Effect of overexpression of citrus 9-cis-epoxycarotenoid dioxygenase 3 (CsNCED3) on the physiological response to drought stress in transgenic tobacco

Pedrosa, Andresa Muniz; Cidade, Luciana C.; Martins, Cristina P. S.; Macedo, Amanda Ferreira; Neves, Diana Matos; Gomes, Fábio Pinto; Floh, Eny Iochevet Segal; Costa, Márcio Gilberto Cardoso

doi:10.4238/gmr16019292

Cited by 23 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sucrose transporters are responsible for phloem loading, transport and unloading of sucrose from source to sink 36 . NCED genes encode key enzymes for the synthesis of ABA, and are induced under drought stress 37 , 38 . The transcript levels of NcSUT4 were quantified in the samples of different developmental stages and different tissues, using two stable reference genes and two unstable reference genes as endogenous controls, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba

Huang

Jiang

Siwen

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Neolamarckia cadamba is an economically-important fast-growing tree species in South China and Southeast Asia. As a prerequisite first step for future gene expression studies, we have identified and characterized a series of stable reference genes that can be used as controls for quantitative real time PCR (qRT-PCR) expression analysis in this study. The expression stability of 15 candidate reference genes in various tissues and mature leaves under different conditions was evaluated using four different algorithms, i.e., geNorm, NormFinder, BestKeeper and RefFinder. Our results showed that SAMDC was the most stable of the selected reference genes across the set of all samples, mature leaves at different photosynthetic cycles and under drought stress, whereas RPL10A had the most stable expression in various tissues. PGK and RPS25 were considered the most suitable reference for mature leaves at different developmental stages and under cold treatment, respectively. Additionally, the gene expression profiles of sucrose transporter 4 (NcSUT4), and 9‐cis‐epoxycarotenoid dioxygenase 3 (NcNCED3) were used to confirm the validity of candidate reference genes. Collectively, our study is the first report to validate the optimal reference genes for normalization under various conditions in N. cadamba and will benefit the future discovery of gene function in this species.

show abstract

Section: Resultsmentioning

confidence: 99%

Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba

Huang

Jiang

Siwen

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Moreover, OsNCED3 -overexpressing plants had enhanced NaCl and water stress tolerance (Figures 9A–D ). Several other NCED genes, including VP14 (Tan et al, 1997 ), AtNCED3 (Frey et al, 2012 ), CsNCED3 (Pedrosa et al, 2017 ), SgNCED (Yang and Guo, 2007 ), and LeNCED1 (Tung et al, 2008 ) were previously reported to alter water stress sensitivity if expression was inhibited or overexpressed in plants. Under water stress conditions, the plants underwent a physiological transformation to adapt to the severe environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

et al. 2018

View full text Add to dashboard Cite

Although abscisic acid (ABA) is an important hormone that regulates seed dormancy, stomatal closure, plant development, as well as responses to environmental stimuli, the physiological mechanisms of ABA response to multiple stress in rice remain poorly understood. In the ABA biosynthetic pathway, 9-cis-epoxycarotenoid dioxygenase (NCED) is the key rate-limiting enzyme. Here, we report important functions of OsNCED3 in multi-abiotic stress tolerance in rice. The OsNCED3 is constitutively expressed in various tissues under normal condition, Its expression is highly induced by NaCl, PEG, and H2O2 stress, suggesting the roles for OsNCED3 in response to the multi-abiotic stress tolerance in rice. Compared with wild-type plants, nced3 mutants had earlier seed germination, longer post-germination seedling growth, increased sensitivity to water stress and H2O2 stress and increased stomata aperture under water stress and delayed leaf senescence. Further analysis found that nced3 mutants contained lower ABA content compared with wild-type plants, overexpression of OsNCED3 in transgenic plants could enhance water stress tolerance, promote leaf senescence and increase ABA content. We conclude that OsNCED3 mediates seed dormancy, plant growth, abiotic stress tolerance, and leaf senescence by regulating ABA biosynthesis in rice; and may provide a new strategy for improving the quality of crop.

show abstract

“…NCED has been shown to contribute to increased ABA levels and abiotic stress tolerance in plants [20]. NCED genes such as VP14 [21], SgNCED [57], AtNCED3 [27] and CsNCED3 [58] were previously reported to alter water stress sensitivity if expression was inhibited or promoted in plants. In rice, five members of the NCED family (OsNCED1-5) have been characterized [59,60].…”

Section: Osmads23 Promotes Aba Biosynthesis By Activating Osnceds Directlymentioning

confidence: 99%

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

et al. 2021

PLoS Genet

View full text Add to dashboard Cite

Some of MADS-box transcription factors (TFs) have been shown to play essential roles in the adaptation of plant to abiotic stress. Still, the mechanisms that MADS-box proteins regulate plant stress response are not fully understood. Here, a stress-responsive MADS-box TF OsMADS23 from rice conferring the osmotic stress tolerance in plants is reported. Overexpression of OsMADS23 remarkably enhanced, but knockout of the gene greatly reduced the drought and salt tolerance in rice plants. Further, OsMADS23 was shown to promote the biosynthesis of endogenous ABA and proline by activating the transcription of target genes OsNCED2, OsNCED3, OsNCED4 and OsP5CR that are key components for ABA and proline biosynthesis, respectively. Then, the convincing evidence showed that the OsNCED2-knockout mutants had lower ABA levels and exhibited higher sensitivity to drought and oxidative stress than wild type, which is similar to osmads23 mutant. Interestingly, the SnRK2-type protein kinase SAPK9 was found to physically interact with and phosphorylate OsMADS23, and thus increase its stability and transcriptional activity. Furthermore, the activation of OsMADS23 by SAPK9-mediated phosphorylation is dependent on ABA in plants. Collectively, these findings establish a mechanism that OsMADS23 functions as a positive regulator in response to osmotic stress by regulating ABA biosynthesis, and provide a new strategy for improving drought and salt tolerance in rice.

show abstract

Effect of overexpression of citrus 9-cis-epoxycarotenoid dioxygenase 3 (CsNCED3) on the physiological response to drought stress in transgenic tobacco

Cited by 23 publications

References 18 publications

Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba

Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

OsMADS23 phosphorylated by SAPK9 confers drought and salt tolerance by regulating ABA biosynthesis in rice

Contact Info

Product

Resources

About