Effect of osmotic shock on the redox system in plasma membrane of Dunaliella salina

Abstract

The unicellular halotolerant alga Dunaliella salina had the ability to oxidize NADH and reduce Fe(CN) 6 3-. The redox reactions were to some extent stimulated by slight hyperosmotic shock (2.0 mol/L → 2.6 mol/L NaC1), but markably inhibited by abrupt hyperosmotic shock (2.0 mol/L → 3.5 mol/L NaC1) and hypoosmotic shock (2.0 mol/L → 1.0 mol/L NaC1; 2.0 mol/L→0.67 mol/L NaC1).With the adaptation of algal cells to osmotic shock by accumulating or degrading intracellular glycerol, the plasmalemma redox activities were also restored.

The Mechanosensitive Ion Channel MSL10 Potentiates Responses to Cell Swelling in Arabidopsis Seedlings

“…Data are reported as relative fold change (fluorescence intensity of treated root)/ (fluorescence intensity of control root), to account for mechanical perturbations that occurred during the assay. For ROS inhibition, seedlings were incubated with ROS inhibitors or scavengers, including KI (10 mM), DPI (10 mM) and SHAM (50 mM) as previously described [18,23,109]. These chemicals were added during the 4-hour equilibration step before seedlings were subjected to iso-or hypo-osmotic treatments.…”