Effect of ouabain on myocardial remodeling in rats

Ren, Yanping; Zhang, Mingjuan; Zhang, Ting; Huang, Ruowen

doi:10.3892/etm.2013.1098

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…The doses infused were estimated to increase the plasma ouabain concentration by 0.5–1.0 nmol/l above the normal physiological level (approximately 0.3–0.9 nmol/L) [ 29 ], thus leading to an ouabain concentration similar to that in pathological conditions such as hypertension and heart failure (approximately 0.9–1.8 nmol/L) [ 30 ]. Intraperitoneal injection of ouabain daily for consecutive eight weeks has been proven to effectively increase blood pressure in SD rats [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Intracerebroventricular injection of antibody Fab fragments against ouabain could effectively inhibit excess sympathetic activation and prevent the elevation of blood pressure in Dahl salt-sensitive hypertensive rats [ 17 ]. Hypertensive animal models can be established through intraperitoneal injection of ouabain [ 18 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, ouabain could also induce cardiac remodeling in rats independent of blood pressure [ 21 ]. The proliferation of collagen fibers among myocardial cells, as well as mitochondrial proliferation and swelling in myocardial cells, was observed under electron microscopy in ouabain-treated rats [ 22 ] without obviously elevated blood pressure [ 21 ]. Mechanisms involved in ouabain-induced cardiac remodeling have not been completely elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Renal Denervation on Ouabain‐Induced Hypertension in Rats

Tang,

Hu,

et al. 2024

International Journal of Hypertension

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Background. Ouabain, a Na+, K+‐ATPase inhibitor, is elevated in hypertensive patients. Evidence suggests ouabain contributes to hypertension mainly through activation of the sympathetic nervous system (SNS). Renal nerves play a vital role in the regulation of SNS activity, so we hypothesize that renal denervation may attenuate the development of ouabain‐induced hypertension. Methods and Results. Forty Sprague‐Dawley rats were divided into following groups (n = 10 each): control group (sham surgery plus intraperitoneal saline injection), RDN group (renal denervation (RDN) plus intraperitoneal saline injection), ouabain group (sham surgery plus intraperitoneal ouabain injection), and ouabain + RDN group (RDN plus intraperitoneal ouabain injection). After eight weeks, compared with the control group, rats in the ouabain group exhibited elevated blood pressure (P < 0.05), increased plasma epinephrine, norepinephrine, angiotensin II, and aldosterone levels (P < 0.05). These indexes could be significantly ameliorated by RDN. RDN also reduced the thickening of aortic tunica media and downregulated the expression of proliferating cell nuclear antigen (PCNA) in the thoracic aorta induced by ouabain. Masson staining and echocardiography showed that myocardial fibrosis and increased left ventricular mass in the ouabain group could be attenuated by RDN. Conclusions. The present study reveals that renal nerves play an important role in the development of ouabain‐induced hypertension. RDN could inhibit the pressor effect and the myocardial remodeling induced by ouabain potentially via inhibiting catecholamine release and vascular smooth muscle cell proliferation. Clinical studies are needed to explore whether RDN may exhibit better antihypertensive effects on hypertensive patients with high plasma ouabain levels as compared to those with normal plasma ouabain levels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Renal Denervation on Ouabain‐Induced Hypertension in Rats

Tang,

Hu,

et al. 2024

International Journal of Hypertension

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“…Several lines of evidence suggest that endogenous ouabain could be involved in the pathogenesis of essential hypertension. It affects the function of all cells by binding to Na + /K + –ATPase (Meira et al , 2014; Miles et al , 2013) and associated with increased sympathetic activity (Pavlov et al , 2005; Ren et al , 2013). Moreover, it induces activation of the brain renin-angiotensin-aldosterone system and promotes increases in the activity of endothelial angiotensin-converting enzyme (ACE) and the local synthesis of angiotensin II (Ketchem et al , 2016; Padilha et al , 2011).…”

Section: Discussionmentioning

confidence: 99%

Flaxseeds (L. Usitatissimum) attenuates blood pressure, acetylcholinesterase activity and oxidative stress in ouabain-induced hypertension in normal Wistar rats

Berzou

Krouf

Taleb-Dida

et al. 2019

NFS

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Purpose The purpose of this study is to evaluate the effects of flaxseed (L. usitatissimum [Linn]) on blood pressure, redox status markers and acetylcholinesterase enzyme activity in ouabain-induced hypertension in normal Wistar rats. Design/methodology/approach Male Wistar rats weighing 250 ± 7 g (n = 24) fed with an experimental diet containing 20 per cent casein were divided into three groups (n = 8) and received a daily subcutaneous injection of either 0.9 per cent saline solution (T group) or 10 µg/kg/day of ouabain diluted in saline solution-treated Oub-Lu or not with 1 per cent of flaxseed (L. usitatissimum) mixed in the diet for 21 days. Findings The results showed that treatment with flaxseed had a significant effect (p < 0.05) in decreasing systolic, diastolic blood pressure, mean arterial pressure and the heart rate in hypertensive rats. Total and non-HDL cholesterol levels were reduced by –26 and –35 per cent; p = 0.04, respectively. Concentrations of thiobarbituric acid-reactive substances were significantly decreased by –85 and –42 per cent (p = 0.001 and p = 0.04), respectively in the plasma and heart. Nitric oxide levels were increased in the aorta (+ 63 per cent; p = 0.001). Moreover, in the heart and aorta, a significant increase was noted in the thiol contents (+81 and +69 per cent; p = 0.001, respectively), glutathione peroxidase (+50 per cent; p = 0.03 and p = 0.01, respectively) and acetylcholinesterase activities (75 and +19 per cent, respectively; p = 0.001 and p = 0.04). Originality/value These results suggest hypotensive, cardiomoderating and antioxidant effects of flaxseed in ouabain-induced hypertension in the rat. In addition, it promotes a significant increase of the acetylcholinesterase activity in tissues.

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“…Changes in ion currents, including Ca 2+ and K + , form the basis for early electrical remodeling in AF (8,9). Calcium entry from the extracellular space through LTCCs and the resultant intracellular Ca 2+ elevation (calcium overload) was demonstrated to be crucial in the regulation of atrial frequency-dependent action potential duration (APD) and effective refractory period (ERP) (10). The transient outward K + current mediated by the potassium channel Kv4.3 contributes to early repolarization (11).…”

Section: Introductionmentioning

confidence: 99%

Nkx2.5/CARP signaling pathway contributes to the regulation of ion channel remodeling induced by rapid pacing in rat atrial myocytes

Wang

Zhu

et al. 2016

Molecular Medicine Reports

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Remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (AF). Evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including Nkx2.5, are crucial for the development of AF. Rat atrial myocardial cells (AMCs) in culture dishes were placed in an electric field and stimulated. Transmission electron microscopy was used to observe the ultrastucture prior to and following rapid pacing. The action potential durations and effective refractory periods were measured. RT‑PCR and western blotting were performed to investigate the effect of rapid pacing on the expression levels of ion channel and nuclear proteins in AMCs. Nkx2.5 short interfering RNA (siRNA) transfection was performed. Through this in vitro rat AMC culture and rapid pacing model, it was demonstrated that rapid pacing shortened the action potential and downregulated the expression levels of L‑type calcium and potassium channels. Expression levels of Nkx2.5 and cardiac ankyrin repeat protein (CARP) were significantly upregulated by rapid pacing at the mRNA and protein levels. siRNA‑mediated Nkx2.5 silencing attenuated the rapid pacing‑induced downreglation of ion channel levels. These results suggest that the Nkx2.5/CARP signaling pathway contributes to the early electrical remodeling process of AF.

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Effect of ouabain on myocardial remodeling in rats

Cited by 4 publications

References 31 publications

Effects of Renal Denervation on Ouabain‐Induced Hypertension in Rats

Effects of Renal Denervation on Ouabain‐Induced Hypertension in Rats

Flaxseeds (L. Usitatissimum) attenuates blood pressure, acetylcholinesterase activity and oxidative stress in ouabain-induced hypertension in normal Wistar rats

Nkx2.5/CARP signaling pathway contributes to the regulation of ion channel remodeling induced by rapid pacing in rat atrial myocytes

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