Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus-oocyte complex.

Wit, A.A.C. de; Wurth, Y.A.; Kruip, Th.A.M.

doi:10.2527/2000.7851277x

Cited by 97 publications

(72 citation statements)

References 28 publications

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“…These data indicate that the overgrowth of dominant follicles may have a negative impact on oocyte quality. The grade system for COCs to some extent can reflect the maturation state [12][13][14]. As the proportion of dominant follicles increased, the ratio of mature COCs (grade II) increased, and the ratio of aging COCs (grade III) also increased.…”

Section: Discussionmentioning

confidence: 99%

Association between the proportion of dominant follicles and oocyte developmental competence

et al. 2014

J Assist Reprod Genet

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Purpose To explore the optimal timing for hCG triggering by investigating the impact of different proportion of dominant follicles on the oocyte developmental competence. Methods One hundred ninety-eight infertile women were divided into three groups according to the proportion of dominant follicles on hCG day: (1) low: <15 % (n=66); (2) middle: 15-27 % (n=66); (3) high: >27 % (n=66). The grouping criteria were the bottom and top tertiles of the proportion of dominant follicles. Results The gonadotropin dosage, duration and maximum follicle diameter in the low proportion group were lower than those in the middle and high proportion groups. Oocyte maturation and the abnormal fertilization rate in the low proportion group were lower than those in the middle and high proportion groups. The normal fertilization rate did not differ among the three groups. The cleavage rate and number of transferable embryos in the low proportion group were significantly higher than those in the high proportion group. The high-quality embryo rate, implantation rate, and pregnancy rate in the low proportion group were significantly higher than those in the middle and high proportion groups.Conclusions A high proportion of dominant follicles are closely associated with impaired oocyte developmental competence and low pregnancy rate. These findings suggest that follicular overgrowth induced by delayed hCG triggering may undermine oocyte developmental competence and the proportion of dominant follicles may be a potential parameters for hCG triggering.

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Section: Discussionmentioning

confidence: 99%

Association between the proportion of dominant follicles and oocyte developmental competence

et al. 2014

J Assist Reprod Genet

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“…Luego se procedió a la colección de los complejos cúmulo-ovocitos (CCOs) fueron obtenidos mediante aspiración de folículos 7mm (De Wit et al, 2000). Con la ayuda de un estereomicroscopio (400x), se procedió a la búsqueda y selección de ovocitos con citoplasma homogéneo finamente granulado y uniforme, el cual debe llenar el espacio delimitado por la zona pelúcida (Bertoldo et al, 2010).…”

Section: Materiales Y Métodosunclassified

Efecto de la Suplementación del Medio de Maduración con Cisteamina y de Dos Medios de Cultivo (KSOMaa y SOF) en la Fecundación in vitro de Ovocitos Bovinos

Quintanilla

Huanca

Córdova

et al. 2015

Rev. investig. vet. Perú

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RESUMENSe evaluó el efecto de la cisteamina en el medio de maduración sobre la tasa de división posfecundación y el efecto de los medios de cultivo KSOMaa y SOF sobre la tasa de desarrollo embrionario in vitro hasta los 8 días posfecundación. Se utilizaron 680 ovocitos procedentes de ovarios de hembras bovinas distribuidos al azar en dos tratamientos: T 1 (n=321) medio de maduración TCM-199 y T 2 (n=359) medio de maduración TCM-199 adicionado con cisteamina 100 mM. Los ovocitos fueron fecundados a las 24 h con semen congelado de un solo toro y cultivados en medio KSOMaa por 18 h, evaluán-dose la tasa de división 72 h después. En la segunda fase, luego del primer cultivo, ambos grupos se subdividieron en dos grupos, donde el primero se cultivó con medio KSOMaa y el segundo con medio SOF hasta el día 8 posfecundación. Los resultados de la primera fase indican una tasa de división de 43.6% para el grupo control y 46.0% para el grupo suplementado con cisteamina, sin encontrar diferencia significativa. En la fase 2, se obtuvo una tasa de 18.0% de blastocistos para el tratamiento TCM-199 + KSOMaa y 20.9% para TCM-199 c/ cisteamina + KSOMaa (p<0.05), y 15.8% para TCM-199 + KSOMaa y SOF y 18.7% para TCM-199 c/ cisteamina + KSOMaa y SOF (p<0.05). Los resultados indican que la adición de cisteamina al medio de cultivo mejoró la tasa de desarrollo embrionario in vitro hasta el estadio de blastocisto en ovocitos madurados en medio TCM 199, no habiendo diferencias por el uso de los medios de cultivo.Palabras clave: fecundación in vitro, cisteamina, medio SOF, medio KSOM, bovino

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“…This was first demonstrated in the sheep, wherein oocytes from small atretric follicles retain the ability to yield blastocysts in vitro (Moor & Trounson, 1977). Conversely, there are detriments to the oocyte (as manifested by degenerative changes) in late stage atretic follicles with high levels of apoptotic granulosa cells (Blondin & Sirard, 1995;de Wit et al, 2000;Feng et al, 2007;Zeuner et al, 2003). Nevertheless, follicles with a high degree of atresia, as evidenced by apoptosis of the membrane granulosa and cumulusoocyte-complex (COC), still have the capacity to result in embryonic development (Hagemann et al, 1999).…”

Section: Relationship Between Follicular Atresia and The Oocytementioning

confidence: 99%

Ovarian Follicular Atresia

Townson¹,

Combelles²

2012

Basic Gynecology - Some Related Issues

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Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus-oocyte complex.

Cited by 97 publications

References 28 publications

Association between the proportion of dominant follicles and oocyte developmental competence

Association between the proportion of dominant follicles and oocyte developmental competence

Efecto de la Suplementación del Medio de Maduración con Cisteamina y de Dos Medios de Cultivo (KSOMaa y SOF) en la Fecundación in vitro de Ovocitos Bovinos

Ovarian Follicular Atresia

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