sethunathan et al 1983) and dechlorination of all four commonly found HCH isomers (a, b, d, c) has been observed under anoxic conditions in soil microcosms (Jagnow et al 1977;MacRae et al 1967), soil slurries (MacRae et al 1984Siddaramappa & Sethunathan 1975), field studies involving flooded soils (Chessells et al 1988), pure cultures (Jagnow et al 1977), and in situ in groundwater plumes contaminated with HCHs (Langenhoff et al 2002). Chessells et al (1988) reported a correlation between soil moisture content and removal rates of all four isomers in field agricultural soils treated with an HCH pesticide formulation.…”

Section: Physical Properties and Persistence Of Hchsmentioning

confidence: 99%

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Biodegradation of hexachlorocyclohexane (HCH) by microorganisms

Seech²,

et al. 2005

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The organochlorine pesticide Lindane is the gamma-isomer of hexachlorocyclohexane (HCH). Technical grade Lindane contains a mixture of HCH isomers which include not only gamma-HCH, but also large amounts of predominantly alpha-, beta- and delta-HCH. The physical properties and persistence of each isomer differ because of the different chlorine atom orientations on each molecule (axial or equatorial). However, all four isomers are considered toxic and recalcitrant worldwide pollutants. Biodegradation of HCH has been studied in soil, slurry and culture media but very little information exists on in situ bioremediation of the different isomers including Lindane itself, at full scale. Several soil microorganisms capable of degrading, and utilizing HCH as a carbon source, have been reported. In selected bacterial strains, the genes encoding the enzymes involved in the initial degradation of Lindane have been cloned, sequenced, expressed and the gene products characterized. HCH is biodegradable under both oxic and anoxic conditions, although mineralization is generally observed only in oxic systems. As is found for most organic compounds, HCH degradation in soil occurs at moderate temperatures and at near neutral pH. HCH biodegradation in soil has been reported at both low and high (saturated) moisture contents. Soil texture and organic matter appear to influence degradation presumably by sorption mechanisms and impact on moisture retention, bacterial growth and pH. Most studies report on the biodegradation of relatively low (< 500 mg/kg) concentrations of HCH in soil. Information on the effects of inorganic nutrients, organic carbon sources or other soil amendments is scattered and inconclusive. More in-depth assessments of amendment effects and evaluation of bioremediation protocols, on a large scale, using soil with high HCH concentrations, are needed.

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Section: Inorganic and Organic Amendmentsmentioning

confidence: 97%

Section: Physical Properties and Persistence Of Hchsmentioning

confidence: 99%

Biodegradation of hexachlorocyclohexane (HCH) by microorganisms

Seech²,

et al. 2005

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“…(Sahu et al 1992) and Bacillus brevis and Bacillus circulans (Gupta et al 2000) also degrade the four isomers of HCH. Also, earlier studies indicate that aerobic conditions are more favorable for HCH degradation as reported by Bachmann et al (1988) and Tu (1976) as compared to anaerobic conditions (MacRae et al 1967; Castro and Yoshida 1974). Bacterial strain Kocuria sp.…”

Section: Resultsmentioning

confidence: 76%

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Kumar

Sharma

2016

Bioresour. Bioprocess.

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BackgroundThis study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.MethodsIn this study the enrichment culture method was used for isolation of lindane degrading bacterial isolates, in which the mineral salt medium (MSM) supplemented with different concentrations of lindane was used. Further, the screening for the potential lindane degrading isolates was done using the spray plate method and colorimetric dechlorinase enzyme assay. The selected isolates were also studied for their growth response under varying range of temperature, pH, and NaCl. The finally selected isolates DAB-1Y and DAB-1W showing best lindane degradation activity was further subjected to biochemical characterization, microscopy, degradation/kinetic study, and 16S rDNA sequencing. The strain identification were performed using the biochemical characterization, microscopy and the species identifies by 16S rDNA sequence of the two isolates using the standard 16S primers, the 16 S rRNA partial sequence was analyzed through BLAST analysis and phylogenetic tree was generated based on UGPMA clustering method using MEGA7 software. This shows the phylogenetic relationship with the related strains. The two isolates of this study were finally characterized as Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W, and their 16S rRNA sequence was submitted to GenBank database with accession numbers, KJ811539 and KX986577, respectively.ResultsOut of the 20 isolates, the isolates DAB-1Y and DAB-1W exhibited best lindane-degrading activity of 94 and 98%, respectively, recorded after 8 days of incubation. The optimum growth was observed at temperature 30 °C, pH 7, and 5% NaCl observed for both isolates. Of the four isomers of hexachlorocyclohexane, isomer α and γ were the fastest degrading isomers, which were degraded up to 86 and 94% by isolates DAB-1Y and up to 93 and 98% by DAB-1W, respectively, reported after 8 days incubation. Isomer β was highly recalcitrant in which maximum 35 and 32% lindane degradation was observed even after 28 days incubation by isolates, DAB-1Y and DAB-1W, respectively. At lower lindane concentrations (1–10 mg/L), specific growth rate increased with increase in lindane concentration, maximum being 0.008 and 0.006/day for DAB-1Y and DAB-1W, respectively. The 16 S rRNA partial sequence of isolate DAB-1Y showed similarity with Kocuria sp. by BLAST analysis and was named as Kocuria sp. DAB-1Y and DAB-IW with Staphylococcus sp. DAB-1W. The 16S rDNA sequence of isolate DAB-1Y and DAB-1W was submitted to online at National Centre of Biotechnology Information (NCBI) with GenBank accession numbers, KJ811539 and KX986577, respectively.ConclusionsThis study has demonstrated that Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W were found efficient in bioremediation of gamma-HCH and can be utilized further for biodegradation of environmental contamination of lindane and can be utilized in bioremediation program.Electronic supplement...

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“…Repression of HCH biodegradation by glucose and other carbon amendments has been reported for aerobic soil slurries [2] and stirred anaerobic cultures [18]. However, other reports have indicated that carbon amendment might enhance HCH removal, depending on the nutrient requirements of the degrading microorganisms and the initial carbon content of the soil [9,12,17], or by promoting lower redox potentials and enhancing anaerobic degradation rates [26]. In this study, the extraction data demonstrated that HCH removal (without mineralization) was equal in treatments 4 and 6 by day 275 (Fig.…”

Section: Hch Degradation and Mineralization Of 14 C-c-hchmentioning

confidence: 99%

Mineralization of hexachlorocyclohexane in soil during solid-phase bioremediation

Phillips¹,

Lee²,

Trevors³

et al. 2004

J IND MICROBIOL BIOTECHNOL

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Soil containing hexachlorocyclohexane (HCH) was spiked with (14)C-gamma-HCH and then subjected to bioremediation in bench-scale microcosms to determine the rate and extent of mineralization of the (14)C-labeled HCH to (14)CO(2). The soil was treated using two different DARAMEND amendments, D6386 and D6390. The amendments were previously found to enhance natural HCH bioremediation as determined by measuring the disappearance of parent compounds under either strictly oxic conditions (D6386), or cycled anoxic/oxic conditions (D6390). Within 80 days of the initiation of treatment, mineralization was observed in all of the strictly oxic microcosms. However, mineralization was negligible in the cycled anoxic/oxic microcosms throughout the 275-day study, even after cycling was ceased at 84 days and although significant removal (up to 51%) of indigenous gamma-HCH (146 mg/kg) was detected by GC with electron capture detector. Of the amended, strictly oxic treatments, only one, in which 47% of the spiked (14)C-HCH was recovered as (14)CO(2), enhanced mineralization compared with an unamended treatment (in which 34% recovery was measured). Other oxic treatments involving higher amendment application rates or auxiliary carbon sources were inhibitory to mineralization. Thus, although HCH degradation occurs during the application of either oxic or cycled anoxic/oxic DARAMEND treatments, mineralization of gamma-HCH may be inhibited depending on the amendment and treatment protocol.

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Effect of organic matter on the biodegradation of some organochlorine insecticides in submerged soils

Cited by 42 publications

References 7 publications

Biodegradation of hexachlorocyclohexane (HCH) by microorganisms

Biodegradation of hexachlorocyclohexane (HCH) by microorganisms

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Mineralization of hexachlorocyclohexane in soil during solid-phase bioremediation

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