Effect of ovarian hormones on the healthy equine uterus: a global gene expression analysis

Marth, C; Young, Neil D.; Glenton, Lisa Y.; Noden, Drew M.; Browning, Glenn F.; Krekeler, Natali

doi:10.1071/rd14513

Cited by 8 publications

(3 citation statements)

References 73 publications

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“…These data are detailed in Table 1 and processed using the same pipeline as our generated data, as described subsequently. Publications from these data include Coleman et al 2013, Fushan et al 2015, Marth et al 2015, Mansour et al 2017, Burns et al 2018, and Janecka et al 2018.…”

Section: Sequence Read Archivementioning

confidence: 99%

New insights in equine steroidogenesis: an in-depth look at steroid signaling in the placenta

et al. 2020

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Steroid production varies widely among species, with these differences becoming more pronounced during pregnancy. As a result, each species has its own distinct pattern of steroids, steroidogenic enzymes, receptors, and transporters to support its individual physiological requirements. Although the circulating steroid profile is well characterized during equine pregnancy, there is much yet to be explored regarding the factors that support steroidogenesis and steroid signaling. To obtain a holistic view of steroid-related transcripts, we sequenced chorioallantois (45 days, 4 months, 6 months, 10 months, 11 months, and post-partum) and endometrium (4 months, 6 months, 10 months, 11 months, and diestrus) throughout gestation, then looked in-depth at transcripts related to steroid synthesis, conjugation, transportation, and signaling. Key findings include: 1) differential expression of HSD17B isoforms among tissues (HSD17B1 high in the chorioallantois, while HSD17B2 is the dominant form in the endometrium) 2) a novel isoform with homology to SULT1A1 is the predominant sulfotransferase transcript in the chorioallantois; and 3) nuclear estrogen (ESR1, ESR2) and progesterone (PGR) expression is minimal to nonexistant in the chorioallantois and pregnant endometrium. Additionally, several hypotheses have been formed, including the possibility that the 45-day chorioallantois is able to synthesize steroids de novo from acetate and that horses utilize glucuronidation to clear estrogens from the endometrium during estrous, but not during pregnancy. In summary, these findings represent an in-depth look at equine steroid-related transcripts through gestation, providing novel hypotheses and future directions for equine endocrine research.

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Section: Sequence Read Archivementioning

confidence: 99%

New insights in equine steroidogenesis: an in-depth look at steroid signaling in the placenta

et al. 2020

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“…Due to the increased chance of contamination during oestrus, it is expected that the equine endometrium exhibits a more efficient immune defence response. In fact, Marth et al () reported upregulation of genes involved in the endometrial immune response of mares during oestrus.…”

Section: Introductionmentioning

confidence: 99%

A comparison of the uterine proteome of mares in oestrus and dioestrus

Maloney

Khan

Chenier

et al. 2018

Reprod Domestic Animals

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Contents Proteomic analysis of mare uterine flush fluid provides a minimally invasive technique for studying protein changes associated with the oestrous cycle. The aim of this study was to identify differentially abundant proteins in the uterine flush fluid of mares in oestrus and dioestrus. In this study, uterine flush fluid samples were collected from eight reproductively healthy mares in either oestrus (n = 5) or dioestrus (n = 3). Proteomic analysis was performed using liquid chromatography‐tandem mass spectrometry. Of 172 proteins identified, six proteins (immunoglobulin lambda‐like polypeptide 1, haemoglobin subunit alpha, alpha‐1B‐glycoprotein, serotransferrin, apolipoprotein A‐1, and haemoglobin subunit beta) were significantly more abundant in oestrus. These proteins may contribute to the endometrial defence system through roles in inflammation, immunity or antimicrobial activity. In other species, some of these proteins have been described as immunoglobulins, negative acute phase proteins or defence agents against micro‐organisms. During dioestrus, immunoglobulin alpha‐1 chain C region‐related, complement factor I, CD 109 antigen and uterocalin, were significantly more abundant. Research in other species suggests that these four proteins contribute to the immune response through proposed immunoregulatory characteristics, complement system involvement or roles in B cell–T cell interactions. In conclusion, ten differentially abundant proteins were identified in the uterine flush fluid of mares in oestrus and dioestrus. Targeted studies on these proteins could elucidate their role in uterine defence mechanisms during the oestrous cycle in the mare.

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“…A previous next-generation sequencing study found that several pathways related to the immune response were enriched in the oestrus gene expression profiles of cycling mares when compared to their dioestrus profiles, suggesting increased immunological vigilance during oestrus [16]. Inoculation of E. coli into the uterus of healthy mares during oestrus [7] results in a much less pronounced inflammation than in dioestrus [17], in which purulent or serohaemorrhagic vaginal discharge, heavy bacterial growth and acute systemic inflammation were detected.…”

Section: Introductionmentioning

confidence: 99%

Oestrous cycle-dependent equine uterine immune response to induced infectious endometritis

Marth

Firestone

Glenton

et al. 2016

Vet Res

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Infectious endometritis is a major cause of reduced pregnancy rates in horses. The objectives of this study were to establish a timeline of the innate immune response in the uterus of healthy horses and to investigate the oestrous cycle effect on this. Endometrial biopsies were collected from five horses before and at 3, 12, 24, 48 and 72 h after inoculation of Escherichia coli, once in oestrus and once in dioestrus. They were analysed by quantitative real-time PCR, microbiology and histology. Neutrophil numbers increased from very low levels in the absence of inflammation to severe neutrophilia 3 h after inoculation. The concentrations of mRNAs for Toll-like receptor (TLR)2, TLR4, NOD-like receptor NLRC5, tissue inhibitor of metallopeptidases 1 (TIMP1) and chemokines CCL2, CXCL9, CXCL10 and CXCL11 were all increased 3 h after inoculation of E. coli compared to levels detected prior to inoculation. Chemokine mRNA levels remained elevated for 48 h. Concentrations of mRNAs for the antimicrobial peptides equine β-defensin 1 (EBD1), lysozyme, secretory leukoprotease inhibitor (SLPI), lipocalin 2 (LCN2), lactoferrin and uteroferrin were increased between 3 and 12 h post inoculation. The gene for secreted phospholipase A2 (sPLA2) was expressed constitutively. P19 uterocalin mRNA levels were higher in dioestrus than in oestrus over the first 24 h of inflammation. Neutrophils and many innate immune genes responded rapidly to the introduction of E. coli into the uterus, while the oestrous cycle stage had only a relatively minor effect on the response to E. coli. This study has delineated a useful model of innate immunity in infectious endometritis of healthy animals.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0398-x) contains supplementary material, which is available to authorized users.

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Effect of ovarian hormones on the healthy equine uterus: a global gene expression analysis

Cited by 8 publications

References 73 publications

New insights in equine steroidogenesis: an in-depth look at steroid signaling in the placenta

New insights in equine steroidogenesis: an in-depth look at steroid signaling in the placenta

A comparison of the uterine proteome of mares in oestrus and dioestrus

Oestrous cycle-dependent equine uterine immune response to induced infectious endometritis

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