Effect of osmotic shrinkage and hormones on the expression of Na+/H+ exchanger-1,Na+/K+/2Cl– cotransporter and Na+/K+-ATPase in gill pavement cells of freshwater adapted Japanese eel,<i>Anguilla japonica</i>

Tse, William Ka Fai; Au, Doris W.T.; Wong, Chris K C

doi:10.1242/jeb.004101

Cited by 44 publications

(28 citation statements)

References 60 publications

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“…Our previous study demonstrated hypertonic-induced cell shrinkage, RVI and the expression of Ostf (Tse et al, 2007;Tse et al, 2008). In this study, using immunocytochemical staining, our data indicate that hypertonic treatment induced an increase in F-actin staining at the periphery of the hypertonic-treated gill cells.…”

Section: Discussionsupporting

confidence: 70%

“…Gill arches were excised and some gill filaments were directly dissolved in Trizol solution for total RNA extraction or fixed in 4% paraformaldehyde (PFA, Sigma, St Louis, MO, USA) for immunohistochemistry. Other gill tissues were cut into small fragments and were subjected to two cycles of tryptic digestion (0.5% trypsin+5.3 mmol l -1 EDTA) for the establishment of primary gill cell culture as previously described (Tse et al, 2007;Tse et al, 2008). After tryptic digestion, the cell suspension was filtered and washed.…”

Section: Animals and Gill Cell Isolationmentioning

confidence: 99%

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Cloning and regulation of expression of the Na+–Cl––taurine transporter in gill cells of freshwater Japanese eels

Chow

Ching

Wong

et al. 2009

Journal of Experimental Biology

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SUMMARYOur previous studies have demonstrated the hypertonic-induced expression of osmotic stress transcription factor and the regulatory volume increase (RVI) response in gill cells isolated from freshwater eels. In this study, we aimed to clone one of the organic osmolyte transporters, the Na + -Cl --taurine transporter (TauT), and to characterize its expression in anisosmotic conditions, using both in vivo and in vitro approaches. A cDNA clone encoding TauT was isolated from gill tissues of Japanese eels, Anguilla japonica. The deduced amino acid sequence shows 88-90% identity to other reported piscine TauT sequences. Our data indicated that TauT mRNA was detectable in both freshwater and seawater fish gills. The expression level of TauT mRNA increased in gills of seawater-acclimating fish. A high abundance of TauT protein was found to be localized in seawater gill chloride cells. Using primary gill cell culture, expression of the gene was induced when the ambient osmolarity was raised from 320 to 500 mosmol l Supplementary material available online at

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Section: Discussionsupporting

confidence: 70%

Section: Animals and Gill Cell Isolationmentioning

confidence: 99%

Cloning and regulation of expression of the Na+–Cl––taurine transporter in gill cells of freshwater Japanese eels

Chow

Ching

Wong

et al. 2009

Journal of Experimental Biology

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“…These modulations of gene expression occurred in response to plasma osmotic conditions routinely experienced during the acute phases of FW (~280 mOsm/kg) and SW (~450 mOsm/kg) acclimation (Inokuchi et al 2015). Hyperosmotic conditions stimulate the gene expression of Na + /K + /2Cl − cotransporter 1a (nkcc1a), a key cotransporter underlying ion extrusion by teleost ionocytes , Inokuchi et al 2015, as well as volume regulation by pavement cells (Tse et al 2007). Thus, enhanced aqp3/Aqp3 expression in the basolateral region of branchial cells in FW-acclimated tilapia (Fig.…”

Section: :3mentioning

confidence: 99%

“…The evolved capacities of these 'euryhaline' fishes to tolerate disparate salinities necessitate that epithelial surfaces in direct contact with external conditions possess sensory systems that detect changes in ambient osmolality (Fiol & Kültz 2007). These osmosensory systems couple with regulatory systems that both protect cellular homeostasis and modulate the expression of ion transport effectors responsible for maintaining homeostasis of the organism (Fiol & Kültz 2007, Tse et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Hormonal regulation of aquaporin 3: opposing actions of prolactin and cortisol in tilapia gill

Breves

Inokuchi

Yamaguchi

et al. 2016

Journal of Endocrinology

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Aquaporins (Aqps) are expressed within key osmoregulatory tissues where they mediate the movement of water and selected solutes across cell membranes. We leveraged the functional plasticity of Mozambique tilapia (Oreochromis mossambicus) gill epithelium to examine how Aqp3, an aquaglyceroporin, is regulated in response to osmoregulatory demands. Particular attention was paid to the actions of critical osmoregulatory hormones, namely, prolactin (Prl), growth hormone and cortisol. Branchial aqp3 mRNA levels were modulated following changes in environmental salinity, with enhanced aqp3 mRNA expression upon transfer from seawater to freshwater (FW). Accordingly, extensive Aqp3 immunoreactivity was localized to cell membranes of branchial epithelium in FW-acclimated animals. Upon transferring hypophysectomized tilapia to FW, we identified that a pituitary factor(s) is required for Aqp3 expression in FW. Replacement with ovine Prl (oPrl) was sufficient to stimulate Aqp3 expression in hypophysectomized animals held in FW, an effect blocked by coinjection with cortisol. Both oPrl and native tilapia Prls (tPrl 177 and tPrl 188 ) stimulated aqp3 in incubated gill filaments in a concentration-related manner. Consistent with in vivo responses, coincubation with cortisol blocked oPrl-stimulated aqp3 expression in vitro. Our data indicate that Prl and cortisol act directly upon branchial epithelium to regulate Aqp3 in tilapia. Thus, within the context of the diverse actions of Prl on hydromineral balance in vertebrates, we define a new role for Prl as a regulator of Aqp expression.

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“…The results of recent research underline the importance of the physiological role of the described mechanism, directly conjugated with the basic homeostatic functions of the gill apparatus, ensuring the stability of the parameters of gas exchange, acid-base and osmotic balance of internal environment (Evans et al 2005). However, some authors ascertain the relevance of further research, whose aim is, firstly, the creation of adequate models of clearance of ammonia, which is closely associated with the work of the ion-transporting systems of gill epithelium (Wright and Wood Ch 2009), in-Second, the analysis of physiological and evolutionary aspects of the ways renal clearance of ammonia in fish (Nakada et al 2007), as well as to study the features of neuro-humoral control systems of homeostatic functions of the gill apparatus of fish (Hoshijima and Hirose Sh 2007;Tse et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Features of nitrogen metabolism in fishes

Dolomatov

Shekk

Zukow

et al. 2011

Rev Fish Biol Fisheries

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Presence in water is easily metabolized nitrogen-containing substances is a vital condition for the existence of food organisms in ponds used for fish reproduction. At the same time, the end products of nitrogen metabolism-ammonia, nitrites and nitrates are always displayed the body of fish into the environment, adding to the total number of these compounds dissolved in water. Ammonia, nitrite and nitrate anions are formed during biological processes, as well as man-made origin, have toxic properties and are considered as environmental factors that may limit fish productivity of reservoirs. The results of a brief review of the literature showed that the features of nitrogen metabolism in fish are actual direction of research, both theoretical science and in practical fish farming. Published the results of studies of amino acid metabolism in fish suggest that, firstly, the processes responsible for the neutralization and the release of ammonia from the body of fish, as well as ontogenetic aspects of the dynamics of formation and emission of ammonia is not fully understood. Meanwhile, several authors stressed the importance of this area of interest for the formation of science-based ways to improve the efficiency of plant breeding in fish culture. Secondly, endogenous nitrite, being the product of oxidation of the molecule nitric oxide and its transport form, the norm are always present in fish. However, the mechanisms responsible for maintaining the stability of the concentration of nitrite in the internal environment of fish, not well understood. Also, in our opinion, of interest to determine the physiological rules of nitrites and nitrates in the body of various species, depending on the seasons and stages of ontogeny. Thirdly, the literature pays much attention to the toxic properties of nitrite in water biocenosis. Certainly, the analysis of the manifestation in fish tissue-specific toxic effects of nitrites and their derivatives-an important area of research. Nevertheless, we can not exclude that it may be quite promising analysis of the incorporation of exogenous nitrite as a substrate exist in fish of the physiological mechanisms re-synthesis of nitric oxide. This kind of ''interference'' of endogenous and exogenous nitrite-anions are not causing serious structural damage to organs and tissues, however, can cause substantial damage to the practical fish farming, causing changes in the level of regulatory mechanisms of fish at the most critical stages: when working with manufacturers, the incubation of eggs, and larvae rearing and wintering fish.

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Effect of osmotic shrinkage and hormones on the expression of Na+/H+ exchanger-1,Na+/K+/2Cl– cotransporter and Na+/K+-ATPase in gill pavement cells of freshwater adapted Japanese eel,Anguilla japonica

Abstract: the regulation is rapid and is probably induced via nongenomic actions.

Cited by 44 publications

References 60 publications

Cloning and regulation of expression of the Na+–Cl––taurine transporter in gill cells of freshwater Japanese eels

Cloning and regulation of expression of the Na+–Cl––taurine transporter in gill cells of freshwater Japanese eels

Hormonal regulation of aquaporin 3: opposing actions of prolactin and cortisol in tilapia gill

Features of nitrogen metabolism in fishes

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