2019
DOI: 10.1016/j.procbio.2019.03.009
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Effect of osmolytes on the activity of anti-cancer enzyme L-Asparaginase II from Erwinia chrysanthemi

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Cited by 20 publications
(6 citation statements)
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“…As tagged proteins are normally used in single‐step affinity purification and automated CFPS processes, 26‐28 an L‐ASNase enzyme with a His‐tag fused at the N‐terminal position – named L‐ASNase (I) – was expressed. In fact, a previous study has shown no significant loss of L‐ASNase activity when the recombinant protein was fused to a His‐tag at the N‐terminus 29 . Other L‐ASNase genetic constructs were also tested for expression in this CFPS system: (II) L‐ASNase with no His‐tag, (III) L‐ASNase with a His‐tag at the C‐terminus, and an (IV) L‐ASNase with a His‐tag at the N‐terminus, which contained a different protease cleavage site between the His‐tag and the L‐ASNase protein sequence in comparison to L‐ASNase (I) (Fig.…”
Section: Resultsmentioning
confidence: 95%
“…As tagged proteins are normally used in single‐step affinity purification and automated CFPS processes, 26‐28 an L‐ASNase enzyme with a His‐tag fused at the N‐terminal position – named L‐ASNase (I) – was expressed. In fact, a previous study has shown no significant loss of L‐ASNase activity when the recombinant protein was fused to a His‐tag at the N‐terminus 29 . Other L‐ASNase genetic constructs were also tested for expression in this CFPS system: (II) L‐ASNase with no His‐tag, (III) L‐ASNase with a His‐tag at the C‐terminus, and an (IV) L‐ASNase with a His‐tag at the N‐terminus, which contained a different protease cleavage site between the His‐tag and the L‐ASNase protein sequence in comparison to L‐ASNase (I) (Fig.…”
Section: Resultsmentioning
confidence: 95%
“…4 b and c). Therefore, the protein disaggregation can be explained by the favorite hydration of the enzyme after mixing with osmolyte, which produces intra-chain bonds that can favor equilibrium in the direction of active conformations [ 50 ].…”
Section: Resultsmentioning
confidence: 99%
“…helps stabilizing enzymes [29][30][31][32]. Protective effect of polyols was reported in many recent studies in different experimental situations and exposition to stresses (high pressure or high temperature, presence of non-aqueous solvents) [33][34][35][36][37].…”
Section: Chemical Stabilizer Additionmentioning
confidence: 89%