Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes

Love, L.B.; Choi, Young Ho; Love, Charles C.; Varner, D.D.; Hinrichs, Katrin

doi:10.1016/s0093-691x(02)01133-0

Cited by 48 publications

(33 citation statements)

References 18 publications

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“…The increased meiotic competence observed in Ex oocytes in this study is consistent with results previously obtained in our laboratory (Alm & Hinrichs 1996, Hinrichs & Williams 1997, Hinrichs & Schmidt 2000, Choi et al 2002b, Love et al 2003. Other laboratories have reported that Ex oocytes have the same maturation rate as do Cp oocytes (Del Campo et al 1995, Dell'Aquila et al 1997b, 2001, Choi et al 2000.…”

Section: Discussionsupporting

confidence: 92%

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

Choi

Love²,

Varner³

et al. 2004

Reproduction

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This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact) and duration of in vitro maturation (24, 30 or 42 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI). The effect of manipulation temperature (room temperature vs 37 8C) at the time of ICSI and concentration of glucose (0.55 vs 5.5 mM) during embryo culture was also investigated. The nuclear maturation rates of expanded (Ex) oocytes were significantly (P < 0.001) higher than those of compact (Cp) oocytes at all maturation times (61-72 vs 23 -25% respectively). Forty-eight hours after ICSI of mature Ex oocytes, the rate of cleavage with normal nuclei was significantly (P < 0.05) higher for oocytes matured for 24 h than for those matured for 30 or 42 h (73 vs 57-59% respectively). For Cp oocytes, the morphologic cleavage rates for oocytes matured for 30 h were significantly higher (P < 0.05) than for those matured for 24 or 42 h (86 vs 55-61% respectively). The overall proportion of embryos having more than four normal nuclei at 48 h culture was significantly higher (P < 0.05) for Cp than for Ex oocytes. Manipulation temperature did not affect development of embryos from Ex or Cp oocytes at 96 h after ICSI. Culture in high-glucose medium significantly increased morphologic cleavage of Cp, but not Ex, oocytes (P < 0.05). Embryos from Cp oocytes had a significantly higher average nucleus number after 96-h culture than did embryos from Ex oocytes. These data indicate that developmental competence differs between Ex and Cp equine oocytes, and is differentially affected by the duration of maturation and by composition of embryo culture media.

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Section: Discussionsupporting

confidence: 92%

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

Choi

Love²,

Varner³

et al. 2004

Reproduction

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“…These findings are in agreement with those reported by Love et al (2003) and Yuge et al (2003), but contrast those of Wongsrikeao et al (2005) and Evecen et al (2010). Some disparities might be caused by long storage time, which was studied by these investigators.…”

Section: Resultssupporting

confidence: 92%

HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

Bohlooli¹,

Bozoglu²,

Cedden³

2016

SA J. An. Sci.

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This study was conducted to investigate the effects of ovary transportation in a semi-complex medium containing HEPES at different temperatures on the developmental competence and the quality of in vitroproduced embryos. The cattle ovaries were transported in normal saline (NS), phosphate buffer saline (PBS), K simplex optimization medium (KSOM), Chatot-Ziomek-Bavister medium (CZB) and Charles Rosenkrans medium (CR1) at various temperatures (38 ºC, 25 ºC and 4 ºC). The developmental competence of retrieved cumulus oocyte complexes (COCs) was evaluated by maturation, fertilization, morula and blastocyst formation and numbers of inner cell mass (ICM) and trophectoderm (TE) cells. The COC maturation rate was affected by medium and temperature. It was found that 4 ºC resulted in a higher maturation (81.0 ± 4.75) rate than other transportation temperatures. The CR1 (80.5 ± 6.66) and KSOM (80.2 ± 6.15) gave a better maturation rate than the others. Fertilization rate, which was evaluated by cleavage rate, was not affected by transportation temperature. However, the transporting medium had a significant effect on the fertilization rate. Moreover, CR1 (43.6 ± 4.60), KSOM (43.2 ± 4.86) and CZB (41.1 ± 4.86) media gave higher percentages of cleaved embryos. There was no significant difference in morula and blastocyst formation rate or in ICM and TE cell counts regarding transportation factors. In conclusion, the transport of ovaries in CR1 at 4 ºC is effective for maintaining early developmental competence of cattle oocytes.

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“…Cat oocytes recovered from ovaries stored at 4 C for 24 h are capable of m a i n t a i n i n g t h e c o m p e t e n c e o f I V M a n d development after IVF [20,23]. The storage of horse ovaries at 25 C and 35 C for 5-8 h and 3-15 h, respectively, had no effects on the meiotic competence of oocytes [6,24,25], but a lower temperature (4 C) was detrimental as compared with room temperature [26]. Also, sheep oocytes were sensitive to cooling to 20 C at various stages of meiosis of oocytes, resulting in the induction of chromosomal abnormalities [27].…”

Section: Discussionmentioning

confidence: 99%

Effects of Ovary Storage Time and Temperature on DNA Fragmentation and Development of Porcine Oocytes

Wongsrikeao

Otoi

Karja

et al. 2005

Journal of Reproduction and Development

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Abstract. The present study was conducted to investigate the effects of storage time and temperature of porcine ovaries on the quality and nuclear maturation in vitro of oocytes obtained from stored ovaries and their subsequent development after in vitro fertilization. The ovaries were stored in physiological saline for 0, 3, 6, 9 and 12 h at various temperatures (4, 15, 25 and 35 C). The pH of follicular fluid obtained from the ovaries, DNA fragmentation of the oocyte nucleus and meiotic competence of oocytes were examined. Some oocytes from ovaries stored at 15, 25 and 35 C for 6 h were fertilized in vitro, and then cultured for 7 days to examine the ability of embryos to develop to the blastocyst stage. When the ovaries were stored at 35 C, the pH of follicular fluid decreased and the proportions of oocytes with DNA fragmented nuclei increased as the storage time was prolonged, and the storage of ovaries for 6, 9 and 12 h resulted in lower maturation rates of oocytes. When the ovaries were stored at 4, 15, 25 and 35 C for 6 h, the storage at higher temperatures (≥15 C) decreased the pH of follicular fluid and induced nucleic DNA fragmentation in higher proportions of oocytes. None of the oocytes from ovaries stored at 4 C reached metaphase II. The storage of ovaries at 15 C reduced the rates of in vitro fertilized oocytes and subsequent embryo development, but there were no significant differences in the rates of fertilization and blastocyst formation between oocytes from ovaries stored at 25 C and 35 C. Our findings indicate that the storage of ovaries at 25-35 C for 6 h is effective for maintaining the developmental competence of porcine oocytes even though the development rates were lower than those of ovaries stored at 35 C for 3 h.

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Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes

Cited by 48 publications

References 18 publications

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

HEPES buffer in ovary-transportation medium influences developmental competence of cattle oocytes

Effects of Ovary Storage Time and Temperature on DNA Fragmentation and Development of Porcine Oocytes

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