Effect of Oxidative Stress on Reproduction and Development

Guerriero, Giulia; D’Errico, Gerardino

doi:10.3390/antiox11020312

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…Impaired synaptic vesicle transport and exocytosis processes can further disrupt neurotransmitter release ( Guo et al, 2017 ; Rangaraju et al, 2019 ) ( Figure 2G ). Moreover, the development can also be affected by autophagy ( Aburto et al, 2012 ) ( Figure 2H ) and oxidative stress ( Guerriero and D'Errico, 2022 ) ( Figure 2I ).…”

Section: Discussionmentioning

confidence: 99%

Temperature- and chemical-induced neurotoxicity in zebrafish

Toni,

Arena,

Cioni

et al. 2023

Front. Physiol.

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Throughout their lives, humans encounter a plethora of substances capable of inducing neurotoxic effects, including drugs, heavy metals and pesticides. Neurotoxicity manifests when exposure to these chemicals disrupts the normal functioning of the nervous system, and some neurotoxic agents have been linked to neurodegenerative pathologies such as Parkinson’s and Alzheimer’s disease. The growing concern surrounding the neurotoxic impacts of both naturally occurring and man-made toxic substances necessitates the identification of animal models for rapid testing across a wide spectrum of substances and concentrations, and the utilization of tools capable of detecting nervous system alterations spanning from the molecular level up to the behavioural one. Zebrafish (Danio rerio) is gaining prominence in the field of neuroscience due to its versatility. The possibility of analysing all developmental stages (embryo, larva and adult), applying the most common “omics” approaches (transcriptomics, proteomics, lipidomics, etc.) and conducting a wide range of behavioural tests makes zebrafish an excellent model for neurotoxicity studies. This review delves into the main experimental approaches adopted and the main markers analysed in neurotoxicity studies in zebrafish, showing that neurotoxic phenomena can be triggered not only by exposure to chemical substances but also by fluctuations in temperature. The findings presented here serve as a valuable resource for the study of neurotoxicity in zebrafish and define new scenarios in ecotoxicology suggesting that alterations in temperature can synergistically compound the neurotoxic effects of chemical substances, intensifying their detrimental impact on fish populations.

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Section: Discussionmentioning

confidence: 99%

Temperature- and chemical-induced neurotoxicity in zebrafish

Toni,

Arena,

Cioni

et al. 2023

Front. Physiol.

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Efficient Production of Epoxy‐Norbornane from Norbornene by an Engineered P450 Peroxygenase

et al. 2022

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Epoxy-norbornane (EPO-NBE) is a crucial building block for the synthesis of various biologically active heterocyclic systems. To develop an efficient protocol for producing EPO-NBE using norbornene (NBE) as a substrate, cytochrome P450 enzyme from Pseudomonas putida (CYP238A1) was examined and its crystal structure (PDB code: 7X53) was resolved. Molecular mechanism analysis showed a high energy barrier related to iron-alkoxy radical complex formation. Therefore, a protein engineering strategy was developed and an optimal CY-P238A1 NPV variant containing a local hydrophobic "fence" at the active site was obtained, which increased the H 2 O 2 -dependent epoxidation activity by 7.5-fold compared with that of CYP238A1 WT . Among the "fence", Glu255 participates in an efficient proton transfer system. Whole-cell transformation using CYP238A1 NPV achieved an EPO-NBE yield of 77.6 g • L À 1 in a 30-L reactor with 66.3 % conversion. These results demonstrate the potential of this system for industrial production of EPO-NBE and provides a new biocatalytic platform for epoxidation chemistry.

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