Effect of Oxidized Frying Oil and Vitamin C Levels on the Hepatic Xenobiotic-Metabolizing Enzyme System of Guinea Pigs.

Liu, Jen‐Fang; Lee, Ya-Wen; Chang, Feng-Chen

doi:10.3177/jnsv.46.137

Cited by 12 publications

(7 citation statements)

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“…Previous studies have shown that the glu tathione level typically decreases in OFO-fed guinea pigs (10). Hepatic microsomal-CYP450 enzymes in OFO-fed guinea pigs OFO feeding stimulated an increase in hepatic micro somal protein, total CYP450 content, and NADPH-cy tochrome c reductase activity in the guinea pigs ( Table 5); results that are similar to those of a previous study (11). For animals, OFO is classified as belonging to the fat-soluble xenobiotic category, and it can induce en zyme activity in the detoxification system.…”

Section: Resultssupporting

confidence: 84%

“…In this experi mental model, guinea pigs were used, while the major ity of researchers used rats (10,11,(26)(27)(28). Sies et al suggested that oxidized oil contains a cyclic monomer that induces CYP2B activity for the Wistar rat (26).…”

Section: Resultsmentioning

confidence: 99%

“…The effect of an OFO-feeding regime upon the induc tion of CYP450 enzymes in rats and guinea pigs has been studied by various workers (11)(12)(13). The changes in activity of CYP450 enzymes may lead to changes in drug pharmacokinetics and chemical carcinogenesis, However, the form of the CYP450 isozyme induced by an OFO diet is still unknown.…”

mentioning

confidence: 99%

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Forms of Cytochrome P450 in the Liver Microsome of Oxidized Frying Oil-fed Guinea Pigs.

Liu

Chang

2000

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

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Forms of Cytochrome P450 in the Liver Microsome of Oxidized Frying Oil-fed Guinea Pigs.

Liu

Chang

2000

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Previous studies have shown that administration of oxidized oils increases gene expression and activities of various enzymes involved in xenobiotic metabolism and stress response in the liver of rats and guinea pigs [14-18]. Hepatic xenobiotic metabolism and stress response is mainly controlled by nuclear factor erythroid-derived 2-like 2 (Nrf2), a master transcription factor shown to regulate more than 200 genes, including those involved in phase II detoxification and antioxidant defense [19].…”

Section: Introductionmentioning

confidence: 99%

Dietary moderately oxidized oil activates the Nrf2 signaling pathway in the liver of pigs

et al. 2012

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BackgroundPrevious studies have shown that administration of oxidized oils increases gene expression and activities of various enzymes involved in xenobiotic metabolism and stress response in the liver of rats and guinea pigs. As these genes are controlled by nuclear factor erythroid-derived 2-like 2 (Nrf2), we investigated the hypothesis that feeding of oxidized fats causes an activation of that transcription factor in the liver which in turn activates the expression of antioxidant, cytoprotective and detoxifying genes.MethodsTwenty four crossbred pigs were allocated to two groups of 12 pigs each and fed nutritionally adequate diets with either fresh rapeseed oil (fresh fat group) or oxidized rapeseed oil prepared by heating at a temperature of 175°C for 72 h (oxidized fat group).ResultsAfter 29 days of feeding, pigs of the oxidized fat group had a markedly increased nuclear concentration of the transcription factor Nrf2 and a higher activity of cellular superoxide dismutase and T4-UDP glucuronosyltransferase in liver than the fresh fat group (P < 0.05). In addition, transcript levels of antioxidant and phase II genes in liver, like superoxide dismutase 1, heme oxygenase 1, glutathione peroxidase 1, thioredoxin reductase 1, microsomal glutathione-S-transferase 1, UDP glucuronosyltransferase 1A1 and NAD(P)H:quinone oxidoreductase 1 in the liver were higher in the oxidized fat group than in the fresh fat group (P < 0.05). Moreover, pigs of the oxidized fat group had an increased hepatic nuclear concentration of the transcription factor NF-κB which is also an important transcription factor mediating cellular stress response.ConclusionThe present study shows for the first time that administration of an oxidized fat activates the Nrf2 in the liver of pigs which likely reflects an adaptive mechanism to prevent cellular oxidative damage. Activation of the NF-κB pathway might also contribute to this effect of oxidized fat.

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“…According to Wang et al (26), indirect mutagens, such as B(a)P or AFB1, must be activated by CYP450 before they exhibit mutagenic abilities. OFO feeding produced marked xenobiotic-metabolizing enzyme induction, including CYP450, in previous studies (27,28), and polyphenol was found to inhibit the activity of CYP450 (data not shown), and might therefore have the poten tial to decrease many CYP450-mediated cancers or chronic disease. The antimutagenic ability of H. cordata may also reflect the inhibitory effect of polyphenols on CYP450 activity.…”

Section: Methodsmentioning

confidence: 72%

A Study of the Antioxidative and Antimutagenic Effects of Houttuynia cordata Thunb. Using an Oxidized Frying Oil-Fed Model

Chen

Liu

Chen

et al. 2003

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Effect of Oxidized Frying Oil and Vitamin C Levels on the Hepatic Xenobiotic-Metabolizing Enzyme System of Guinea Pigs.

Cited by 12 publications

References 12 publications

Forms of Cytochrome P450 in the Liver Microsome of Oxidized Frying Oil-fed Guinea Pigs.

Forms of Cytochrome P450 in the Liver Microsome of Oxidized Frying Oil-fed Guinea Pigs.

Dietary moderately oxidized oil activates the Nrf2 signaling pathway in the liver of pigs

A Study of the Antioxidative and Antimutagenic Effects of Houttuynia cordata Thunb. Using an Oxidized Frying Oil-Fed Model

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