Effect of Oxygen Concentration on Free Radical-Induced Cytotoxicity

Chen, Zhi Hua; Saito, Yoshiro; Yoshida, Yasukazu; Niki, Etsuo

doi:10.1271/bbb.80002

Cited by 25 publications

(30 citation statements)

References 20 publications

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“…However, we did not detect any DL increase in our experiments with varying levels of superoxide generators. Moreover, H 2 O 2 appears to exert its cytotoxic effects on Jurkat cells without producing significant levels of lipid peroxides [45], and in our work the QC10*-pretreatment, which offered substantial protection against H 2 O 2 and hence would prevent the lipoperoxidation effects of H 2 O 2 (if any), did not ameliorate the DL reduction observed after the H 2 O 2 treatment. Taken together, these findings suggest that DL in Jurkat cells is not related appreciably to lipid peroxidation or production of singlet oxygen.…”

Section: Discussioncontrasting

confidence: 47%

Effects of Menadione, Hydrogen Peroxide, and Quercetin on Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T-Cells

Băran

Ganea

Scordino

et al. 2010

Cell Biochem Biophys

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Menadione (MD) is an effective cytotoxic drug able to produce intracellularly large amounts of superoxide anion. Quercetin (QC), a widely distributed bioflavonoid, can exert both antioxidant and pro-oxidant effects and is known to specifically inhibit cell proliferation and induce apoptosis in different cancer cell types. We have investigated the relation between delayed luminescence (DL) induced by UV-laser excitation and the effects of MD, hydrogen peroxide, and QC on apoptosis and cell cycle in human leukemia Jurkat T-cells. Treatments with 500 μM H₂O₂ and 250 μM MD for 20 min produced 66.0 ± 4.9 and 46.4 ± 8.6% apoptotic cell fractions, respectively. Long-term (24 h) pre-exposure to 5 μM, but not 0.5 μM QC enhanced apoptosis induced by MD, whereas short-term (1 h) pre-incubation with 10 μM QC offered 50% protection against H₂O₂-induced apoptosis, but potentiated apoptosis induced by MD. Since physiological levels of QC in the blood are normally less than 10 μM, these data can provide relevant information regarding the benefits of flavonoid-combined treatments of leukemia. All the three drugs exerted significant effects on DL. Our data are consistent with (1) the involvement of Complex I of the mitochondrial respiratory chain as an important source of delayed light emission on the 10 μs-10 ms scale, (2) the ability of superoxide anions to quench DL on the 100 μs-10 ms scale, probably via inhibition of reverse electron transfer at the Fe/S centers in Complex I, and (3) the relative insensitivity of DL to intracellular OH• and H₂O₂ levels.

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Section: Discussioncontrasting

confidence: 47%

Effects of Menadione, Hydrogen Peroxide, and Quercetin on Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T-Cells

Băran

Ganea

Scordino

et al. 2010

Cell Biochem Biophys

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“…The lipid peroxidation products were significantly higher in the group B compared with both the control group A and group C. This means that intra-abdominal hypertension caused significantly higher oxidative damage to the fatty acids of the muscle fibers, confirming their increased sensitivity to the oxidative stress caused by hypoxia [37,38]. Similarly, protein carbonyl content, as a biomarker of protein oxidative modification, was also higher in group B compared with both groups A and C. No difference was found between groups A and C. Previous studies have shown that aging, alcohol abuse, and congestive heart failure are associated with increased protein carbonyl content [39][40][41][42].…”

Section: Discussionmentioning

confidence: 66%

Can Chronic Intra-Abdominal Hypertension Cause Oxidative Stress to the Abdominal Wall Muscles? An Experimental Study

Kotidis

Papavramidis

Ioannidis

et al. 2012

Journal of Surgical Research

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“…Here, we show that the lower proliferative index at low O 2 is in fact a result of a delay of the initial response together with an increased apoptotic rate. This latter can result from the increased cytotoxicity of activation-induced free radicals in PhysO 2 within certain T cell subsets: Here, we identified these as naïve and TEMRA CD8ϩ T cells and naïve and CM CD4ϩ T cells [22]. This suggests that T cell death at low oxygen is competing with proliferation until selection of the subsets most fitted for survival at low O 2 .…”

Section: Discussionmentioning

confidence: 73%

Induction of HIF-1α and the glycolytic pathway alters apoptotic and differentiation profiles of activated human T cells

Larbi¹,

Zelba²,

Goldeck³

et al. 2009

Journal of Leukocyte Biology

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The majority of in vitro studies involving lymphocytes is performed in AtmO(2), and the PhysO(2) that T cells encounter are variable but commonly much lower. Previous studies showed changed kinetics and delayed proliferation of human T cells at PhysO(2). Here, we show that CD3/CD28-dependent T cell activation induces faster cell cycling at AtmO(2) than at PhysO(2) (here taken to be 2%). Concomitantly with HIF-1alpha expression, we observed a switch in the T cell respiratory pathway toward glycolysis at PhysO(2). Thus, modulating available glucose levels showed that at PhysO(2), T cells rely more on glycolysis, associated with a higher phosphorylation of Akt(ser473). Although no difference in spontaneous apoptosis of resting cells was detected, it was increased significantly at PhysO(2) after T cell activation and was different within the different T cell subsets. This may explain at least partly the differently altered proliferation and subset distribution observed in CD4+ and CD8+ T cells as a result of differences in naïve and memory subset distribution. Together, these findings suggest that T cell activation thresholds, subsequent proliferative capacity, and susceptibility to apoptosis, hitherto studied in air and thought to be crucial for monitoring immune responsiveness, may require re-assessment.

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Effect of Oxygen Concentration on Free Radical-Induced Cytotoxicity

Cited by 25 publications

References 20 publications

Effects of Menadione, Hydrogen Peroxide, and Quercetin on Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T-Cells

Effects of Menadione, Hydrogen Peroxide, and Quercetin on Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T-Cells

Can Chronic Intra-Abdominal Hypertension Cause Oxidative Stress to the Abdominal Wall Muscles? An Experimental Study

Induction of HIF-1α and the glycolytic pathway alters apoptotic and differentiation profiles of activated human T cells

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